APBB2 (NM_173075) Human Untagged Clone

CAT#: SC108222

APBB2 (untagged)-Human amyloid beta (A4) precursor protein-binding, family B, member 2 (APBB2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APBB2
• Synonyms: FE65L; FE65L1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108222 sequence for NM_173075 edited (data generated by NextGen Sequencing)
  ATGTCAGAAGTACTTCCAGCTGACTCAGGTGTTGACACCTTGGCAGTGTTTATGGCCAGC
  AGCGGAACTACAGACGTCACAAATCGGAACAGCCCAGCCACACCACCAAACACCCTTAAC
  CTCCGATCCTCCCACAATGAACTGTTGAACGCTGAAATAAAACACACAGAAACCAAGAAC
  AGCACACCTCCCAAATGCAGGAAAAAATATGCACTAACTAACATCCAGGCGGCCATGGGC
  CTCTCGGATCCAGCTGCACAGCCCCTGCTGGGAAATGGCTCTGCCAACATCAAGCTGGTG
  AAAAATGGGGAGAACCAGCTCCGTAAGGCTGCAGAGCAAGGGCAGCAGGACCCCAACAAA
  AACCTGAGCCCCACTGCAGTCATCAACATAACTTCTGAGAAGTTAGAGGGTAAAGAGCCC
  CACCCACAGGATTCCTCGAGCTGTGAGATTTTACCCTCCCAGCCCAGGAGAACTAAGAGC
  TTCCTAAATTACTATGCAGATCTGGAAACCTCAGCCAGAGAACTAGAGCAGAACCAAGGC
  AATCACCATGGGACTGCGGAAGAGAAATCCCAGCCAGTCCAGGGCCAGGCCTCCACCATC
  ATTGGGAATGGTGATTTGCTGCTGCAGAAACCAAACAGACCCCAGTCCAGCCCTGAAGAC
  GGCCAAGTAGCCACAGTGTCATCCAGCCCAGAAACCAAGAAGGATCATCCGAAAACAGGG
  GCCAAAACCGACTGTGCACTGCACCGGATCCAGAACCTGGCACCGAGCGATGAGGAGTCC
  AGCTGGACAACGTTGTCCCAAGACAGTGCCTCACCCAGCTCCCCGGATGAAACAGATATA
  TGGAGTGATCACTCATTTCAGACTGATCCAGATTTGCCGCCTGGCTGGAAAAGAGTCAGT
  GACATTGCCGGGACCTATTATTGGCACATCCCAACAGGAACGACTCAGTGGGAACGGCCC
  GTCTCCATCCCAGCAGATCTCCAGGGTTCTAGGAAAGGGTCACTTAGTTCTGTAACGCCA
  TCTCCCACCCCAGAGAACGAGGATTTGCATGCAGCCACTGTTAACCCGGACCCCAGTTTA
  AAAGAGTTTGAAGGAGCAACCCTACGCTATGCATCTTTGAAACTCAGAAATGCCCCACAC
  CCTGATGATGATGATTCTTGTAGTATCAACAGTGACCCAGAAGCCAAGTGTTTTGCTGTG
  CGTTCTCTGGGATGGGTAGAGATGGCAGAAGAGGACCTCGCCCCCGGTAAAAGTAGTGTT
  GCGGTCAACAACTGCATCAGGCAACTTTCCTACTGCAAAAATGACATCCGAGACACAGTC
  GGGATTTGGGGAGAGGGGAAAGACATGTACCTGATCCTGGAGAATGACATGCTCAGCCTG
  GTGGACCCCATGGACCGCAGCGTGCTGCACTCGCAGCCCATCGTCAGCATCCGCGTGTGG
  GGCGTGGGCCGCGACAATGGCCGGGATTTTGCTTATGTAGCAAGAGATAAAGATACAAGA
  ATTTTGAAATGTCATGTATTTCGATGTGACACACCAGCAAAAGCCATTGCCACAAGTCTC
  CACGAGATCTGCTCCAAGATTATGGCTGAACGGAAGAATGCCAAAGCGCTGGCCTGCAGC
  TCCTTACAGGAAAGGGCCAATGTGAACCTCGATGTCCCTTTGCAAGATTTTCCAACACCA
  AAGACTGAGCTGGTCCAGAAGTTCCACGTGCAGTACTTGGGCATGTTACCTGTAGACAAA
  CCAGTCGGAATGGATATTTTGAACAGTGCCATAGAAAATCTTATGACCTCATCCAACAAG
  GAGGACTGGCTGTCAGTGAACATGAACGTGGCTGATGCCACTGTGACTGTCATCAGTGAA
  AAGAATGAAGAGGAAGTCTTAGTGGAATGTCGTGTGCGATTCCTGTCCTTCATGGGTGTT
  GGGAAGGACGTCCACACATTTGCCTTCATCATGGACACGGGGAACCAGCGCTTTGAGTGC
  CACGTTTTCTGGTGCGAGCCTAATGCTGGTAACGTGTCTGAGGCGGTGCAGGCCGCCTGC
  ATGTTACGATATCAGAAGTGCTTGGTAGCCAGGCCGCCTTCTCAGAAAGTTCGACCACCT
  CCACCGCCAGCAGATTCAGTAACCAGAAGAGTCACAACCAATGTAAAACGAGGGGTCTTA
  TCCCTCATTGACACTTTGAAACAGAAACGCCCTGTCACCGAAATGCCATAG
  
  Clone variation with respect to NM_173075.4
  536 g=>a;612 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173075 unedited
  GCGAATTCGGCACGAGGCTGGTCTCAAGTAGGAAGCTAGTGCGCTGTAACCGCATCTGAT
  CTGGGCGCTCCGGGAAGGGCGAGACTGGAGCAGAGCCGCTGGGCGCCGGAGCCGAGGCGA
  GCGCCGCGCGCACCACTGGTTGGAGTTGCTGTGGGTGAGCTGCTGTGGTCTGTAGCCAAG
  CATGCTGTGGTCGGATCTGCCCAGCCGTGGAACAGAAACATTTGCTGGATGGAAAATCCA
  TAAAAGAAAGCTCCTGTGAAAAGCTGAGGCTGACAATAATTTAAGCAAAATCAGATCGAT
  CTCTTTGGGCTGCCTGACCTCCTTGGGTGCTTGCTATTAATTAACAGACTTTGTGGGGAA
  AAAAAGGAGCTTGCCTTCTGAGCTTTGTACCAAAGACCTGGGAAAACTAACCATCTCAGT
  CTTTCCTGAGGACTTGGGAACTGCCGAGGCCTCTGCCAATGTGTTGACTGTCGCTATGGG
  CTCACTGTTGTCCAGGCAGCTCATATTTCAAATTATAACCTATTTCCTGCACCATTGCTG
  ACGCCTGGGTGATCCATGTCAGAAGTACTTCCAGCTGACTTCAGGTGTTGACACCTTGGC
  AGTGTTTATGGCCAGCAGCGGAACTACAGACGTCACANATCGGAACAGCCCAGCCACACC
  ACCAAACACCCTTAACCTCCGATCCTCCACAATGAACTGNNNTGACGCTGAATANACACA
  CAGAAACCAGACAGCACACCTNCCAATGCANGAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173075
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173075.2, NP_775098.2
• RefSeq Size: 4564 bp
• RefSeq ORF: 2211 bp
• Locus ID: 323
• UniProt ID: Q92870
• Cytogenetics: 4p14-p13
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene interacts with the cytoplasmic domains of amyloid beta (A4) precursor protein and amyloid beta (A4) precursor-like protein 2. This protein contains two phosphotyrosine binding (PTB) domains, which are thought to function in signal transduction. Polymorphisms in this gene have been associated with Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (3) uses two alternate in-frame splice sites and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.