ATP5MC3 (NM_001689) Human Untagged Clone

CAT#: SC108460

ATP5G3 (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9) (ATP5G3), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5MC3
• Synonyms: ATP5G3; P3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108460 sequence for NM_001689 edited (data generated by NextGen Sequencing)
  ATGTTCGCCTGCGCCAAGCTCGCCTGCACCCCCTCTCTGATCCGAGCTGGATCCAGAGTT
  GCATACAGACCAATTTCTGCATCAGTGTTATCTCGACCAGAGGCTAGTAGGACTGGAGAG
  GGCTCTACGGTATTTAATGGGGCCCAGAATGGTGTGTCTCAGCTAATCCAAAGGGAGTTT
  CAGACCAGTGCAATCAGCAGAGACATTGATACTGCTGCCAAATTTATTGGTGCAGGTGCT
  GCAACAGTAGGAGTGGCTGGTTCTGGTGCTGGTATTGGAACAGTCTTTGGCAGCCTTATC
  ATTGGTTATGCCAGAAACCCTTCGCTGAAGCAGCAGCTGTTCTCATATGCTATCCTGGGA
  TTTGCCTTGTCTGAAGCTATGGGTCTCTTTTGTTTGATGGTTGCTTTCTTGATTTTGTTT
  GCCATGTAA
  
  Clone variation with respect to NM_001689.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001689 unedited
  TATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCTGGGAAGAGAGGAAGCGG
  GAGAGGAGCCCACGTCGCCTGTCACCCAATATCTCCAGCCGCGCAGTCCCGAAGAGTGTA
  AGATGTTCGCCTGCGCCAAGCTCGCCTGCACCCCCTCTCTGATCCGAGCTGGATCCAGAG
  TTGCATACAGACCAATTTCTGCATCAGTGTTATCTCGACCAGAGGCTAGTAGGACTGGAG
  AGGGCTCTACGGTATTTAATGGGGCCCAGAATGGTGTGTCTCAGCTAATCCAAAGGGAGT
  TTCAGACCAGTGCAATCAGCAGAGACATTGATACTGCTGCCAAATTTATTGGTGCAGGTG
  CTGCAACAGTAGGAGTGGCTGGTTCTGGTGCTGGTATTGGAACAGTCTTTGGCAGCCTTA
  TCATTGGTTATGCCAGAAACCCTTCGCTGAAGCAGCAGCTGTTCTCATATGCTATCCTGG
  GATTTGCCTTGTCTGAAGCTATGGGTCTCTTTTGTTTGATGGTTGCTTTCTTGATTTTGT
  TTGCCATGTAACAAATTACTGCTTGACATGTTGGCATTCATATTAATTACGGATGTAATT
  CTGTGTATCTTACTGTGACTCCGAAACTGTAGTATTGGTGTCATGGGAATGTACGTTATT
  TCCAAAGTCATTTCATTAAAGATGAAAACTTTAAAAAAAAAAAAAAAAAACTCGACTCTA
  GATGGCGGGCCGCGGTCATAGCTTGTTTCTGAACAGATCCCGGTGGCATCCCTGTGACCC
  CTNCCCAGTGCTCTCCTGGCCCTGGAGTTGCCACTCATGCCCACCAGCCTTGTCTATAAA
  TTAGTGCATCTTTTGGCTGACTAGGGTCCTCATAAATATGTGGGGGGGGGGGGGGTGGNT
  TANAAAAGGGAAATTTGGAAAAAACCTGAGGCCCCGGGGGCTATTGGACCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001689 unedited
  CCAGGCCCGGTAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGA
  AACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAAGTT
  TTCATCTTTAATGAAATGACTTTGGAAATAACGTACATTCCCATGACACCAATACTACAG
  TTTTCGGAGTCACAGTAAGATACACAGAATTACATCCGTAATTAATATGAATGCCAACAT
  GTCAAGCAGTAATTTGTTACATGGCAAACAAAATCAAGAAAGCAACCATCAAACAAAAGA
  GACCCATAGCTTCAGACAAGGCAAATCCCAGGATAGCATATGAGAACAGCTGCTGCTTCA
  GCGAAGGGTTTCTGGCATAACCAATGATAAGGCTGCCAAAGACTGTTCCAATACCAGCAC
  CAGAACCAGCCACTCCTACTGTTGCAGCACCTGCACCAATAAATTTGGCAGCAGTATCAA
  TGTCTCTGCTGATTGCACTGGTCTGAAACTCCCTTTGGATTAGCTGAGACACACCATTCT
  GGGCCCCATTAAATACCGTAGAGCCCTCTCCAGTCCTACTAGCCTCTGGTCGAGATAACA
  CTGATGCAGAAATTGGTCTGTATGCAACTCTGGATCCAGCTCGGATCAGAGAGGGGGTGC
  AGGCGAGCTTGGCGCAGGCGAACATCTTACACTCTTCGGNACTGCGCGGCTGGAGATATT
  GNGTGACAGGCGACGTGGGCTCCTCTCCCGCTTCCTCTCTTTCCAGNCCTNNGTGCGAAT
  CGCGGCCGNCCTATAGNGAGTCGTATTACAAAATTCTGACGGNTCACTAAACGAGCTCTG
  CTATATAGACCTCCCACGTACACGCTACCGCCATTTGCGTACGGGGGCGGGGTTATACGA
  CTTNTGGAAGTCCGTGATTTTGTGCCAACAACCCCATGCGCAGGGGGGGAGACTGAATTC
  CGGAGTCACGCTCCCGCCCTTGGGTCTGCAACGCTCCATGAAACGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001689
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001689.2, NP_001680.1
• RefSeq Size: 997 bp
• RefSeq ORF: 429 bp
• Locus ID: 518
• UniProt ID: P48201
• Cytogenetics: 2q31.1
• Domains: ATP-synt_C
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding different proteins have been identified. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (2) has an additional intron sequence in its 3' end, compared to variant 4. This difference results in a protein (isoform A) with a longer C-terminus, compared to isoform B. Variants 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.