Eph receptor B2 (EPHB2) (NM_017449) Human Untagged Clone

CAT#: SC109198

EPHB2 (untagged)-Human EPH receptor B2 (EPHB2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Eph receptor B2
• Synonyms: BDPLT22; CAPB; DRT; EK5; EPHT3; ERK; Hek5; PCBC; Tyro5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_017449 edited
  ATGGCTCTGCGGAGGCTGGGGGCCGCGCTGCTGCTGCTGCCGCTGCTCGCCGCCGTGGAA
  GAAACGCTAATGGACTCCACTACAGCGACTGCTGAGCTGGGCTGGATGGTGCATCCTCCA
  TCAGGGTGGGAAGAGGTGAGTGGCTACGATGAGAACATGAACACGATCCGCACGTACCAG
  GTGTGCAACGTGTTTGAGTCAAGCCAGAACAACTGGCTACGGACCAAGTTTATCCGGCGC
  CGTGGCGCCCACCGCATCCACGTGGAGATGAAGTTTTCGGTGCGTGACTGCAGCAGCATC
  CCCAGCGTGCCTGGCTCCTGCAAGGAGACCTTCAACCTCTATTACTATGAGGCTGACTTT
  GACTCGGCCACCAAGACCTTCCCCAACTGGATGGAGAATCCATGGGTGAAGGTGGATACC
  ATTGCAGCCGACGAGAGCTTCTCCCAGGTGGACCTGGGTGGCCGCGTCATGAAAATCAAC
  ACCGAGGTGCGGAGCTTCGGACCTGTGTCCCGCAGCGGCTTCTACCTGGCCTTCCAGGAC
  TATGGCGGCTGCATGTCCCTCATCGCCGTGCGTGTCTTCTACCGCAAGTGCCCCCGCATC
  ATCCAGAATGGCGCCATCTTCCAGGAAACCCTGTCGGGGGCTGAGAGCACATCGCTGGTG
  GCTGCCCGGGGCAGCTGCATCGCCAATGCGGAAGAGGTGGATGTACCCATCAAGCTCTAC
  TGTAACGGGGACGGCGAGTGGCTGGTGCCCATCGGGCGCTGCATGTGCAAAGCAGGCTTC
  GAGGCCGTTGAGAATGGCACCGTCTGCCGAGGTTGTCCATCTGGGACTTTCAAGGCCAAC
  CAAGGGGATGAGGCCTGTACCCACTGTCCCATCAACAGCCGGACCACTTCTGAAGGGGCC
  ACCAACTGTGTCTGCCGCAATGGCTACTACAGAGCAGACCTGGACCCCCTGGACATGCCC
  TGCACAACCATCCCCTCCGCGCCCCAGGCTGTGATTTCCAGTGTCAATGAGACCTCCCTC
  ATGCTGGAGTGGACCCCTCCCCGCGACTCCGGAGGCCGAGAGGACCTCGTCTACAACATC
  ATCTGCAAGAGCTGTGGCTCGGGCCGGGGTGCCTGCACCCGCTGCGGGGACAATGTACAG
  TACGCACCACGCCAGCTAGGCCTGACCGAGCCACGCATTTACATCAGTGACCTGCTGGCC
  CACACCCAGTACACCTTCGAGATCCAGGCTGTGAACGGCGTTACTGACCAGAGCCCCTTC
  TCGCCTCAGTTCGCCTCTGTGAACATCACCACCAACCAGGCAGCTCCATCGGCAGTGTCC
  ATCATGCATCAGGTGAGCCGCACCGTGGACAGCATTACCCTGTCGTGGTCCCAGCCGGAC
  CAGCCCAATGGCGTGATCCTGGACTATGAGCTGCAGTACTATGAGAAGGAGCTCAGTGAG
  TACAACGCCACAGCCATAAAAAGCCCCACCAACACGGTCACCGTGCAGGGCCTCAAAGCC
  GGCGCCATCTATGTCTTCCAGGTGCGGGCACGCACCGTGGCAGGCTACGGGCGCTACAGC
  GGCAAGATGTACTTCCAGACCATGACAGAAGCCGAGTACCAGACAAGCATCCAGGAGAAG
  TTGCCACTCATCATCGGCTCCTCGGCCGCTGGCCTGGTCTTCCTCATTGCTGTGGTTGTC
  ATCGCCATCGTGTGTAACAGACGGGGGTTTGAGCGTGCTGACTCGGAGTACACGGACAAG
  CTGCAACACTACACCAGTGGCCACATGACCCCAGGCATGAAGATCTACATCGATCCTTTC
  ACCTACGAGGACCCCAACGAGGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGT
  GTCAAAATTGAGCAGGTGATCGGAGCAGGGGAGTTTGGCGAGGTCTGCAGTGGCCACCTG
  AAGCTGCCAGGCAAGAGAGAGATCTTTGTGGCCATCAAGACGCTCAAGTCGGGCTACACG
  GAGAAGCAGCGCCGGGACTTCCTGAGCGAAGCCTCCATCATGGGCCAGTTCGACCATCCC
  AACGTCATCCACCTGGAGGGTGTCGTGACCAAGAGCACACCTGTGATGATCATCACCGAG
  TTCATGGAGAATGGCTCCCTGGACTCCTTTCTCCGGCAAAACGATGGGCAGTTCACAGTC
  ATCCAGCTGGTGGGCATGCTTCGGGGCATCGCAGCTGGCATGAAGTACCTGGCAGACATG
  AACTATGTTCACCGTGACCTGGCTGCCCGCAACATCCTCGTCAACAGCAACCTGGTCTGC
  AAGGTGTCGGACTTTGGGCTCTCACGCTTTCTAGAGGACGATACCTCAGACCCCACCTAC
  ACCAGTGCCCTGGGCGGAAAGATCCCCATCCGCTGGACAGCCCCGGAAGCCATCCAGTAC
  CGGAAGTTCACCTCGGCCAGTGATGTGTGGAGCTACGGCATTGTCATGTGGGAGGTGATG
  TCCTATGGGGAGCGGCCCTACTGGGACATGACCAACCAGGATGTAATCAATGCCATTGAG
  CAGGACTATCGGCTGCCACCGCCCATGGACTGCCCGAGCGCCCTGCACCAACTCATGCTG
  GACTGTTGGCAGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAAATTGTCAACACGCTA
  GACAAGATGATCCGCAATCCCAACAGCCTCAAAGCCATGGCGCCCCTCTCCTCTGGCATC
  AACCTGCCGCTGCTGGACCGCACGATCCCCGACTACACCAGCTTTAACACGGTGGACGAG
  TGGCTGGAGGCCATCAAGATGGGGCAGTACAAGGAGAGCTTCGCCAATGCCGGCTTCACC
  TCCTTTGACGTCGTGTCTCAGATGATGATGGAGGACATTCTCCGGGTTGGGGTCACTTTG
  GCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCGCAGATGAACCAG
  ATTCAGTCTGTGGAGGTTTGACATTCACCTGCCTCGGCTCACCTCTTCCTCCAAGCCCCG
  CCCCCTCTGCCCCACGTGCCGGCCCTCCTGGTGCTCTATCCACTGCAGGGCCAGCCACTC
  GCCAGGAGGCCACGGGCCACGGGAAGAACCAAGCGGTGCCAGCCACGAGACGTCACCAAG
  AAAACATGCAACTCAAACGACGGAAAAAAAAAGGGAATGGGAAAAAAGAAAACAGATCCT
  GGGAGGGGGCGGGAAATACAAGGAATATTTTTTAAAGAGGATTCTCATAAGGAAAGCAAT
  GACTGTTCTTGCGGGGGATAAAAAAGGGCTTGGGAGATTCATGCGATGTGTCCAATCGGA
  GACAAAAGCAGTTTCTCTCCAACTCCCTCTGGGAAGGTGACCTGGCCAGAGCCAAGAAAC
  ACTTTCAGAAAAACAAATGTGAAGGGGAGAGACAGGGGCCGCCCTTGGCTCCTGTCCCTG
  CTGCTCCTCTAGGCCTCACTCAACAACCAAGCGCCTGGAGGACGGGACAGATGGACAGAC
  AGCCACCCTGAGAACCCCTCTGGGAAAATCTATTCCTGCCACCACTGGGCAAACAGAAGA
  ATTTTTCTGTCTTTGGAGAGTATTTTAGAAACTCCAATGAAAGACACTGTTTCTCCTGTT
  GGCTCACAGGGCTGAAAGGGGCTTTTGTCCTCCTGGGTCAGGGAGAACGCGGGGACCCCA
  GAAAGGTCAGCCTTCCTGAGGATGGGCAACCCCCAGGTCTGCAGCTCCAGGTACATATCA
  CGCGCACAGCCTGGCAGCCTGGCCCTCCTGGTGCCCACTCCCGCCAGCCCCTGCCTCGAG
  GACTGATACTGCAGTGACTGCCGTCAGCTCCGACTGCCGCTGAGAAGGGTTGATCCTGCA
  TCTGGGTTTGTTTACAGCAATTCCTGGACTCGGGGGTATTTTGGTCACAGGGTGGTTTTG
  GTTTAGGGGGTTTGTTTGTTGGGTTGTTTTTTGTTTTTTGGTTTTTTTTAATGACAATGA
  AGTGACACTTTGACATTAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC
  GGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017449 unedited
  CGCCTTGCCGCCCCCCCTGGCCCCCCGAGCCCGGGGCGCGCGCTCCCGCCCGGGCCGTCC
  GGGCCCCGCGGCGCCGCGGCCCGAGGCCCCGGGAAGCGCAGCCATGGCTCTGCGGAGGCT
  GGGGGCCGCGCTGCTGCTGCTGCCGCTGCTCGCCGCCGTGGAAGAAACGCTAATGGACTC
  CACTACAGCGACTGCTGAGCTGGGCTGGATGGTGCATCCTCCATCAGGGTGGGAAGAGGT
  GAGTGGCTACGATGAGAACATGAACACGATCCGCACGTACCAGGTGTGCAACGTGTTTGA
  GTCAAGCCAGAACAACTGGCTACGGACCAAGTTTATCCGGCGCCGTGGCGCCCACCGCAT
  CCACGTGGAGATGAAGTTTTCGGTGCGTGACTGCAGCAGCATCCCCAGCGTGCCTGGCTC
  CTGCAAGGAGACCTTCAACCTCTATTACTATGAGGCTGACTTTGACTCGGCCACCAAGAC
  CTTCCCCAACTGGATGGAGAATCCATGGGTGAAGGTGGATACCATTGCAGCCGACGAGAG
  CTTCTCCCAGGTGGACCTGGGTGGCCGCGTCATGAAAATCAACACCGAGGTGCGGAGCTT
  CGGACCTGTGTCCCGCAGCGGCTTCTACCTGGCCTTCCAGGACTATTGGCGGCTGCATGT
  CCCTCATCGCCCGTGCGTGTCTTCTACCGCAAGTGGCCCCGCATCATCCAGAATGGCGCC
  ATCTTNCAGAAACCCCTGGCGGGGGGCTGAGACACATNGCTTGGTGGCTGCCCGGGGCAA
  CTGCATCGCCATGCGGAAGAGAGAGGGGAGTACCCCTCAAGCTCTACTGTACCGGGGACC
  GCCAAGGGGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017449 unedited
  TAAGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTAATGTCAA
  AGTGTCACTTCATTGTCATTAAAAAAAACCAAAAACAAAAAACAACCCACAAACAAACCC
  CCTAAACCAAAACCACCCTGTGACCAAAATACCCCCGAGTCCAGGAATTGCTGTAAACAA
  ACCCAGATGCAGGATCAACCCTTCTCAGCGGCAGTCGGAGCTGACGGCAGTCACTGCAGT
  ATCAGTCCTCGAGGCAGGGGCTGGCGGGAGTGGGCACCAGGAGGGCCAGGCTGCCAGGCT
  GTGCGCGTGATATGTACCTGGAGCTGCAGACCTGGGGGTTGCCCATCCTCAGGAAGGCTG
  ACCTTTCTGGGGTCCCCGCGTTCTCCCTGACCCAGGAGGACAAAAGCCCCTTTCAGCCCT
  GTGAGCCAACAGGAGAAACAGTGTCTTTCATTGGAGTTTCTAAAATACTCTCCAAAGACA
  GAAAAATTCTTCTGTTTGCCCAGTGGTGGCAGGAATAGATTTTCCCAGAGGGGTTCTCAG
  GGTGGCTGTCTGTCCATCTGTCCCGTCCTCCAGGCGCTTGGTTGTTGAGTGAGGCCTANA
  GGAGCAGCAGGGACAGGAGCCAAGGGCGGCCCCTGTCTCTCCCCTTCACATTTGTTTTTC
  TGAAAGTGTTTCTTGGCTCTGGCCAGGTCACCTTCCCAGAGGGAGTTGGAGAGAAACTGC
  TTTTGTCTCCGATTGGACACATCGCATGAATCTCCCAAGCCCTTTTTTATCCCCCGCGAG
  AACAGTCATTGCTTTCCCTAATGAAATCCTCTTTAAAAAAATTCCTTGTATTTCCCCGCC
  CCTCCCAGAATCTCGTTTCCTTTTTTCCCATTCCCTTTTTTTTTCCGTCGTTTGGAATGC
  ATGTTATCTTGGGACTTCTCGGGGCTGCACCGCTTTTTCTTCCCTGGCCCAGGCCCTCCG
  GCGATGGCGGGCCCCGCGTGGAAAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017449
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017449.2, NP_059145.1
• RefSeq Size: 4866 bp
• RefSeq ORF: 3168 bp
• Locus ID: 2048
• UniProt ID: P29323
• Cytogenetics: 1p36.12
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site compared to variant 2. It encodes isoform 1, which is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. CCDS Note: The coding region has been updated to represent an alternative 3' splice pattern, resulting in a shorter and distinct C-terminus that is better supported by available transcript and homology data.