PHPT1 (NM_014172) Human Untagged Clone

CAT#: SC108149

PHPT1 (untagged)-Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHPT1
• Synonyms: CGI-202; HEL-S-132P; HSPC141; PHP; PHP14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108149 sequence for NM_014172 edited (data generated by NextGen Sequencing)
  ATGGCGGTGGCGGACCTCGCTCTCATTCCTGATGTGGACATCGACTCCGACGGCGTCTTC
  AAGTATGTGCTGATCCGAGTCCACTCGGCTCCCCGCTCCGGGGCTCCGGCTGCAGAGAGC
  AAGGAGATCGTGCGCGGCTACAAGTGGGCTGAGTACCATGCGGACATCTACGACAAAGTG
  TCGGGCGACATGCAGAAGCAAGGCTGCGACTGTGAGTGTCTGGGCGGCGGGCGCATCTCC
  CACCAGAGTCAGGACAAGAAGATTCACGTGTACGGCTATTCCATGGCCTATGGTCTTGCC
  CAGCACGCCATTTCAACTGAGAAAATCAAAGCCAAGTACCCCGACTACGAGGTCACCTGG
  GCTAACGACGGCTACTGA
  
  Clone variation with respect to NM_014172.4
  296 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014172 unedited
  GCACGAGGCCGGGAGGAGGAACATGGCGGTGGCGGACCTCGCTCTCATTCCTGATGTGGA
  CATCGACTCCGACGGCGTCTTCAAGTATGTGCTGATCCGAGTCCACTCGGCTCCCCGCTC
  CGGGGCTCCGGCTGCAGAGAGCAAGGAGATCGTGCGCGGCTACAAGTGGGCTGAGTACCA
  TGCGGACATCTACGACAAAGTGTCGGGCGACATGCAGAAGCAAGGCTGCGACTGTGAGTG
  TCTGGGCGGCGGGCGCATCTCCCACCAGAGTCAGGACAAGAAGATTCACGTGTACGGCTA
  TTCCATGGCCTATGGTCTTGCCCAGCACGCCATTTCAACTGAGAAAATCAAAGCCAAGTA
  CCCCGACTACGAGGTCACCTGGGCTAACGACGGCTACTGAGCACTCCCAGCCCGGGGCCT
  GCTGCCTCCAGCAGCCACTTCAGAGCCCCCGCCTTTGCCTGCACTCCTCTTGCAGGGCTG
  GCCCTGCCTGCTCCTGCGGCAGCCTCTGGTGACGTGCTGTCCACCAGGCCTTGGAGACAG
  GCTAGCCTGGCCACAGAATTAAACGTGTTGCCACACCTGCCGGCTTCTGAAAAAAAAAAA
  AAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014172
• Insert Size: 660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014172.2, NP_054891.2
• RefSeq Size: 903 bp
• RefSeq ORF: 378 bp
• Locus ID: 29085
• UniProt ID: Q9NRX4
• Cytogenetics: 9q34.3
• Domains: Ocnus
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
• Gene Summary: This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 3, which is shorter than isoform 4.