RAMAC (NM_031452) Human Untagged Clone

CAT#: SC108758

FAM103A1 (untagged)-Human family with sequence similarity 103, member A1 (FAM103A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAMAC
• Synonyms: C15orf18; FAM103A1; HsT19360; RAM; RAMMET
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108758 sequence for NM_031452 edited (data generated by NextGen Sequencing)
  ATGACTGACACTGCCGAAGCTGTTCCAAAGTTTGAAGAGATGTTTGCTAGTAGATTCACA
  GAAAATGACAAGGAGTATCAGGAATACCTGAAACGCCCTCCTGAGTCTCCTCCAATTGTT
  GAGGAATGGAATAGCAGAGCTGGTGGGAACCAAAGAAACAGAGGCAATCGGTTGCAAGAC
  AACAGACAGTTCAGAGGCAGGGACAACAGATGGGGGTGGCCAAGTGACAATCGATCCAAT
  CAGTGGCATGGACGATCCTGGGGTAACAACTACCCGCAACACAGACAAGAACCTTACTAT
  CCCCAGCAATATGGACATTATGGTTACAACCAGCGGCCTCCTTACGGTTACTACTGA
  
  Clone variation with respect to NM_031452.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031452 unedited
  TCAAATATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGNAGAGAGA
  GAGGCGCTCGGTTACATTGGAGAACTGGAGTGGTCTGGAGTTCCACGGTGTAGTGGACCA
  GAGGCCACCTCTCCTGGGCTTCTCAGTGTCTCGCCGGCGGGGTTCGGCCTGAGCTGGATT
  GACATAGCCCTTGGCGGATTTAAACAACCTAAACATTAAGCAGTACAGCTGCCTCAAACC
  TTTGGGATTTTCAGAATGACTGACACTGCCNAAGCTGTTCCAAAGTTTGAAGAGATGTTT
  GCTAGTAGATTCACAGAAAATGACAAGGAGTATCAGGAATACCTGAAACGCCCTCCTGAG
  TCTCCTCCAATTGTTGAGGAATGGAATAGCAGAGCTGGTGGGAACCAAAGAAACAGAGGC
  AATCGGTTGCAAGACAACAGACAGTTCAGAGGCAGGGACAACAGATGGGGGTGGCCAAGT
  GACAATCGATCCAATCAGTGGCATGGACGATCCTGGGGTAACAACTACCCGCAACACAGA
  CAAGAACCTTACTATCCCCAGCAATATGGACATTATGGTTACAACCAGCGGCCTCCTTAC
  GGTTACTACTGATAGAAATGTTGGCAGCTTTTAGTAAAAGCATTTACTCTGTTACCATGA
  GAAAGTNTGGGGTGTCTTCTGTTGGTCATAGTTTTACATCTGATTTACAGAATGGATTAT
  TGATTTTTTGGGAAAGTGAGACTNTAAAAAAATAGATCTTACTTGCGAATGCCGATGGTT
  GCTGGGGATACCTGANACTGTGGATATAATTGCTGGACTTCTACCTCAGAATCTNCTTGG
  TTCATGACTTATATGGCTCTNTAAGGTTGGGATAATATTTGACCTCTAGAATTCTTTGTT
  TTCACAGAATTAAAATTTAAATAAAAAGCTTTACTTTGTAGGGNAGAAGATCATGCCCCT
  AAGGGGCCGTTCTAATATAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031452 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTATTTTACAGCTTTATTACTGAT
  TTCCCTCTAAAAAGAGACTCAAGTGTGGAGCTGACTCATCTATAGATTAAGGAATCACAA
  AGTACCATAGTCACTTAACAAATGCAAAAAAAAAAAAAAGCAATTGTTTTACCTTTCAAC
  TGCTGATGTTATGTACAATCTATCACAAAAAGCATAGCTGTGCATTCATGAGCTGATTAT
  ATCAACCTCTAGAATTTTAGAAACGAGCACATCTAAGCATATGGATACTCTCTTACCTTA
  CAAAGTAAAAGCATTTTCTATTTTAAAATTTTTATTTCTGTGTAAAACAAAGAATTCCTA
  GAGGTCAAATAATATACCAAACTTAAAGCACTATTAAGTCATGAAACAAAGAAGATTCTG
  AGGTAGAAGTCAAGCAATATAATCCACAGTTTCAGGTATTCCCAGCAACCATCGCATTTC
  GCAAGTAAGATCTATTNTTTTTAAAGTCTCAACTTCCAAAAATCAATAATCCATTCTGTA
  AAATCAGATGTAAAACTATGACCAACAGAAGACACCCAAACTTTTCTCATGGGTACAGAG
  TAAATGCTTTTACTAAAAGCTGCCCACATTTCTATCAGTAGTACCCGTAAGGAGGCCGCT
  GGNTGTAACCATAATGTCCATATTGCTGGGGATAGTAAGGNTCTTGTCTGTNGTGCGGGT
  ANTTGNTACCCCCAGATCGTCCATGCCACTGATGGNATCGNATGCCACTTGCCCACCCCC
  ATCTGNTGTCCNTGCCTCTGAACTGNCTGTTGTCTTGCACCCGATGCCTTTTGTTTCTTT
  GGTCCCACCAGCTCTGCTTTTCATCCCTCACAATTGGAGAAACTCAGAGGNCGTTCAGAA
  ATCCTGAACCTCCTGCATTTCTTGGATCTACTACAAACATTTTTAACTGNGAACAGNNTC
  GCAGGTCAGTCATCTGAAATCCNAANGTTTGAGCACCTGCCGCTTATGGTTTAGTTGTTA
  ATCCGCCAGGCTATGTAATCAACTAGGCCGACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031452
• Insert Size: 1240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031452.2, NP_113640.1
• RefSeq Size: 1220 bp
• RefSeq ORF: 357 bp
• Locus ID: 83640
• UniProt ID: Q9BTL3
• Cytogenetics: 15q25.2
• Gene Summary: Regulatory subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs (PubMed:22099306, PubMed:27422871). Promotes the recruitment of the methyl donor, S-adenosyl-L-methionine, to RNMT (PubMed:27422871). Regulates RNMT expression by a post-transcriptional stabilizing mechanism (PubMed:22099306). Binds RNA (PubMed:22099306).[UniProtKB/Swiss-Prot Function]