SCDGFB (PDGFD) (NM_033135) Human Untagged Clone

CAT#: SC110642

PDGFD (untagged)-Human platelet derived growth factor D (PDGFD), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCDGFB
• Synonyms: IEGF; MSTP036; SCDGF-B; SCDGFB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033135, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCACCGGCTCATCTTTGTCTACACTCTAATCTGCGCAAACTTTTGCAGCTGTCGGGACACTTCTGCAA
  CCCCGCAGAGCGCATCCATCAAAGCTTTGCGCAACGCCAACCTCAGGCGAGATGACTTGTACCGAAGAGA
  TGAGACCATCCAGGTGAAAGGAAACGGCTACGTGCAGAGTCCTAGATTCCCGAACAGCTACCCCAGGAAC
  CTGCTCCTGACATGGCGGCTTCACTCTCAGGAGAATACACGGATACAGCTAGTGTTTGACAATCAGTTTG
  GATTAGAGGAAGCAGAAAATGATATCTGTAGGTATGATTTTGTGGAAGTTGAAGATATATCCGAAACCAG
  TACCATTATTAGAGGACGATGGTGTGGACACAAGGAAGTTCCTCCAAGGATAAAATCAAGAACGAACCAA
  ATTAAAATCACATTCAAGTCCGATGACTACTTTGTGGCTAAACCTGGATTCAAGATTTATTATTCTTTGC
  TGGAAGATTTCCAACCCGCAGCAGCTTCAGAGACCAACTGGGAATCTGTCACAAGCTCTATTTCAGGGGT
  ATCCTATAACTCTCCATCAGTAACGGATCCCACTCTGATTGCGGATGCTCTGGACAAAAAAATTGCAGAA
  TTTGATACAGTGGAAGATCTGCTCAAGTACTTCAATCCAGAGTCATGGCAAGAAGATCTTGAGAATATGT
  ATCTGGACACCCCTCGGTATCGAGGCAGGTCATACCATGACCGGAAGTCAAAAGTTGACCTGGATAGGCT
  CAATGATGATGCCAAGCGTTACAGTTGCACTCCCAGGAATTACTCGGTCAATATAAGAGAAGAGCTGAAG
  TTGGCCAATGTGGTCTTCTTTCCACGTTGCCTCCTCGTGCAGCGCTGTGGAGGAAATTGTGGCTGTGGAA
  CTGTCAACTGGAGGTCCTGCACATGCAATTCAGGGAAAACCGTGAAAAAGTATCATGAGGTATTACAGTT
  TGAGCCTGGCCACATCAAGAGGAGGGGTAGAGCTAAGACCATGGCTCTAGTTGACATCCAGTTGGATCAC
  CATGAACGATGTGATTGTATCTGCAGCTCAAGACCACCTCGATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033135 unedited
  GGTTCAAATATTTGTATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCAAGG
  AAGTGCGGCACCCACACGCGCTCGNGAAGTTCAGCATGCAGGAAGTTTGGGGAGAGCTCG
  GCGATTAGCACAGCGACCCGGGCCAGCGCAGGGCGAGCGCAGGCGGCGAGAGCGCAGGGC
  GGCGCGGCGTCGGTCCCGGGAGCAGAACCCGGCTTTTTCTTGGAGCGACGCTGTCTCTAG
  TCGCTGATCCCAAATGCACCGGCTCATCTTTGTCTACACTCTAATCTGCGCAAACTTTTG
  CAGCTGTCGGGACACTTCTGCAACCCCGCAGAGCGCATCCATCAAAGCTTTGCGCAACGC
  CAACCTCAGGCGAGATGACTTGTACCGAAGAGATGAGACCATCCAGGTGAAAGGAAACGG
  CTACGTGCAGAGTCCTAGATTCCCGAACAGCTACCCCAGGAACCTGCTCCTGACATGGCG
  GCTTCACTCTCAGGAGAATACACGGATACAGCTAGTGTTTGACAATCAGTTTGGATTAGA
  GGAAGCAGAAAATGATATCTGTAGGTATGATTTTGTGGAAGTTGAAGATATATCCGAAAC
  CAGTACCATTATTAGAGGACGATGGTGTGGACACAAGGAAGTTCCTCCAAGGATAAAATC
  AAGAACGAACCAAATTAAAATCACATTCAAGTCCGATGACTACTTTGTGGCTAAACCTGG
  ATTCAAGATTTATTATTCTTTGCTGGAAGATTTCCACCCGCAGCAGCTTCAGAGACCAAC
  TGGGAATCTGTNCACAGCTCTATTTCAGGNGTATNNCTATACTCTNCATCAGTNACGGGA
  TCCACTCTGATTGCGGATGCTCTGGACAAAAAAATGCAGAATTGATACAGTGGAGATCTG
  CTCAGTACTTCATCCAGAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033135 unedited
  TTTGGACCGGCGGCCGCAATCTTTGATCGAGTTTTTTTTTTTTTTTTTTGTTCAAAAGCA
  TATTTATTTTTCATGAAGATAAGAGGCATATTACATTCGCTATAGAAAATGCTATGAGAG
  GAAAAAGCTGCATGTTACCGATATGACAAAAAACATAAAAAACTCTACTTATGAAAGTGT
  TTTGCCCTATAAGAACATATTCAATAAAAGGCATTATTCCAGGTGAATGAGCCATGCGGA
  AATGTGGATTTATTAGGGTTTGTTTTGAAATGTAATTTGTACGGCCAGCTTTTTATTCCT
  TTGATGGCTATCAAATAGATTTATTGGCTACATGTTAGAAGTGCAAAAGATAACAGGTAA
  CCAAGGGAGAAGTTTTAATAAAACTCTCAGGAAAGGCAAAGAAAAGTGAGTGAATATGCC
  TGCTTACATTTCAGCTAATAAATAAAAAGTGCAGATAGGGCTTTAACTGAAAACCTTGCT
  GGTTATTGCAGCCTGGAAGGTTTATTGAAAACTGGGTAGAACACTCTCATTTTTTATATT
  TACAGCTACATTGTGAGCTGTTCAGGTTAGAGTGATGTAACGTTCATTTTGACTTTGTTA
  CAGGAAACTGATGTTTTCACAGGAAACTTGCAAAGCAGTTATGAAGAGGAAGAGCGTTCT
  TAATTATTAGTGTCTGCCTGAGCTATTTCTGATTGGGTTCTGGGATTTTTAGAGAATTGA
  CAATAGAGTATTTAAATTTACCACTCCCCTCTGGAGTAAAACATTAGACCCTTTTAGAAA
  GTTTTAAAAGTACTTTTCAATAACCGAGAACTTTCATTCTAAATAACTGGGAAGATTGCC
  CTGCTTAAGAAAGTTGAACTGGACCTGAAACCCCTTCCTTAAAATCTAAGTTATTTAGGC
  ACTTATTTAACCTGGGACTTTACCACCCTGTACCCCAAACTGGTTTGATTAAGAAACCCC
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033135
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033135.2, NP_149126.1
• RefSeq Size: 3979 bp
• RefSeq ORF: 1095 bp
• Locus ID: 80310
• UniProt ID: Q9GZP0
• Cytogenetics: 11q22.3
• Domains: CUB
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Focal adhesion, Gap junction, Melanoma, Prostate cancer, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.