TTC8 (NM_144596) Human Untagged Clone

CAT#: SC108831

TTC8 (untagged)-Human tetratricopeptide repeat domain 8 (TTC8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TTC8
• Synonyms: BBS8; RP51
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_144596, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCTCGGAGATGGAGCCGCTGCTCCTGGCCTGGAGCTATTTTAGGCGCAGGAAGTTCCAGCTCTGCG
  CCGATCTATGCACGCAGATGCTGGAGAAGTCCCCTTATGACCAGGAACCAGATCCTGAATTGCCAGTGCA
  TCAGGCAGCTTGGATCTTAAAAGCAAGAGCGCTAACAGAAATGGTATACATAGATGAAATTGATGTAGAT
  CAGGAAGGAATTGCAGAAATGATGCTGGATGAAAATGCTATAGCTCAAGTTCCACGCCCTGGAACGTCTT
  TGAAACTCCCTGGAACTAATCAGACAGGAGGGCCTAGCCAGGCCGTTAGGCCAATCACACAAGCTGGAAG
  ACCCATTACAGGTTTCCTCAGGCCCAGCACGCAGAGTGGAAGGCCAGGCACTATGGAACAGGCTATCAGA
  ACACCCAGAACCGCCTACACAGCCCGCCCTATCACCAGCTCCTCCGGAAGATTTGTCAGGCTGGGAACGG
  CTTCCATGCTTACAAGTCCTGATGGACCATTTATAAATTTATCTAGGCTGAATTTAACAAAGTATTCCCA
  GAAACCTAAGTTGGCAAAGGCTTTGTTTGAGTATATCTTTCATCATGAAAATGATGTTAAGACTGCTTTG
  GATCTGGCTGCCCTCTCCACAGAACATTCTCAGTACAAGGACTGGTGGTGGAAAGTACAGATTGGAAAAT
  GTTACTACAGGTTGGGAATGTATCGTGAAGCAGAAAAACAGTTTAAATCAGCCCTGAAGCAGCAGGAAAT
  GGTAGATACATTTCTGTACTTGGCAAAAGTTTATGTCTCATTGGATCAACCTGTGACTGCTTTAAATCTT
  TTCAAACAAGGCTTAGATAAGTTTCCAGGAGAAGTAACCCTGCTCTGTGGAATTGCAAGAATCTATGAGG
  AAATGAACAATATGTCATCAGCAGCAGAATATTACAAAGAAGTTTTGAAACAAGACAATACTCATGTGGA
  AGCCATCGCATGCATTGGAAGCAACCACTTCTATTCTGATCAGCCAGAAATAGCTCTCCGGTTTTACAGG
  CGGCTGCTGCAGATGGGCATTTATAACGGCCAGCTTTTTAACAATCTGGGGCTGTGTTGCTTCTATGCCC
  AGCAGTATGATATGACTCTGACCTCATTTGAACGTGCCCTTTCTTTGGCTGAAAATGAAGAAGAGGCAGC
  TGATGTCTGGTACAACTTGGGACATGTAGCTGTGGGAATAGGAGATACAAATTTGGCCCATCAGTGCTTC
  AGGCTGGCTCTGGTCAACAACAACAACCACGCCGAGGCCTACAACAACCTGGCTGTGCTGGAGATGCGGA
  AGGGCCACGTTGAACAGGCAAGGGCACTATTACAAACTGCATCATCATTAGCACCCCATATGTATGAACC
  GCATTTTAATTTTGCAACAATCTCTGATAAGATTGGAGATCTGCAGAGAAGCTATGTTGCTGCGCAGAAG
  TCTGAAGCAGCATTTCCAGACCATGTGGACACACAACATTTAATTAAACAATTAAGGCAGCATTTTGCTA
  TGCTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144596 unedited
  NAGGTGTGCCCTATTTAGTTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCA
  GAGTCGGACGCCGCCAGCTCTTCACTCCACGCCCACCTCTCTCCTGGAGCGCTGGGCCTT
  CGCTGGCCGCACCGGCAGCCATGAGCTCGGAGATGGAGCCGCTGCTCCTGGCCTGGAGCT
  ATTTTAGGCGCAGGAAGTTCCAGCTCTGCGCCGATCTATGCACGCAGATGCTGGAGAAGT
  CCCCTTATGACCAGGCAGCTTGGATCTTAAAAGCAAGAGCGCTAACAGAAATGGTATACA
  TAGATGAAATTGATGTAGATCAGGAAGGAATTGCAGAAATGATGCTGGATGAAAATGCTA
  TAGCTCAAGTTCCACGCCCTGGAACGTCTTTGAAACTCCCTGGAACTAATCAGACAGGAG
  GGCCTAGCCAGGCCGTTAGGCCAATCACACAAGCTGGAAGACCCATTACAGGTTTCCTCA
  GGCCCAGCACGCAGAGTGGAAGGCCAGGCACTATGGAACAGGCTATCAGAACACCCAGAA
  CCGCCTACACAGCCCGCCCTATCACCAGCTCCTCCGGAAGATTTGTCAGGCTGGGAACGG
  CTTCCATGCTTACAAGTCCTGATGGACCATTTATAAATTTATCTAGGCTGAATNTAACAA
  AGTATTCCCAGAAACCTAAGTTGGCAAAGGCTNTGTTTGAGTATATCTTTCATCATGAAA
  ATGATGTTAAGACTGCTTTGGATCTGGCTGCCCTCTCCACAGACATTCTCAGTACAAGGA
  CTGGTGGTGGAAAGTACAGATTGGAAAATGTTACTACAGGTTGGGAATGTATCGTGAAGC
  AGAAAACAGTTTAAATCAGCCCTGAAGCAGCNAGAAATGGTAGATACATTTCTGTACTGG
  GCAAAAGTTATGTCTCATTGGATCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_144596 unedited
  ATTTACTGTGNACCGCGCGCCGCTTNCTAGNGATCGATTTTTTTTTTTTTTTTTTTGATG
  GTGTTTTACACAATGATTTATTTTAAAGCTTAATAGGAAATCACAAAGTATTTAATAAAA
  AATAGTTTTAAATACATTTTTAAAGTCTTAAGGAAAGTCTCTATTTATAATCCTCCCACC
  TCCACCTCCCAAGTAGCTGGGACTATAGGCACATGCTACCACACCTGGCTTAAAGGTGCA
  GTTTTAATGGCAGATTTCAGACTCCTTAAGCTACATGATGGCTAAGAGTCAGCTTAAGGA
  CTTAAAAAATGTACATTTATAAAACATTGAGGAAATTCACCAAAATGTTTTGTAAACATT
  ATAAACAAACATCTACTATATAATGTGAGAGACAGTTTTAGGATCTATTTAGAAAGGTTT
  AAATCCTGTATTTTATAATATTATTTTATAAAGTTACTATTACTATGAAATTTGCATTAC
  ACATTCTGTGTCACCCTTATGTGTCACCTTAACTAATATCAAGGACAGGTTTGTTAAAAT
  ATACGTATTACACTATATACATACATACACAGACATAGTGCTTTTTTCCCCTTGCATAAT
  GCTGCTTCATAAGAACATATGTGGTCTAAGGAACAATCAGAGCATAGCAAAATGCTGCCT
  TAATTGTTTAAATAAATGTTGTGTGTCCACATGGTCTGGAAATGCTGCTTCAAACTTCTG
  CGCACCAACATAACTTTCTCTGCAGATCTCCAATCCTTATCAGAGATTGTTGCGAAAATA
  AAATGCGGTTCATACATAATGGGGTGCTAAAGATGAAGCAGTTTGTAAAAATGCCCCTTG
  CCCGTTCACAGTGGCCCTTCCGCATCTCCACACAACCAGTTGTTGTAAGCCTCCGCCTGG
  TTTTTGTTGTTACCAAACCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_144596
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144596.2, NP_653197.2
• RefSeq Size: 2248 bp
• RefSeq ORF: 1548 bp
• Locus ID: 123016
• UniProt ID: Q8TAM2
• Cytogenetics: 14q31.3
• Domains: TPR
• Gene Summary: This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is involved in the formation of cilia. A mutation in this gene has also been implicated in nonsyndromic retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (1) encodes isoform A.