LSM7 (NM_016199) Human Untagged Clone

CAT#: SC108743

LSM7 (untagged)-Human LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LSM7
• Synonyms: YNL147W
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108743 sequence for NM_016199 edited (data generated by NextGen Sequencing)
  ATGGCGGATAAGGAGAAGAAGAAAAAGGAGAGCATCTTGGACTTGTCCAAGTACATCGAC
  AAGACGATCCGGGTAAAGTTCCAGGGAGGCCGCGAAGCCAGTGGAATCCTGAAGGGCTTC
  GACCCACTCCTCAACCTTGTGCTGGACGGCACCATTGAGTACATGCGAGACCCTGACGAC
  CAGTACAAGCTCACGGAGGACACCCGGCAGCTGGGCCTCGTGGTGTGCCGGGGCACGTCC
  GTGGTGCTAATCTGCCCGCAGGACGGCATGGAGGCCATCCCCAACCCCTTCATCCAGCAG
  CAGGACGCCTAG
  
  Clone variation with respect to NM_016199.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016199 unedited
  CGGTCAATTTGATACACTATTAGGCGGCCGGATTCGCCGAGCGAAGACCCACGGCGCGAC
  AAGATGGCGGATAAGGAGAAGAAGAAAAAGGAGAGCATCTTGGACTTGTCCAAGTACATC
  GACAAGACGATCCGGGTAAAGTTCCAGGGAGGCCGCGAAGCCAGTGGAATCCTGAAGGGC
  TTCGACCCACTCCTCAACCTTGTGCTGGACGGCACCATTGAGTACATGCGAGACCCTGAC
  GACCAGTACAAGCTCACGGAGGACACCCGGCAGCTGGGCCTCGTGGTGTGCCGGGGCACG
  TCCGTGGTGCTAATCTGCCCGCAGGACGGCATGGAGGCCATCCCCAACCCCTTCATCCAG
  CAGCAGGACGCCTAGCCTGGCCGGGGGCGCGGGGGGTGCAGGGCAGGCCCGAGCAGCTCG
  GTTTCCCGCGGACTTGGCTGCTGCTCCCACCGCAGTACCGCCTCCTGGAACGGAAGCATT
  TTCCTTTTTGTATAGGTTGAATTTTTGTTTTCTTAATAAAATTGCAAACCTCNNAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAACCCCCTCCTCTTTAAATGGGGCCCGGGGCCTTAT
  TTGTTTCTGTAACAAACCCGGGGGGGATTCCTTGGAACCCCTCCCAATGCCCTTTCTGGG
  CCTGGAAATTGGCACTTCAGTGCCCCCCACCTTTGCCTTAAAAAATAAAGTGGACCTTTT
  GGTCGGACAGGGGGCCTTTTAAAAATAAGGGGGTGAGGGGGGTGGGTTTTTGCACCAGGG
  CCCAATTTGGAAAAAAACCCTGTGGGGCCCCGGGGGGTTTTTTGGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016199
• Insert Size: 540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016199.1, NP_057283.1
• RefSeq Size: 489 bp
• RefSeq ORF: 312 bp
• Locus ID: 51690
• UniProt ID: Q9UK45
• Cytogenetics: 19p13.3
• Domains: Sm
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Spliceosome
• Gene Summary: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Apr 2004]