CCDC69 (NM_015621) Human Untagged Clone

CAT#: SC108313

CCDC69 (untagged)-Human coiled-coil domain containing 69 (CCDC69)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCDC69
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015621, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCTGCAGACACAGCAGGCTGAGCAGCTGCAAACCCCCGAAAAAGAAGCGCCAAGAACCAGAACCAG
  AACAGCCACCCAGACCAGAGCCCCATGAATTAGGTCCCCTCAATGGGGACACAGCTATAACTGTCCAGCT
  CTGTGCATCAGAGGAGGCTGAGCGGCACCAGAAGGATATAACCAGAATTCTCCAGCAACATGAGGAGGAA
  AAGAAGAAATGGGCACAACAGGTGGAGAAGGAAAGGGAGCTAGAGCTTCGAGACAGACTGGATGAGCAGC
  AAAGGGTCCTGGAAGGAAAGAATGAAGAGGCCCTGCAAGTCCTCCGGGCCTCATATGAACAGGAGAAAGA
  AGCGCTTACCCACTCTTTCCGGGAGGCCAGTTCTACCCAGCAGGAGACCATAGACAGACTGACCTCACAG
  CTGGAGGCTTTCCAGGCCAAAATGAAGAGGGTGGAGGAGTCCATTCTGAGCCGAAACTATAAGAAACATA
  TCCAGGATTATGGGAGCCCCAGCCAGTTCTGGGAGCAGGAGCTGGAGAGCTTACACTTTGTCATCGAGAT
  GAAGAATGAGCGTATTCATGAGCTGGACAGGCGGCTGATCCTCATGGAAACAGTGAAAGAGAAAAATCTG
  ATATTGGAGGAAAAAATTACGACCCTGCAACAGGAAAATGAGGACCTCCATGTCCGAAGCCGCAACCAGG
  TGGTCCTGTCAAGGCAGCTGTCAGAAGACCTGCTTCTCACGCGTGAGGCCCTGGAGAAGGAGGTGCAGCT
  GCGGCGACAGCTCCAGCAGGAGAAGGAGGAGCTGTTGTACCGGGTCCTTGGGGCCAATGCCTCGCCTGCC
  TTCCCTCTGGCCCCTGTCACTCCCACTGAGGTCTCTTTCCTCGCCACATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015621 unedited
  GAGGTTCGAATTATGTNATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGGAG
  GCTGAGCGGCACCAGNAGGGAATAACCAGAATTCTCCAGCAACATGAGGAGGAAAAGAAG
  AAATGGGCACAACAGGTGGAGAAGGAAAGGGAGCTAGAGCTTCGAGACAGACTGGATGAG
  CAGCAAAGGGTCCTGGAAGGAAAGAATGAAGAGGCCCTGCAAGTCCTCCGGGCCTCATAT
  GAACAGGAGAAAGAAGCGCTTACCCACTCTTTCCGGGAGGCCAGTTCTACCCAGCAGGAG
  ACCATAGACAGACTGACCTCACAGCTGGAGGCTTTCCAGGCCAAAATGAAGAGGGTGGAG
  GAGTCCATTCTGAGCCGAAACTATAAGAAACATATCCAGGATTATGGGAGCCCCAGCCAG
  TTCTGGGAGCAGGAGCTGGAGAGCTTACACTTTGTCATCGAGATGAAGAATGAGCGTATT
  CATGAGCTGGACAAGCGGCTGATCCTCATGGAAACAGTGAAAGAGAAAAATCTGATATTG
  GAGGAAAAAATTACGACCCTGCAACAGGAAAATGAGGACCTCCATGTCCGAAGCCGCAAC
  CAGGTGGTCCTGTCAAGGCAGCTGTCAGAAGACCTGCTTCTCACGCGTGAGGCCCTGGAG
  AAGGAGGTGCAGCTGCGGCGACAGCTCCAGCAGGAGAAGGAGGAGCTGTTGTACCGGGTC
  CTTGGGGCCAATGCCTCGNCTGCCTTCCCTCTGGCCCCTGTCACTTCCACTGAGGTCTCT
  TTCCTCGCCACATAGGGTGCAGGGCCTGGGCCCACCACGACGCCTGAAGTCACAGCTTCT
  TCCCAAGTTTTCCTGCAGAAGACAGCAGAGCCTCTCAGTTCTTTTCCAGCAAGGAACCAG
  TTGGGGAGCGATAAGGAGACCTGGGGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015621 unedited
  ATCTTGNCCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTAAGGTGCCGCA
  TGTTCTTTTTAGTTTCCATACATCGTCTGTCCCAGAGTGAGGAGAAGTTGTTCTCCTTCC
  CACATCCACCACAGGCTGCGTGAGGGAAGCCTGGCTCCCCACAACTTGCTCCTTCTCCAG
  CCCTGCCCCTCTCAATTAAAACAATGCTTTCTTTTTTCTTTTCTTTTTTTTGAGACGGAG
  TCTTGCTCTGTCACCCGGGCTGGAGTGCAGTGGCGCGATCTTGGCTCACTGCAAGCTCCG
  CCTCCTGGGTTCACACCATTCTCCAGCCTCAGCCTCCCAAGCTGCTGGGACTACAGGCGC
  CCACCACCACGCCAAGCTAATTTTTTGTATTTTTTTAGTAGAGACAGGGTTTCACTGTGT
  TAGCCAGGATGGTCTCAATCTCCCAACCTTGTGATCCACCCACCTCGGCCTCCCAAAGTG
  CTGGGATTACAGGCGTGAGCCACTGTGCCTGGACTAAAACAATGCTTTCTAAAGCGCATT
  CTGCAGCCTGATGTGCCTGTGAGGTGACAGTTGTGGGAGGGACAAAACCTCTGCTCAAAG
  AGGTTTGGGAGAGGCCGGATACTTACCTCCCTTCTTGTAAGTTTGACACACACATTGGCC
  ATAATAAAGGTCGCGGAAAAACATTCAGGCCACTGATCTGGTTAGGCGCCCCCCAAAAAC
  TTGGGCACCAATCACCAGGACCTAGTGGACATTATGTGAATAAACTAGTGTTATCCCAAA
  CGCGCCTCGCAAAAACCTCTCTTGAAAAAGTCCCCCGGTAATCCCCTCCAGGGCCTTCAG
  TCAAGGCTCCCGGGGGCACCCGCCCCGCACTGGCTCGGAAGATCAACGCCAGACCCCGCG
  GAGAGCTGGCCCCGTGAGCAAATTGGCGTGGGGCAATGGGCACACACCCCCCCGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015621
• Insert Size: 3220 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015621.1, NP_056436.1
• RefSeq Size: 3416 bp
• RefSeq ORF: 609 bp
• Locus ID: 26112
• UniProt ID: A6NI79
• Cytogenetics: 5q33.1
• Gene Summary: May act as a scaffold to regulate the recruitment and assembly of spindle midzone components. Required for the localization of AURKB and PLK1 to the spindle midzone.[UniProtKB/Swiss-Prot Function]