CCL14 (NM_004166) Human Untagged Clone

CAT#: SC109751

CCL14 (untagged)-Human chemokine (C-C motif) ligand 14 (CCL14), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCL14
• Synonyms: CC-1; CC-3; CKb1; FLJ16015; HCC-1; HCC-3; MCIF; NCC-2; NCC2; SCYA14; SCYL2; SY14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109751 sequence for NM_004166 edited (data generated by NextGen Sequencing)
  ATGAAGATCTCCGTGGCTGCCATTCCCTTCTTCCTCCTCATCACCATCGCCCTAGGGACC
  AAGACTGAATCCTCCTCACGGGGACCTTACCACCCCTCAGAGTGCTGCTTCACCTACACT
  ACCTACAAGATCCCGCGTCAGCGGATTATGGATTACTATGAGACCAACAGCCAGTGCTCC
  AAGCCCGGAATTGTCTTCATCACCAAAAGGGGCCATTCCGTCTGTACCAACCCCAGTGAC
  AAGTGGGTCCAGGACTATATCAAGGACATGAAGGAGAACTGA
  
  Clone variation with respect to NM_004166.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004166 unedited
  CAATAGGTCATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGCAGGAGCCT
  CTGAAGCTCCCACCAGGCCAGCTCTCCTCCCACACACTTCCCACAGCATGAAGATCTCCG
  TGGCTGCCATTCCCTTCTTCCTCCTCATCACCATCGCCCTAGGGACCAAGACTGAATCCT
  CCTCACGGGGACCTTACCACCCCTCAGAGTGCTGCTTCACCTACACTACCTACAAGATCC
  CGCGTCAGCGGATTATGGATTACTATGAGACCAACAGCCAGTGCTCCAAGCCCGGAATTG
  TCTTCATCACCAAAAGGGGCCATTCCGTCTGTACCAACCCCAGTGACAAGTGGGTCCAGG
  ACTATATCAAGGACATGAACGAGAACTGAGTGACCCAGAAGGGGTGGCGAAGGCACAGCT
  CAGAGACATAAAGAGAAGATGCCAAGGCCCCCTCCTNCACCCACCGCTAACTCTCAGCCC
  CAGTCACCCTCTTGGAGCTTCCCTGCTTTGAATTAAAGACCACCTCATGCTCTTAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCCGGACATAGCTGTTT
  CCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGGCCTTTTCTGGCCCTGGG
  AGCTGCCCCTCCAGTGCCAACCACCTTGGTCCTAAAAAAATAAATCGCTCATTTCTGCTG
  AATAAGAGTCCCTCTACAACATACGGGGGGCAGGGGGCGCATGGAACAAGGGCACGTTGG
  AAGACAACCTGAAGGCCGCGGGTCCAATGGAACCAGCCGGATGCAGGGGCCCAACTGGCT
  ACTGAATCTCGGCCCCGGGTTAAACCAATTCTGCCCCACCCTCCGAGTGTCGGGATCCAG
  CCTGCCTGCCCCCCCATTAATTTTGTTCTTTGTAAAAACGGGTTACCTTATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004166 unedited
  NNNNNNAAAANNTTACTTGNACCGCGCCGCAATCTAGATCGAGTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTAAAAGCATGAGTGGTCTTTAATTCAAAGCAGGAAGCTCCAAAAGGTGA
  CTGGGGCTGAAAGTTACCGGTGGGTGGAGGAGGGGGCCTTGGCATCTTCTCTTTATGTCT
  CTGAGCTGTGCCTTCGCCACCCCTTCTGGGTCACTCAGTTCTCCTTCATGTCCTTGATAT
  AGTCCTGGACCCACTTGTCACTGGGGTTGGTACAGACGGAATGGCCCCTTTTGGTGATGA
  AGACAATTCCGGGCTTGGAGCACTGGCTGTTGGTCTCATAGTAATCCATAATCCGCTGAC
  GCGGGATCTTGTAGGTAGTGTAGGTGAAGCAGCACTCTGAGGGGTGGTAAGGTCCCCGTG
  AGGAGGATTCAGTCTTGGTCCCTAGGGCGATGGTGATGAGGAGGAAGAAGGGAATGGCAG
  CCACGGAGATCTTCATGCTGTGGGAAGCTGTTGTGGGAGGAGAGCTGGCCTGGTGGGAGC
  TTCAGAGGCTCCTGCGGCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACA
  AAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCCTA
  CCGNCCATTTGCGTCAACGGGGGCGGNGTTATTACGACATTTTGGAAAGTCCCGTTGATT
  TTGGTGCCAAAAACAACTCCCATTGACGTCAATGGGGGTGGAGACTTGNAAATCCCCGTG
  AGTCAAACCGCTATCCACGCCCATTNGNTGTACTGCCCAAACCGCATCACCATGGGTTAT
  AGCGATGACTAATACCTTANATGTACTGCCCCAAGTANGAAAGTCCCCGTAAGGTCATGT
  ACTGGGGCATAATGCCAGGCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004166
• Insert Size: 550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004166.3, NP_004157.1
• RefSeq Size: 1344 bp
• RefSeq ORF: 282 bp
• Locus ID: 6358
• Cytogenetics: 17q12
• Domains: IL8
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]
  Transcript Variant: This variant (1) is a bicistronic transcript that includes CCL14 and CCL15. Variant 1 lacks an in-frame exon in the CCL14 coding region, compared to variant 2. Variants 1 and 3 encode isoform 1, which is shorter than isoform 2.