PMF1 (NM_007221) Human Untagged Clone

CAT#: SC108493

PMF1 (untagged)-Human polyamine-modulated factor 1 (PMF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_007221 edited
  ATGGTGGACACTTTTCTTCAGAAGCTGGTCGCCGCCGGCAGCTACCAGAGATTCACTGAC
  TGCTATAAGTGCTTCTACCAGTTGCAGCCTGCGATGACACAGCGAATCTATGACAAGTTT
  ATAGCTCAGTTGCAGACATCTATCCGGGAGGAAATCTCTGACATCAAAGAGGAGGGGAAC
  CTAGAAGCTGTCTTGAATGCCTTGGATAAAATTGTGGAAGAAGGCAAAGTCCGCAAAGAG
  CCAGCCTGGCGCCCCAGCGGGATCCCAGAGAAGGATCTGCACAGTGTTATAGCACCCTAC
  TTCCTGCAGCAACGGGACACCCTGCGGCGCCATGTGCAGAAACAGGAGGCCGAGAACCAG
  CAGCTGGCAGATGCCGTCCTGGCAGGGCGGAGGCAGGTGGAGGAGCTGCAGCTACAGGTC
  CAGGCCCAGCAGCAGGCCTGGCAGGCTCTACACAGAGAACAGAGGGAGCTGGTTGCTGTG
  CTGAGGGAGCCTGAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007221 unedited
  GGGGTCAGTTTTTGTATACGACTCCTATAGGCGGCCGCGAAATTCGCACGAGGGGGCCGA
  AGCAAGTAGCGCCAATCTAGGCAGCGGCTGTGAGGAAAAAAGCATGAGGGGTCGTCTTCG
  GAATCTGTGCCACCCGGCACTACCATTTCGAGGGTGAAGCTCCTCGACACCATGGTGGAC
  ACTTTTCTTCAGAAGCTGGTCGCCGCCGGCAGCTACCAGAGATTCACTGACTGCTATAAG
  TGCTTCTACCAGTTGCAGCCTGCGATGACACAGCGAATCTATGACAAGTTTATAGCTCAG
  TTGCAGACATCTATCCGGGAGGAAATCTCTGACATCAAAGAGGAGGGGAACCTAGAAGCT
  GTCTTGAATGCCTTGGATAAAATTGTGGAAGAAGGCAAAGTCCGCAAAGAGCCAGCCTGG
  CGCCCCAGCGGGATCCCAGAGAAGGATCTGCACAGTGTTATAGCACCCTACTTCCTGCAG
  CAACGGGACACCCTGCGGCGCCATGTGCAGAAACAGGAGGCCGAGAACCAGCAGCTGGCA
  GATGCCGTCCTGGCAGGGCGGAGGCAGGTGGAGGAGCTGCAGCTACAGGTCCAGGCCCAG
  CAGCAGGCCTGGCAGGCTCTACACAGAGAACAGAGGGAGCTGGTTGCTGTGCTGAGGGAG
  CCTGAGTGAGGAGACCGCCAGCCCCAGAAGCAGAGGGCAGTCAAGGTCAAGAGCCTGTGG
  TCCAGCATGCCTGGCCTGNGCGGGCTACCTCTGAGAACGGCTGAAATGGTGCCCAGTCCA
  TCAGCAGTGATGGAATTTGCTGGAGGACTANGCCAGAGCAAGCCTCACTGCCACTGTGCC
  TTTGNGGCACCTTNGGNGNTGNACATACACCCNCTTTAGATTCCCTCTGTTCTTCTACCT
  GGATAATNCTGGGCATGTNCTCTCTCTCTAGGTCAGTTCAGCTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007221 unedited
  TTTGGACGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTAACAGGGGAGGGAAGGAAGTTTATTTACGGATGGG
  GAAAAAACATGGTAAAGGCCCCTGGGCTGGCCAACAAGGGGGCAATCAGGGGAAATTGAA
  AACAAATTTTCCAACCCAGGGCCAGGGAAAAGGGCTGGGGAACCAACAGGAGGGGGGCGG
  AGGGGCAAAACTTACCTGAACCTAAAAAAAAAAAAACATGGCCAAAAATTATCCAGGTAA
  AAAAAACAGAGGAATTTAAAGGGGGGGTTTGTCCAACCCCAAGGGTGCCCCAAAGGCACA
  GGGGCAGGGAGGCTTGCTTTGGCCTAATCCTCCAACAAATTCCATCACTGGTGATGGACT
  GGGCACCATTTCAGCCGTTCTAAAAGGTACCCCCCCCAGGCCAGGCATGCTGGACCACAG
  GCTTTTGACCTTGACTGCCCTTTGCTTTTGGGGCTGGGGGTCTCCTCACTTAGGCTCCCT
  TAGCACAGAAACCAACTCCCTTTGTTTTTTGTGTAAAACCTGCCAGGCCTGCTGCTGGGC
  CTGGACCTGTANCTGAAACTCCTCCACCTGCCTCCGCCCTGCCAAGACGGTATTTGCCAA
  CTGCTGGTTCTCGGCCTCCTGTTTCTGCACATGGCGCCCCAGGGTGTCCCGTTGCTGCAG
  AAAATAGGGTGCTATAACACTGTGCAAATCCTTCTTTGGGATCCCCTGGGGCGCCCAGCT
  GGTTCTTTGGGGACTTTGCCTTTCTTCACAATTTTTTCAAGGCATTACAAACAGCTCTAA
  GTCTCCCTCCTTTTGATGCAAAAAATCCTTCGCGAAGATGCCGCACCGGACTATAACTTG
  CATAAATCCTGGCACCCCGGCTTACTGGTAAAACCCTTTAACACCCGGATCTTGGTACTG
  CCGGGGAACACCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007221
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007221.1, NP_009152.1
• RefSeq Size: 1057 bp
• RefSeq ORF: 498 bp
• Locus ID: 11243
• UniProt ID: Q6P1K2
• Cytogenetics: 1q22
• Protein Families: Stem cell - Pluripotency
• Gene Summary: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate exon but includes another in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.