BCCIP (NM_078469) Human Untagged Clone

CAT#: SC110584

BCCIP (untagged)-Human BRCA2 and CDKN1A interacting protein (BCCIP), transcript variant C

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCCIP
• Synonyms: TOK-1; TOK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_078469, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTCCAGGTCTAAGCGGCGTGCCGTGGAAAGTGGGGTTCCGCAGCCGCCGGATCCCCCAGTCCAGC
  GCGACGAGGAAGAGGAAAAAGAAGTCGAAAATGAGGATGAAGACGATGATGACAGTGACAAGGAAAAGGA
  TGAAGAGGACGAGGTCATTGACGAGGAAGTGAATATTGAATTTGAAGCTTATTCCCTATCAGATAATGAT
  TATGACGGAATTAAGAAATTACTGCAGCAGCTTTTTCTAAAGGCTCCTGTGAACACTGCAGAACTAACAG
  ATCTCTTAATTCAACAGAACCATATTGGGAGTGTGATTAAGCAAACGGATGTTTCAGAAGACAGCAATGA
  TGATATGGATGAAGATGAGGTTTTTGGTTTCATAAGCCTTTTAAATTTAACTGAAAGAAAGGGTACCCAG
  TGTGTTGAACAAATTCAAGAGTTGGTTCTACGCTTCTGTGAGAAGAACTGTGAAAAGAGCATGGTTGAAC
  AGCTGGACAAGTTTTTAAATGACACCACCAAGCCTGTGGGCCTTCTCCTAAGTGAAAGATTCATTAATGT
  CCCTCCACAGATCGCTCTGCCCATGTACCAGCAGCTTCAGAAAGAACTGGCGGGGGCACACAGAACCAAT
  AAGCCATGTGGGAAGTGCTACTTTTACCTTCTGATTAGTAAGACATTTGTGGAAGCAGGAAAAAACAATT
  CCAAAAAGAAACCTAGCAACAAAAAGAAAGCTGCGTTAATGTTTGCAAATGCAGAGGAAGAATTTTTCTA
  TGAGGAGCAGGGAAAACCTGAGGTGCTTGGAGGTCCAGACACAAGAAGAGGATTGGAACCAGTTCCGATA
  CAGCACAATGGTTGGTCTGTTCCCCCAGTATTAGAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_078469 unedited
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAACATGG
  CGTCCAGGTCTAAGCGGCGTGCCGTGGAAAGTGGGGTTCCGCAGCCGCCGGATCCCCCAG
  TCCAGCGCGACGAGGAAGAGGAAAAAGAAGTCGAAAATGAGGATGAAGACGATGATGACA
  GTGACAAGGAAAAGGATGAAGAGGACGAGGTCATTGACGAGGAAGTGAATATTGAATTTG
  AAGCTTATTCCCTATCAGATAATGATTATGACGGAATTAAGAAATTACTGCAGCAGCTTT
  TTCTAAAGGCTCCTGTGAACACTGCAGAACTAACAGATCTCTTAATTCAACAGAACCATA
  TTGGGAGTGTGATTAAGCAAACGGATGTTTCAGAAGACAGCAATGATGATATGGATGAAG
  ATGAGGTTTTTGGTTTCATAAGCCTTTTAAATTTAACTGAAAGAAAGGGTACCCAGTGTG
  TTGAACAAATTCAAGAGTTGGTTCTACGCTTCTGTGAGAAGAACTGTGAAAAGAGCATGG
  TTGAACAGCTGGACAAGTTTTTAAATGACACCACCAAGCCTGTGGGCCTTCTCCTAAGTG
  AAAGATTCATTAATGTCCCTCCACAGATCGCTCTGCCCATGTACCAGCAGCTTCAGAAAG
  AACTGGCGGNGGCACACAGAACCAATAAGCCATGTGGGAAGTGCTACTTTTACCTTCTGA
  TTAGTAAGACATTTGTGGAAGCANGANAAAACAATTCCAAAAAGAAACCTAGNCACANAA
  AGAAAGCTGCGTTAATGTTTGCNAATGCAGAGGAAGAATTTTCTATGAGGAGCAGGNAAA
  CCTGAGTGCTTGGNAGTCCAGACACAGAAGAGGATGGNAACAGTCGATCAGACATGGGTG
  GTCTGTCCCCCATTTAGATAATANACATACAGCAGATGATGAGCCATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_078469 unedited
  CTTGGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTATCTTTATT
  TCTTTATTTGCACTGGGGAAATAACATCTTTGTCTACTATTTCTAGCATCTGAGATCCAG
  GTCAGTTGTGATTAATTTTTCTCTGTATTATGCAATGTATTTTCCTGCTTTTCATGCCTC
  TCTACTGGATGCTAGACATTGTACATTTTACCTTCTTGGGCACTGAATATTTTTGTAGTC
  CCAGAGGTACTTTTGAGCTTTGTCCTAAGATGCAGTGTGATCCTTTCAGGTCCTGCTGTA
  TAGCTGTGTCAGGTGGCAGTGCCCATCTAGGGTTTCTCACTTGTGTTTTCTACCCAGTGC
  CCCATAAATCATGAGGTTTTCCAGCTAGGGTGGTGGGAACAGGCCCTACCCTAGGTCCTG
  TGTCAGCACCACTGTTCCTTCTAATCTTCTCACATGGCTCTTTCTCTGGCCTGAGGTGGT
  TCCTTACACACATGCGCTGATCAGTAGGGCTGTGCTGCCTAGTTGAGGGAGCTCCCTCTG
  GCCCCCGACTTAGATCTGTGTGTCTCTCTCCAGTACTGTGACCGCGAGTTCCGGCCACCT
  TAGTCTCCCTGGATCCCTGGCATTGTTTTCCTACTGCAGGGAGGCTGGCAGGCTCCACCT
  GAGTTCCCCCCGCATGCACACTGCAGCTGGGACCCTCCTCGGAGCATGAGCTGGACATCA
  CAGGGCTCCCCTCCTGAGTTTGCCCTCTCAAGATCACTATCCTTCACTCCCTGGTATTTG
  GTGGCTTGCAAACCGTTGTTTACATATTTTGTCCATTGTTTGCTTGTTTCATGCCAGAGG
  GAAAAGTCCAGTACCTGTTTCTCCATCTTAGCATATGAGACTCTTAATTATTATTTTTTT
  CCTTCCTACCACCAAAAGTTGATGCAAGCATGGAAAACTAAATATCTGGCACCTCGATAT
  GATGGAATCTTCTGATTGGAACTCTGTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_078469
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078469.1, NP_510869.1
• RefSeq Size: 2338 bp
• RefSeq ORF: 879 bp
• Locus ID: 56647
• UniProt ID: Q9P287
• Cytogenetics: 10q26.2
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (C) is alternatively spliced at the 3' end compared to transcript variant A. It encodes an isoform (C) which has the same 284 N-terminal aa, but is truncated at the C-terminus compared to isoform BCCIPalpha.