CD57 (B3GAT1) (NM_018644) Human Untagged Clone

CAT#: SC110387

B3GAT1 (untagged)-Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD57
• Synonyms: CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018644, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGAAGAGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCT
  GGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAAC
  GCCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTGCGCACCGAG
  TACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCACGTGGTGACGCCCACCTACA
  GCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAACACGCTGCTGCACGTGCCCAACCTCCACTG
  GCTGGTGGTGGAGGATGCGCCGCGCCGGACGCCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAAC
  TACACGCACCTGCACGTGGAGACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCC
  CGCGGGGCACCATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCA
  GCCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAGATGCGCAGC
  ACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTACGAGGCCCCACGGGTGAACG
  GGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCCCACCGGCCATTTGCAATAGACATGGCTGG
  ATTTGCCGTCAACCTGCGGCTCATTCTGCAGCGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGA
  GGCTACCAGGAAAGCAGCCTCCTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACT
  GCACCAAGATCCTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTT
  CACTGACCCCTCGGTGGAGATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018644 unedited
  GATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGCATGGTGCTC
  CATTGTTTCTGGAAGAGAGGGATGAGTGTTCCTCTCTGATGAGAATGGAGAGGAGGAGAG
  GAACGGGCTCAGGAGGAGGAGGAATCCGAGAGAGAGCCTGACTGAGGCAGAGAAAGAGGC
  AGTGAGACAAGAGACAAAGGCACGGAGGCGCACTGGCGGACAGCAGAGGGAGGCACACAG
  GCAGAAGTCAGGGCCTCCCTGACTGAGTGGTGTCCACCCCCCAGGGTTCCTGACCCCTGC
  CCCTGGACAGCGACCCCTTCTCAGACTCCAGTTGGGCCGGACTCTCCAAACCTGCTTCCG
  CAATGGGTGGGTTGTGAGTGCTGGTAAGACCTGCTAGCCAACATTCAGCTGCTCTGTCCT
  CTCCATGCCTGGCCGGCCCGGCCCATGCCTGTTCTTTTCTCCCCTGTGCTGCCGCCGCCC
  GTGGCCGCCCCTCTCCTGAACTTACCGCCACTCAGGTAATGAGGAGCCGTGGGTGCAGCC
  AGCCTTGGAGATGCCGAAGAGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTG
  GACTCTGCTCATCACTGTCTGGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAA
  GGATGAGGGCAGTGACCCCCGACGCGAAACGCCGCCCGGCGCCGACCCCAGGGAGTACTG
  CACGTCTGACCGCGACATCGTGGAGGTGGTGCGCACCGAGTACGTGTACACGCGGNCCCC
  GCCATGGTCCGACACGCTGCCCACCATCCACGTGGTGACGCCCACCTACGCCGCCCGGTG
  CAAAGGCCGAGCTGACGCGCATGGNCACACGCTGCTGCACGTGCCAACCCCCTGNNCTGT
  GGTGGAGATGCGCGCGCCGACGCGNTGACGCGCGCCTGTGGCGAACCGGCTCATACCCAC
  CTGACGTGAGACGCCCGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018644 unedited
  NNTTTTAGCTTGGACCGCGGGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAGAGGATTA
  GGACGGTAAAAAACTGTTTTTGATTTTTAATTTTGAAAGCAAATTAAATCCCGTTAAATT
  AAAACAAACCCAATGCCGTTGCCTGGGGGCCCCCCTCACCCCAACCTTTGTTTTTAAAAC
  CCCAAACCCCGGGCCTAACTTTTGGGGGGGGGGGCTTGCCCCCGGGGGAACAGGGAACAA
  ACTGGGTTTGAAAAAAAGGGGGCATTAGGGCCCCTTTTTTGATTTAAAACAAACCCTTTC
  CCAAAAAAAAAAAAGGGGGCAGCCCCCCCGGGGTTTAATTAAAAAAAAAAATCCCCCCCC
  CTAAAAAAGGATTTTAGGGGAAAACAACTGGCCTAACTTTTGCCCAAAAAAACCCCCATT
  TTGTTGTTAATTATAAAAGCCCTGAAAAAAAACCCTAGGTTTTTGGCTAAAAAGGGGGCC
  TAAAAACAACCAAACTGACCTTGGCACTTGGGGGGTTTTTGGGAGGCAAAAAAGGGCCCC
  CACATAAGCCCACCACCTGGGCCCACCCCCTCCTTTTTTTCCCATGCCAACCAACTTGTT
  TCAAAGGGTTTGGTTTCCAAAGGGAAATTCAGGGGGGAAAAAGGTGGGAGAAACCCCCCC
  CAAGGGGGGTCAGGGAATCCGATTCCCCCAAAAGGGCCAAGGAAACTGGGGGGAAATTAA
  ACCCTTCCAAAATAGGCCCCCCCGGCTTATTTTCGAATGTTAGGGTCTGGAAAGGGGCCC
  CCCCCCCCCCCCCCCTGGAGAGGGGTTTAAAAGCCCCTCAACCTCCCTGGAGGATTTATA
  GGGGGGAGGGGTTTTATAAAAAAACACCCTCTCCAGGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018644
• Insert Size: 4860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018644.2, NP_061114.1
• RefSeq Size: 3546 bp
• RefSeq ORF: 1005 bp
• Locus ID: 27087
• UniProt ID: Q9P2W7
• Cytogenetics: 11q25
• Domains: Glyco_transf_43
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein.