DHFRL1 (DHFR2) (NM_176815) Human Untagged Clone

CAT#: SC108348

DHFRL1 (untagged)-Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHFRL1
• Synonyms: DHFRL1; DHFRP4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108348 sequence for NM_176815 edited (data generated by NextGen Sequencing)
  ATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACATGGGCATCGGCAAGAAC
  GGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATTTCCAGAGAATGACCACA
  ACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTAGGAAGACCTGGTTCTCC
  ATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAGTTCTCAGCAGAGAACTC
  AAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGGATGATGCCTTAAAACTT
  ACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGATAGTTGGTGGCAGTTCT
  GTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTATTTGTGACAAGGATCATG
  CAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGGAGAAATATAAACTTCTG
  CCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAACACATCAAGTACAAATTT
  GAAGTATGTGAGAAGGATGATTAA
  
  Clone variation with respect to NM_176815.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176815 unedited
  NGGGGGTTCAGCAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACA
  CATCTGCGCGCCTGTGCGGCCCCTGCTTCTGCGGATGCTGAGGCACGTAAAAAAATTTGA
  AGAAGGGAATTTCGCGGCATTCTTGGCCTGGCTTCCTGGCGTAGCCAGCAAGTTCGGAGG
  TGTTAACCGCTGCTGTCATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACA
  TGGGCATCGGCAAGAACGGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATT
  TCCAGAGAATGACCACAACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTA
  GGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAG
  TTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGG
  ATGATGCCTTAAAACTTACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGA
  TAGTTGGTGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTAT
  TTGTGACAAGGATCATGCAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGG
  AGAAATATAAACTTCTGCCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAAC
  ACATCAAGTACAAATTTGAAGTATGTGAGAAGGATGATTAATATGAAANGGTGTTTCTGG
  TTTAAGNTGTTCCCCCTCCCTCTGAGAAAAGTATGCATTTTTACATTAGAAAAAGGACTT
  TTGTTGACTTCAGATCTATGGNATAATTATTTCTAAGCACGTGTTTTTATTCCTCACTAA
  TCTGGCCTTNTCCAGATCCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_176815 unedited
  NNGGGTCCTGNCATGNACCGCGGCACGCAATCTAAGATCGAGTTTTTTTTTTTTTTTTTT
  ACTTTTGCTTTATCTTCAATGCACTTCTTTCCAAATGCCCCAAATCTGGCAATAACCTAT
  TATTTTTAAGAGAAGGGATCTCACTATGGTGGCCAGGCTGCTCTGAACTCTTGGGCTCAA
  GCCATCCTTTTGCTTAGCCTCCTGAGTAGCTGGGACTACAAGCATGGGCCATCCCACCCA
  GCTAATCTATTAGTATTTTTACTGTTTCTTTTTAAAGCTAGCTCCATAGCTGGAATATTG
  CCTGTGTTGGATACTATTTGCTGGATCCACGTCATTCTCTGGTTTGTGTACGCCTTCATT
  TTGCTGGAGTATTTTCTCCGATAGCTTCCCAAGAAAGGGTACATGTAACCCCTGAGTCTT
  TGCTTGTCTAAAAATGTATTATTTTACCTTCACCCTTGATTCTTTGCCTGAATATAGATT
  TATCAGTTGAAAATAACTTTATAGAATTCTGAAGGCATGGTTCCGTTGTTTTCAGATTCT
  TTTTTGAGACAGGGTCTCTTCTGTCACCCAGGCAGGAGTGCAGTGGCAAAATCACGACTC
  ACTGCAGCCTTGAACTCATGGGCTCAATTGAATCCTCCATCTCAGCCCCGTGAGTAGACG
  AGACTACAGGTGCGTGCCACAATGCCCAGCCAATTTTTGTATCTTCTGTAGAGACAGGGC
  TCCACCATGTTGCCCAAGCTGGTCTCAAGTGATCCACCCACCTCATCCCCCCAAATGCTG
  GGACCCACAGCGTGAGCCACCCCTGCTGGCCAGTTTTAAATTCCCTCCCTAACTCTTTCA
  TTTCTCAAAGGGCTCTCAACCTCGTGGGGCCCAATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_176815
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176815.2, NP_789785.1
• RefSeq Size: 3772 bp
• RefSeq ORF: 564 bp
• Locus ID: 200895
• UniProt ID: Q86XF0
• Cytogenetics: 3q11.2
• Protein Families: Druggable Genome
• Gene Summary: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.