NOC2L (NM_015658) Human Untagged Clone

CAT#: SC108633

NOC2L (untagged)-Human nucleolar complex associated 2 homolog (S. cerevisiae) (NOC2L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOC2L
• Synonyms: NET7; NET15; NIR; PPP1R112
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015658, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGCTGCGGGGAGCCGCAAGAGGCGCCTGGCGGAGCTGACGGTGGACGAGTTCCTAGCTTCGGGCT
  TTGACTCCGAGTCCGAATCCGAGTCCGAAAATTCTCCACAAGCGGAGACACGGGAAGCACGCGAGGCTGC
  CCGGAGTCCGGATAAGCCGGGCGGGAGCCCCTCGGCCAGCCGGCGTAAAGGCCGTGCCTCTGAGCACAAA
  GACCAGCTCTCTCGGCTGAAGGACAGAGACCCCGAGTTCTACAAGTTCCTGCAGGAGAATGACCAGAGCC
  TGCTAAACTTCAGCGACTCGGACAGCTCTGAGGAGGAAGAGGGGCCGTTCCACTCCCTGCCAGATGTGCT
  GGAGGAAGCCAGTGAGGAGGAGGATGGAGCGGAGGAAGGAGAAGATGGGGACAGAGTCCCCAGAGGGCTG
  AAGGGGAAGAAGAATTCTGTTCCTGTGACCGTCGCCATGGTTGAGAGATGGAAGCAGGCAGCAAAGCAAC
  GCCTCACTCCAAAGCTGTTCCATGAAGTGGTACAGGCGTTCCGAGCAGCTGTGGCCACCACCCGAGGGGA
  CCAGGAAAGTGCTGAGGCCAACAAATTCCAGGTCACGGACAGTGCTGCATTCAATGCTCTGGTTACCTTC
  TGCATCAGAGACCTCATTGGCTGTCTCCAGAAGCTGCTGTTTGGAAAGGTGGCAAAGGATAGCAGCAGGA
  TGCTGCAGCCGTCCAGCAGCCCGCTCTGGGGGAAGCTTCGTGTGGACATCAAGGCTTACCTGGGCTCGGC
  CATACAGCTGGTGTCCTGTCTGTCGGAGACGACGGTGTTGGCGGCCGTGCTGCGGCACATCAGCGTGCTG
  GTGCCCTGCTTCCTGACCTTCCCCAAGCAGTGCCGCATGCTGCTCAAGAGAATGGTGGTCGTATGGAGCA
  CTGGGGAAGAGTCTCTGCGGGTGCTGGCTTTCCTGGTCCTCAGCAGAGTCTGCCGGCACAAGAAGGACAC
  TTTCCTTGGCCCCGTCCTCAAGCAAATGTACATCACGTATGTGAGGAACTGCAAGTTCACCTCGCCTGGT
  GCCCTCCCCTTCATCAGTTTCATGCAGTGGACCTTGACGGAGCTGCTGGCCCTGGAGCCGGGTGTGGCCT
  ACCAGCACGCCTTCCTCTACATCCGCCAGCTCGCCATACACCTGCGCAACGCCATGACCACCCGCAAGAA
  GGAAACATACCAGTCTGTGTACAACTGGCAGTATGTGCACTGCCTCTTCCTGTGGTGCCGGGTCCTGAGC
  ACTGCGGGCCCCAGCGAAGCCCTCCAGCCCTTGGTCTACCCCCTTGCCCAAGTCATCATTGGCTGTATCA
  AGCTCATCCCCACTGCCCGCTTCTACCCGCTGCGAATGCACTGCATCCGTGCCCTGACGCTGCTCTCGGG
  GAGCTCGGGGGCCTTCATCCCGGTGCTGCCTTTCATCCTGGAGATGTTCCAGCAGGTCGACTTCAACAGG
  AAGCCAGGGCGCATGAGCTCCAAGCCCATCAACTTCTCCGTGATCCTGAAGCTGTCCAATGTCAACCTGC
  AGGAGAAGGCGTACCGGGACGGCCTGGTGGAGCAGCTGTACGACCTCACCCTGGAGTACCTGCACAGCCA
  GGCACACTGCATCGGCTTCCCGGAGCTGGTGCTGCCTGTGGTCCTGCAGCTGAAGTCGTTCCTCCGGGAG
  TGCAAGGTGGCCAACTACTGCCGGCAGGTGCAGCAGCTGCTTGGGAAGGTTCAGGAGAACTCGGCATACA
  TCTGCAGCCGCCGCCAGAGGGTTTCCTTCGGCGTCTCTGAGCAGCAGGCAGTGGAAGCCTGGGAGAAGCT
  GACCCGGGAAGAGGGGACACCCCTGACCTTGTACTACAGCCACTGGCGCAAGCTGCGTGACCGGGAGATC
  CAGCTGGAGATCAGTGGCAAAGAGCGGCTGGAAGACCTGAACTTCCCTGAGATCAAACGAAGGAAGATGG
  CTGACAGGAAGGATGAGGACAGGAAGCAATTTAAAGACCTCTTTGACCTGAACAGCTCTGAAGAGGACGA
  CACCGAGGGATTCTCGGAGAGAGGGATACTGAGGCCCCTGAGCACTCGGCATGGGGTGGAAGACGATGAA
  GAGGACGAGGAGGAGGGCGAGGAGGACAGCAGCAACTCGGAGGATGGAGACCCAGACGCAGAGGCGGGGC
  TGGCCCCTGGGGAGCTGCAGCAGCTGGCCCAGGGGCCGGAGGACGAGCTGGAGGATCTGCAGCTCTCAGA
  GGACGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015658 unedited
  GCGCGTCGNAATTTTGTAAACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGGCTTC
  GGGTTGGTGTCATGGCAGCTGCGGGGAGCCGCAAGAGGCGCCTGGCGGAGCTGACGGTGG
  ACGAGTTCCTAGCTTCGGGCTTTGACTCCGAGTCCGAATCCGAGTCCGAAAATTCTCCAC
  AAGCGGAGACACGGGAAGCACGCGAGGCTGCCCGGAGTCCGGATAAGCCGGGCGGGAGCC
  CCTCGGCCAGCCGGCGTAAAGGCCGTGCCTCTGAGCACAAAGACCAGCTCTCTCGGCTGA
  AGGACAGAGACCCCGAGTTCTACAAGTTCCTGCAGGAGAATGACCAGAGCCTGCTAAACT
  TCAGCGACTCGGACAGCTCTGAGGAGGAAGAGGGGCCGTTCCACTCCCTGCCAGATGTGC
  TGGAGGAAGCCAGTGAGGAGGAGGATGGAGCGGAGGAAGGANAAGATGGGGGACAGAGTC
  CCCAGAGGGCTGAAGGGGAAGAAGAATTCTGTTCCTGTGACCGTCGCCATGGTTGAGAGA
  TGGAAGCAGGCAGCAAAGCAACGCCTCACTCCAAAGCTGTTCCATGAAGTGGTACAGGCG
  TTCCGAGCAGCTGTGGCCACCACCCGAGGGGACCAGGAAAGTGCTGAGGCCAACAAATTC
  CAGGTCACGGACAGTGCTGCATTCAATGCTCTGGTTACCTTCTGCATCAGAGACCTCATT
  GGCTGTCTCCAGAAGCTGCTGTTTGGAAAGGTGGGCAAAGATAGCAGCAAGATGCTGCAG
  CCGTCCAGCAGCCCGCTCTGGAGGAAGCTTCGTGTGGACATCAAGGGCTACCTGGGCTCG
  GCCTACAGCTGGTGTCCTGTCTGTCGGAACCACGGTGTTTGGCGGGCGTGCTTGCGCACA
  TCAACGTGCTGGTGCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015658 unedited
  NTTTAGCTCTGGACCCGCGGCCGCAATCTAGNGATCGGNTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGCCCCCCACCGTTTTATTTTTTTCG
  GTTTCGGATGCAAAACAAAAATTTTTAAAAAAAAATGGGACTTCAAAGGAAAAAAACAAT
  TTTCCAAAAACTTGGGGGAGGAAAGGCAAACCCTGGGCCAAATGGACAAGGTCTGCAAAC
  GGAGGGCAAAGGGGGGGAAAGGGGCCAGGGGCCTGCAGGCCTCCCCCTGAAACTGGAACT
  GGTCTCGGTCTGCTGACGTAAGGGTCACCTCCCCCGGGGAGCTGACTTCAGCACCCCACA
  GTTGGGGGGTTTAAGAAGCCACACCAGCCCAGCCCAGCCCAGTTTTGGATACGTTGGGTT
  TTTCATGCTGAAAAAAAAAAAATAAAGCCTGTCCCGGGTTTACTGCCTCCCCCAACTGCA
  CAAACCCCACCCTTTAGGCCTGACTGCCAGGGAGGGGGAAACACTGGCCACCAGCCCGGA
  AGCCCCTACAGGCCCCCCAAAGGGGCTGCCTCAGCCGCCCTCTGAAAGCTGAAAATCCTC
  CAGTTTGTCCTCCGGCCCCTGGGCCAGTTGTTGAAGCTCCCCAGGGGCCAGCCCCGCCTT
  TGCGTTTGGGGTCTCCATCCCAAGACCATTCACCCTCCGAGTTGGTGGGGGCCTCCTCGC
  CCTCCTCCTGGGCCTCTTAATGGGGTCCCACCCCATGCCCGAGGCTCAGGGGCCTCAATA
  TCCTTTCCTCGAAATCCCTGGGGGGCGGCCTCTCAAAACTGGTCAGGAAAAAAAGGCTTT
  AAATGGTTCCTGGCCCAACCCTTCTGGAACCATTTTCTTTGGTTGACTAAAGGAAATAAG
  GTTTTCACCCCTTCTTTCCCGGATCCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015658
• Insert Size: 2740 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015658.1, NP_056473.1
• RefSeq Size: 2762 bp
• RefSeq ORF: 2250 bp
• Locus ID: 26155
• UniProt ID: Q9Y3T9
• Cytogenetics: 1p36.33
• Domains: UPF0120
• Protein Families: Stem cell - Pluripotency
• Gene Summary: Histone modification by histone acetyltransferases (HAT) and histone deacetylases (HDAC) can control major aspects of transcriptional regulation. NOC2L represents a novel HDAC-independent inhibitor of histone acetyltransferase (INHAT) (Hublitz et al., 2005 [PubMed 16322561]).[supplied by OMIM, Mar 2008]