IL15RA (NM_002189) Human Untagged Clone

CAT#: SC109344

IL15RA (untagged)-Human interleukin 15 receptor, alpha (IL15RA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL15RA
• Synonyms: CD215
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109344 sequence for NM_002189 edited (data generated by NextGen Sequencing)
  ATGGCCCCGCGGCGGGCGCGCGGCTGCCGGACCCTCGGTCTCCCGGCGCTGCTACTGCTG
  CTGCTGCTCCGGCCGCCGGCGACGCGGGGCATCACGTGCCCTCCCCCCATGTCCGTGGAA
  CACGCAGACATCTGGGTCAAGAGCTACAGCTTGTACTCCAGGGAGCGGTACATTTGTAAC
  TCTGGTTTCAAGCGTAAAGCCGGCACGTCCAGCCTGACGGAGTGCGTGTTGAACAAGGCC
  ACGAATGTCGCCCACTGGACAACCCCCAGTCTCAAATGCATTAGAGACCCTGCCCTGGTT
  CACCAAAGGCCAGCGCCACCCTCCACAGTAACGACGGCAGGGGTGACCCCACAGCCAGAG
  AGCCTCTCCCCTTCTGGAAAAGAGCCCGCAGCTTCATCTCCCAGCTCAAACAACACAGCG
  GCCACAACAGCAGCTATTGTCCCGGGCTCCCAGCTGATGCCTTCAAAATCACCTTCCACA
  GGAACCACAGAGATAAGCAGTCATGAGTCCTCCCACGGCACCCCCTCTCAGACAACAGCC
  AAGACCTGGGAACTCACAGCATCCGCCTCCCACCAGCCGCCAGGTGTGTATCCACAGGGC
  CACAGCGACACCACTGTGGCTATCTCCACGTCCACTGTCCTGCTGTGTGGGCTGAGCGCT
  GTGTCTCTCCTGGCATGCTACCTCAAGTCAAGGCAAACTCCCCCGCTGGCCAGCGTTGAA
  ATGGAAGCCATGGAGGCTCTGCCGGTGACTTGGGGGACCAGCAGCAGAGATGAAGACTTG
  GAAAACTGCTCTCACCACCTATGA
  
  Clone variation with respect to NM_002189.3
  545 a=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002189 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGGGAGTCCAGC
  GGTGTCCTGTGGAGCTGCCGCCATGGCCCCGCGGCGGGCGCGCGGCTGCCGGACCCTCGG
  TCTCCCGGCGCTGCTACTGCTGCTGCTGCTCCGGCCGCCGGCGACGCGGGGCATCACGTG
  CCCTCCCCCCATGTCCGTGGAACACGCAGACATCTGGGTCAAGAGCTACAGCTTGTACTC
  CAGGGACCGGTACATTTGTAACTCTGGTTTCAAGCGTAAAGCCGGCACGTCCAGCCTGAC
  GGAGTGCGTGTTGAACAAGGCCACGAATGTCGCCCACTGGACAACCCCCAGTCTCAAATG
  CATTAGAGACCCTGCCCTGGTTCACCAAAGGCCAGCGCCACCCTCCACAGTAACGACGGC
  AGGGGTGACCCCACAGCCAGAGAGCCTCTCCCCTTCTGGAAAAGAGCCCGCAGCTTCATC
  TCCCAGCTCAAACAACACAGCGGCCACAACAGCAGCTATTGTCCCGGGCTCCCAGCTGAT
  GCCTTCAAAATCACCTTCCACAGGAACCACAGAGATAAGCAGTCATGAGTCCTCCCACGG
  CACCCCCTCTCAGACAACAGCCAAGACCTGNGAACTCACAGCATCCGCCTCCCACCAGCC
  GCCAGGTGTGTATCCACAGGGCCACAGCGACACCACTGTGGCTATCTCCACGTCCACTGT
  CCTGCTGTGTGGGCTGAGCGCTGTGTCTCTCCTGGCATGCTACCTCAAGTCAAGGCANAC
  TCCCCCCGCTGCCAGCGTTGAAATGGAAGCCATGGNAGCTCTGNNCGTGACTTGNGGGAC
  CAGCAGCAGAGATGAAGACTTGGAANACTGCTCTCACACCTATGAAACTCGNGAAACCAG
  NCCAGCTAGTCCGNAGTGAGGAGCTCTCTGCTTTACTAAGACACTGAAAGAGTGCAGGAA
  GCGGNCTCAGACAGCTACCAGCNTAAAGTCCACAGATTACGACTGTCGGTCGGCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002189 unedited
  AAGGAGGCGCCTAGGNGAATGGCACTTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCAC
  AGGATGCCACCCGGGAATTCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAG
  TCGAGTTTTTTTTTTTTTTTTTTTATTGAAAATAGATGTTTTATTTTGTTTATACAAGGT
  ACAATGTCAAAATACAAATAATATATAATGTATAGATATAATAGACAAGGAAGTATAAAT
  ATAAACGCATATATTCGTAAAATGGCACTGAGTTGAGTTTTCTTCTTCCTGAATCCTTCA
  ATGGAGAGGATTCGCTGGGCTCAGCATCTCTCCCACCTTTCCCAGGTCCCTGTCCATGTG
  TGCAGAGCAGCTGGAGACAGGGTGGTTAGAAGCCCAAACGCTGGTGTCTTCCCTGTAGAC
  GTCTCCCACGCCAGGAGAAGCCTTGTAATTGACAGAGAGCTTTGGGTATGTCACTTTTCT
  CTGTGAACTGCAAGTTAGGATGAGGGACGGAAGATTCGGGGCAGGGTTTTCATTTCATCA
  CCACATGTATTCCAAGCAGCTCACACTGTAATGAAATAAAAATCCTGCTCAGAAGCCTTT
  GGTCTCTCCTGGGAAGCTGGGCCCTGGGTCCAAGGCACGACAGGCAGGCAAGTGGTGCCC
  ATGGGAAAGCGGAGAACCTGCTCCTTCGCGCAGGAAGCGCCGAACCCGCAGTCCGGGAAT
  CCTGCTGGAATTTTAAAAAGCCTGGTGAACTTGTTCCTGAAACCCGCCTCATTGCAACTA
  TTTCTAATACACTTTTACTAAAGCAAAAAAGCTCCTTTTTTCCCAAATTATCTTGGATGG
  CTTCACCAAATTTTAAAATTGTGGAGAACATTTTCCACAGTCTCACTTGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002189
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002189.2, NP_002180.1
• RefSeq Size: 1610 bp
• RefSeq ORF: 804 bp
• Locus ID: 3601
• UniProt ID: Q13261
• Cytogenetics: 10p15.1
• Domains: CCP
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.[provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (1) lacks an alternate in-frame 5' coding exon, compared to variant 4. This results in a shorter isoform (1), compared to isoform 4.