EDC3 (NM_025083) Human Untagged Clone

CAT#: SC110646

EDC3 (untagged)-Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDC3
• Synonyms: hYjeF_N2-15q23; LSM16; MRT50; YJDC; YJEFN2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110646 sequence for NM_025083 edited (data generated by NextGen Sequencing)
  ATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTC
  TATCAGGGAAGAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCT
  TTCCATAATGGAGTGAAGTGTCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACG
  GAGTTAAAAATTCTGGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCAT
  CAAACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAATGGCACA
  GGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAGAATATCCCTAAGAGG
  ACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGCAGCAACAGTGCTCAAAGAGC
  TATGTCGACAGGCACATGGAATCCTTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAAC
  TCCTGGTCATCTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAG
  AATGGCCAGATGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATTGAGGAGATCCCA
  GACACAGATTTTGATTTTGAAGGGAACCTGGCTCTTTTTGACAAGGCAGCTGTGTTTGAG
  GAGATTGATACCTATGAAAGGAGAAGTGGTACCCGTTCCCGGGGCATCCCAAATGAAAGG
  CCCACTCGGTACCGCCATGATGAGAACATCTTGGAGTCCGAGCCCATTGTCTATCGACGG
  ATCATAGTGCCCCACAACGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCA
  AGTATTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGGCTGACC
  CTTGAGCGGAGACTGGAGATGACAGGTGTGTGTGCCAGTCAGATGGCACTGACCCTCCTC
  GGAGGACCTAACAGGTTGAATCCCAAAAATGTTCACCAGAGGCCTACAGTGGCTCTACTG
  TGTGGACCTCATGTGAAGGGGGCTCAGGGTATCAGCTGTGGAAGGCACCTAGCCAACCAT
  GATGTCCAGGTCATCCTTTTCCTGCCCAATTTTGTCAAGATGTTGGAATCTATCACCAAT
  GAGCTGTCGCTCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAAAGATCTGCCC
  ACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGAGAACGTCTTCCTGCGC
  GATCAACCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACCAGAACCGGGCACCAGTACTC
  AGCATAGACCCTCCTGTGCATGAAGTCGAACAGGGCATTGATGCCAAATGGTCACTGGCA
  CTGGGCCTGCCTCTGCCACTGGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGC
  ATTCCCCAGCAGGTCTTCCAGGAGGTGGGCATCAACTACCACTCGCCCTTTGGCTGCAAG
  TTTGTTATCCCACTGCACTCTGCTTAG
  
  Clone variation with respect to NM_025083.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025083 unedited
  NGTTCNCATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCCGATGCT
  GTGGGGGTGGGCGTGGAGAGAATTCTTCTGTGGGTCCTCTGGTGTTGAGTGGTCGGCTTG
  GTGTGGTGTGCGGAGGAGCTCCAGGCCCGTCGGCGCGGAGTGGTCTCACGTGTGAAACAT
  GGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTCTA
  TCAGGGAAGAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCTTT
  CCATAATGGAGTGAAGTGTCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGA
  GTTAAAAATTCTGGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCATCA
  AACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAATGGCACAGG
  CAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAGAATATCCCTAAGAGGAC
  AGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGCAGCAACAGTGCTCAAAGAGCTA
  TGTCGACAGGCACATGGAATCCTTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTC
  CTGGTCATCTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAGTGGTTTANAGAAT
  GGCCAGATGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATGGAGGAGATCCCAGAC
  CCAGATTTTGATTTTGAAGGGAACCTGGCCTCTTTTTGACAGGCAGCTGTGTTTTGAGGA
  GATTGATCCCTATGAAAGGAGAAGTGGGTACCCGTTCCCGGGGCATTCCCAATGAAAGGC
  CCACTCTGTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025083 unedited
  GGCTTTCAGCTTTCTTTNCAGTGGCTGGTTGTTTGGTTGCCCTAAGAGCTGCATGAGTGC
  TAGGCTTCACCACGTGACTGGGGGCCCCTTGGGAACTGGGTACTATGGGCAGGATGCCCC
  TGAAAAGAACTGAAGACAGAGGAATCATACTTCTCTTTAATACCTCTGGGGAAGGCCCAG
  GCTAAGGATGAGGGCAGGGACCAGTCCCAGTGCCCCCTGGGGAGAGAAGAGGGAGAAGCT
  TGGGCACAAACTCCCAGTGGCCCTGCAAGGCTATCATCCCTGGATCTTGCTGGAGTGGAC
  AGTCTTCTGGGCCAGGGTTGTTCTAGCTTGTTTCTCACTGTACCTAATCAGGGGGCCTAA
  CTGCATTAACAGGCAGCCAGAACCTACCTACCAAAAAGCCTGTAACCTAGACTGCTCCTC
  AGATTTTGGCCAAGAGAGGGCCCCGGCTTGGGAGCTGCTTGGCCCTGAGAGTGAGCAGAG
  GCTCAGAGAATACACAGCACAAGGGTTACAGTCCCTGGCCTCTTCCCATCGCTGGAGACA
  ATTTAAGACTGAAGGGAAGATGAGAGGAGGGGCCAGCCCTCTACCCTGGCTCATCTGCTT
  CTGGCTATGTTCCTACAAGGGAAGTGATAACAGCCTGAAACCCGTGGTACCAGGGCCTAC
  CCTATGTCCACAATCCTTGGTGAGCCCTTCTGTCCACCCATTCACCCGCTCAGCCAGCCT
  GGGCAGACACAGAGCAGAAGCAGCCTGAAGCCCTGAAGCAGGCTTAAACACAAGTCCTTG
  ACAGGATCCCTGCCAGGATTCAGGGTAGATCCTGTTCTCCAGCCTCCAGGCCTANAGCTG
  GCACCTTAGATTGAGCTCANTGGGTCCTATGGTGGACCCCACAGCTACTCTCCAGACTAG
  CCTATTTGCCTCTTGAACAGGGGTGGCCCAGCTCCATTAACTGGCAATGGAAGTTCAACC
  ACAGAGCANACTGGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025083
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025083.2, NP_079359.2
• RefSeq Size: 3755 bp
• RefSeq ORF: 1527 bp
• Locus ID: 80153
• UniProt ID: Q96F86
• Cytogenetics: 15q24.1
• Domains: YjeF_N
• Protein Pathways: RNA degradation
• Gene Summary: This gene encodes a protein that is important in mRNA degradation. The encoded protein is a component of a decapping complex that promotes efficient removal of the monomethylguanosine (m7G) cap from mRNAs, as part of the 5' to 3' mRNA decay pathway. Mutations in this gene have been identified in human patients with an autosomal recessive form of intellectual disability. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same isoform (1).