Kv3.2 (KCNC2) (NM_139137) Human Untagged Clone

CAT#: SC109363

KCNC2 (untagged)-Human potassium voltage-gated channel, Shaw-related subfamily, member 2 (KCNC2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Kv3.2
• Synonyms: KV3.2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139137, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAAGATCGAGAACAACGAGAGGGTGATCCTCAATGTCGGGGGCACCCGGCACGAAACCTACCGCA
  GCACCCTCAAGACCCTGCCTGGAACACGCCTGGCCCTTCTTGCCTCCTCCGAGCCCCCAGGCGACTGCTT
  GACCACGGCGGGCGACAAGCTGCAGCCGTCGCCGCCTCCACTGTCGCCGCCGCCGAGAGCGCCCCCGCTG
  TCCCCCGGGCCAGGCGGCTGCTTCGAGGGCGGCGCGGGCAACTGCAGTTCCCGCGGCGGCAGGGCCAGCG
  ACCATCCCGGTGGCGGCCGCGAGTTCTTCTTCGACCGGCACCCGGGCGTCTTCGCCTATGTGCTCAATTA
  CTACCGCACCGGCAAGCTGCACTGCCCCGCAGACGTGTGCGGGCCGCTCTTCGAGGAGGAGCTGGCCTTC
  TGGGGCATCGACGAGACCGACGTGGAGCCCTGCTGCTGGATGACCTACCGGCAGCACCGCGACGCCGAGG
  AGGCGCTGGACATCTTCGAGACCCCCGACCTCATTGGCGGCGACCCCGGCGACGACGAGGACCTGGCGGC
  CAAGAGGCTGGGCATCGAGGACGCGGCGGGGCTCGGGGGCCCCGACGGCAAATCTGGCCGCTGGAGGAGG
  CTGCAGCCCCGCATGTGGGCCCTCTTCGAAGACCCCTACTCGTCCAGAGCCGCCAGGTTTATTGCTTTTG
  CTTCTTTATTCTTCATCCTGGTTTCAATTACAACTTTTTGCCTGGAAACACATGAAGCTTTCAATATTGT
  TAAAAACAAGACAGAACCAGTCATCAATGGCACAAGTGTTGTTCTACAGTATGAAATTGAAACGGATCCT
  GCCTTGACGTATGTAGAAGGAGTGTGTGTGGTGTGGTTTACTTTTGAATTTTTAGTCCGTATTGTTTTTT
  CACCCAATAAACTTGAATTCATCAAAAATCTCTTGAATATCATTGACTTTGTGGCCATCCTACCTTTCTA
  CTTAGAGGTGGGACTCAGTGGGCTGTCATCCAAAGCTGCTAAAGATGTGCTTGGCTTCCTCAGGGTGGTA
  AGGTTTGTGAGGATCCTGAGAATTTTCAAGCTCACCCGCCATTTTGTAGGTCTGAGGGTGCTTGGACATA
  CTCTTCGAGCTAGTACTAATGAATTTTTGCTGCTGATAATTTTCCTGGCTCTAGGAGTTTTGATATTTGC
  TACCATGATCTACTATGCCGAGAGAGTGGGAGCTCAACCTAACGACCCTTCAGCTAGTGAGCACACACAG
  TTCAAAAACATTCCCATTGGGTTCTGGTGGGCTGTAGTGACCATGACTACCCTGGGTTATGGGGATATGT
  ACCCCCAAACATGGTCAGGCATGCTGGTGGGAGCCCTGTGTGCTCTGGCTGGAGTGCTGACAATAGCCAT
  GCCAGTGCCTGTCATTGTCAATAATTTTGGAATGTACTACTCCTTGGCAATGGCAAAGCAGAAACTTCCA
  AGGAAAAGAAAGAAGCACATCCCTCCTGCTCCTCAGGCAAGCTCACCTACTTTTTGCAAGACAGAATTAA
  ATATGGCCTGCAATAGTACACAGAGTGACACATGTCTGGGCAAAGACAATCGACTTCTGGAACATAACAG
  ATCAGTGTTATCAGGTGACGACAGTACAGGAAGTGAGCCGCCACTATCACCCCCAGAAAGGCTCCCCATC
  AGACGCTCTAGTACCAGAGACAAAAACAGAAGAGGGGAAACATGTTTCCTACTGACGACAGGTGATTACA
  CGTGTGCTTCTGATGGAGGGATCAGGAAAGGATATGAAAAATCCCGAAGCTTAAACAACATAGCGGGCTT
  GGCAGGCAATGCTCTGAGGCTCTCTCCAGTAACATCACCCTACAACTCTCCTTGTCCTCTGAGGCGCTCT
  CGATCTCCCATCCCATCTATCTTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139137 unedited
  CTATAGGGCGGCCGCGAATTCGCACGAGGCTCCCCCTAGTCATGTCTGAGTCACAGAGAT
  GGGCAAGATCGAGAACAACGAGAGGGTGATCCTCAATGTCGGGGGCACCCGGCACGAAAC
  CTACCGCAGCACCCTCAAGACCCTGCCTGGAACACGCCTGGCCCTTCTTGCCTCCTCCGA
  GCCCCCAGGCGACTGCTTGACCACGGCGGGCGACAAGCTGCAGCCGTCGCCGCCTCCACT
  GTCGCCGCCGCCGAGAGCGCCCCCGCTGTCCCCCGGGCCAGGCGGCTGCTTCGAGGGCGG
  CGCGGGCAACTGCAGTTCCCGCGGCGGCAGGGCCAGCGACCATCCCGGTGGCGGCCGCGA
  GTTCTTCTTCGACCGGCACCCGGGCGTCTTCGCCTATGTGCTCAATTACTACCGCACCGG
  CAAGCTGCACTGCCCCGCAGACGTGTGCGGGCCGCTCTTCGAGGAGGAGCTGGCCTTCTG
  GGGCATCGACGAGACCGACGTGGAGCCCTGCTGCTGGATGACCTACCGGCAGCACCGCGA
  CGCCGAGGAGGCGCTGGACATCTTCGAGACCCCCGACCTCATTGGCGGCGACCCCGGCGA
  CGACGAGGACCTGGCGGCAAGAGGCTGGCATCGAGCGCCGGGCGGGCTTCNGGGGGGCCC
  CCGACGGCAAATCTGGGCGCTGGAGGGAAGCTGCACCCCGCATGTGGGGCCCTTTTTGAA
  AACCCTACTGGTCCAAGCCGCCAGGTTATAGGGGTTTGGCTTCTTTATTCTTATCCCGGG
  GTTCATTCCAACTTTTTTGCCGGAAAACCTGAAGCTTTAATTTTGGTAAAACAAGAAGAC
  CAGTCTCATGGCCCAAGGGGTGGTCTACGTTGAATGGAAACGGACTGCCTGACATGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139137 unedited
  GGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAAC
  AGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTAAA
  GAGTCTAGAACCAAGCAGCAATTTCTGGCTAAACAATGCAAGCCTGGCTGGCAGTTACCT
  TTCTCTCATGTTTTGTGCCTTCCCCAAGTCATTAAGTAAGAGATCTGGCCTCGGCTTGCG
  TGTAACCAGTAATGACAACCTCTTTGCAGTTATCTGTGTGCAAACAACCAGAGACATTCA
  GAACTCCAATACAGCATTTTTGAAGAAAAGTAAATCAATCAAGAAATTAAACTATTTAAA
  ACATATATTTCTTTCAAATAATAAGAAAAAAATGTCAAGGAGAAAAGTGCCCTCCCTGCC
  CCACAATTCAACATGCAGAACAGTCGACCAATGCTTTCATATCAGCAGGATGGTTCGATG
  CAAGTACACATATCCTGAGCTATCCACAGTCCCCGAACTCTGCAACATTGCTTTCAGGTG
  ATAGAGACAAGATGGTCCATGCAAATGAGCTGACACAAGTCATGTCGTGTGCAATCAAGA
  TAGGATCCCAGACATCTTCAGAATGGTTTACAGTCACAAGAAATAAAGTTCCAATGAAGT
  GGTTGGCATTTGGAAGCACACTGTTTTAAATATATCTCCCTGAAGGTATGTTTATATATT
  AGTGCACTGGTCAATATCTGACACTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139137
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139137.2, NP_631875.1
• RefSeq Size: 5512 bp
• RefSeq ORF: 1917 bp
• Locus ID: 3747
• UniProt ID: Q96PR1
• Cytogenetics: 12q21.1
• Domains: BTB, K_tetra, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (2) encodes the longest isoform (KV3.2b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.