SKIP (INPP5K) (NM_130766) Human Untagged Clone

CAT#: SC108235

INPP5K (untagged)-Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SKIP
• Synonyms: MDCCAID; PPS; SKIP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108235 sequence for NM_130766 edited (data generated by NextGen Sequencing)
  ATGGATGTGCTTTCCCCTCTGAGCTTCATCAAGGTCTCCCATGTCCGTATGCAGGGGATC
  CTCTTACTGGTCTTTGCCAAGTATCAGCATTTGCCCTATATCCAGATTCTGTCTACTAAA
  TCCACCCCCACTGGCCTGTTTGGGTACTGGGGGAACAAAGGTGGAGTCAACATCTGCCTG
  AAGCTTTATGGCTACTATGTCAGCATCATCAACTGCCACCTGCCTCCCCACATTTCCAAC
  AATTACCAGCGGCTGGAGCACTTTGACCGGATCCTGGAGATGCAGAATTGTGAGGGGCGA
  GACATCCCAAACATCCTGGACCACGACCTCATTATCTGGTTTGGAGACATGAACTTTCGG
  ATCGAGGACTTTGGGTTGCACTTTGTTCGGGAATCCATTAAAAATCGGTGCTACGGTGGC
  CTGTGGGAGAAGGACCAGCTCAGCATTGCCAAGAAACATGACCCGCTGCTCCGGGAGTTC
  CAGGAGGGCCGCCTACTCTTCCCGCCCACCTACAAGTTTGATAGGAACTCCAACGACTAT
  GACACCAGTGAGAAAAAACGCAAGCCTGCATGGACCGATCGCATCCTGTGGAGGCTGAAG
  CGGCAGCCCTGTGCTGGCCCCGACACTCCCATACCGCCGGCGTCACACTTCTCCTTGTCT
  CTGAGGGGCTACAGCAGCCACATGACGTACGGCATCAGCGACCACAAGCCTGTCTCCGGC
  ACGTTCGACTTGGAGCTGAAGCCATTGGTGTCTGCTCCGCTGATCGTCCTGATGCCCGAG
  GACCTGTGGACCGTGGAAAATGACATGATGGTCAGCTACTCTTCAACCTCGGACTTCCCC
  AGCAGCCCGTGGGACTGGATTGGACTGTACAAGGTGGGGCTGCGGGACGTTAATGACTAC
  GTGTCCTATGCCTGGGTCGGGGACAGCAAGGTCTCCTGCAGCGACAACCTGAACCAGGTT
  TACATCGACATCAGCAATATCCCTACCACTGAAGATGAGTTTCTCCTCTGTTACTACAGC
  AACAGTCTGCGTTCTGTGGTGGGGATAAGCAGACCCTTCCAGATCCCGCCTGGCTCCTTG
  AGGGAGGACCCACTGGGTGAAGCACAGCCACAGATCTGA
  
  Clone variation with respect to NM_130766.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130766 unedited
  GTTCGAATTTTGGTATACCGACTCCTATAGGGCGGGCCGCGATTCGGCACCGGGGTCGGA
  GTCCGGAAGAAAAACAGTCCGCGACAGCTAGGCGCGTGAGACCGGCCGCCCGCAGGGCTG
  CTCTGGCCGGGACCCGCTGGCCGGGAGACGCGAACCTGCCGGACCACCGCGCGGGGACGA
  CGGCGGCCATGAGCTCGCGGAAGCTGAGCGGGCCGAAAGGCAGGAGGCTCAGCATACACG
  TCGTGACTTGGAACGTGGCTTCGGCAGCGCCCCCTCTAGATCTCAGTGACCTGCTTCAGC
  TGAACAACCGGAACCTCAATCTTGACATATATGTTATTGGTTTGCAGGAATTGAACTCTG
  GGATCATAAGCCTCCTTTCCGATGCTGCCTTTAATGACTCGTGGAGCAGTTTCCTCATGG
  ATGTGCTTTCCCCTCTGAGCTTCATCAAGGTCTCCCATGTCCGTATGCAGGGGATCCTCT
  TACTGGTCTTTGCCAAGTATCAGCATTTGCCCTATATCCAGATTCTGTCTACTAAATCCA
  CCCCCACTGGCCTGTTTGGGTACTGGGGGAACAAAGGTGGAGTCAACATCTGCCTGAAGC
  TTTATGGCTACTATGTCAGCATCATCAACTGCCACCTGCCTCCCCACATTTCCAACAATT
  ACCAGCGGCTGGAGCACTTTGACCGGATCCTGGAGATGCAGAATTGTGAGGGGCGAGACA
  TCCCANACATCCTGGACCACGACCTCATTATCTGGTTTGGAGACATGAACTTTCGGATCG
  AGGATTTTGGGGTTGCACTTTGTTCCGGGATCCATTAAAAAATCTGGCTACCGTGGCCTG
  GGGGGAGAAGGACCAGCTCAGCATTGCCAAGAAACATGACCCGCTGCTCCGGGAGGTCCA
  GGAAGGGCCGGCTACCTTTCCCGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130766 unedited
  CCGCAATCTANAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTAAAACCCACGCTAATAAT
  TACCCCTGCACAATGTAACAGTCACAAAACAGCCAGCCATGGCCTGCTGGAAGCCAGGGG
  ACAATTCTTCATACACAGGCTTGCGGGGGAGAGTATGCAACACAGAGCAACTGGAGTAAC
  CCTCAGTCCCAGGACACAGGATGGCAGGACAAGCACTGAGCAGGCTCCCAGAGGTGTCTT
  CTCCTGGGAAAAGGGCTGCCGAGTGACCATGGCGGAGGCAAGTGCTGGATGTGGGGACAG
  GCCATGTCCCCAAAGCCCCTAAAGGGTGTTCTCTGAAGAACCCCAGGGCCAGATCCTCCA
  AAATGTCTTGAGCTGGTTCTCCCTTCTGCCTGCCCCCACTCTGAGGTCCCGGGGGGTCCA
  GGGGGGGCCCGTCGTCAATGGCTCCTGACAAGGGTGGCACAGACGCACCTCACCTGCCAA
  GGACCACCCTGGAAAAGTCTGTGGCCACAGCTCCTTGACCACTTGTCACCCCTTCCTCAA
  ATGGCAGGGTGGAGGCATGGGACTCAAGCTACCAACGGAAGCGAACCTGGATCCTTCCCA
  GTTCTCTCTGTAAGGATCTGGAACTTCATGCAGTCACACTCCATGCCGTGAGTAGGGAGG
  AAAGTTGTGCTGGCCAAAGACCCTATAAACCTGCTCATACCATGGCGTAAAACCCTGGCT
  AGAGGGGCCCCTCCATTCTGCCCACTGAGTATAACTGCTTTTGCTGAAACCAAAATCCAA
  ACCCTTTGCTCCTTCTCACGGGGACACCCTTGACAGGGCCGACTCCCGGAATCACCATTG
  GTTGCCCATGTCTATGTAGCTGAAAAAGGGGAGCATTTCCCTCCCAAAAGCCTGGGCCCA
  CACGGGCCAAAAGAAGTCGGCCGTGCAAAGGGCCTGTCCTGCAGGACTCTTGGCAAATCA
  AAGGGGGTGGATTCCAACAAACTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130766
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130766.1, NP_570122.1
• RefSeq Size: 3002 bp
• RefSeq ORF: 1119 bp
• Locus ID: 51763
• UniProt ID: Q9BT40
• Cytogenetics: 17p13.3
• Domains: IPPc, Exo_endo_phos
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Inositol phosphate metabolism, Insulin signaling pathway, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (2) has an additional segment in its 5' end, as compared to variant 1. The resulting isoform, isoform 2, has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform, isoform 2.