SH3KBP1 (NM_031892) Human Untagged Clone

CAT#: SC108004

SH3KBP1 (untagged)-Human SH3-domain kinase binding protein 1 (SH3KBP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SH3KBP1
• Synonyms: AGMX2; CD2BP3; CIN85; GIG10; HSB-1; HSB1; IMD61; MIG18
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108004 sequence for NM_031892 edited (data generated by NextGen Sequencing)
  ATGGTGGAGGCCATAGTGGAGTTTGACTACCAGGCCCAGCACGATGATGAGCTGACGATC
  AGCGTGGGTGAAATCATCACCAACATCAGGAAGGAGGATGGAGGCTGGTGGGAGGGACAG
  ATCAACGGCAGGAGAGGTTTGTTCCCTGACAACTTTGTAAGAGAAATAAAGAAAGAGATG
  AAGAAAGACCCTCTCACCAACAAAGCTCCAGAAAAGCCCCTGCACGAAGTGCCCAGTGGA
  AACTCTTTGCTGTCTTCTGAAACGATTTTAAGAACCAATAAGAGAGGCGAGCGACGGAGG
  CGCCGGTGCCAGGTGGCATTCAGCTACCTGCCCCAGAATGACGATGAACTTGAGCTGAAA
  GTTGGCGACATCATAGAGGTGGTAGGAGAGGTAGAGGAAGGATGGTGGGAAGGTGTTCTC
  AACGGGAAGACTGGAATGTTTCCTTCCAACTTCATCAAGGAGCTGTCAGGGGAGTCGGAT
  GAGCTTGGCATTTCCCAGGATGAGCAGCTATCCAAGTCAAGTTTAAGGGAAACCACAGGC
  TCCGAGAGTGATGGGGGTGACTCAAGCAGCACCAAGTCTGAAGGTGCCAACGGGACAGTG
  GCAACTGCAGCAATCCAGCCCAAGAAAGTTAAGGGAGTGGGCTTTGGAGACATTTTCAAA
  GACAAGCCAATCAAACTAAGACCAAGGTCAATTGAAGTAGAAAATGACTTTCTGCCGGTA
  GAAAAGACTATTGGGAAGAAGTTACCTGCAACTACAGCAACTCCAGACTCATCAAAAACA
  GAAATGGACAGCAGGACAAAGAGCAAGGATTACTGCAAAGTAATATTTCCATATGAGGCA
  CAGAATGATGATGAATTGACAATCAAAGAAGGAGATATAGTCACTCTCATCAATAAGGAC
  TGCATCGACGTAGGCTGGTGGGAAGGAGAGCTGAACGGCAGACGAGGCGTGTTCCCCGAT
  AACTTCGTGAAGTTACTTCCACCGGACTTTGAAAAGGAAGGGAATAGACCCAAGAAGCCA
  CCGCCTCCATCCGCTCCTGTCATCAAACAAGGGGCAGGCACCACTGAGAGAAAACATGAA
  ATTAAAAAGATACCTCCTGAAAGACCAGAAATGCTTCCAAACAGAACAGAAGAAAAAGAA
  AGACCAGAGAGAGAGCCAAAACTGGATTTACAGAAGCCCTCCGTTCCTGCCATACCGCCA
  AAAAAGCCTCGGCCACCTAAGACCAATTCTCTCAGCAGACCTGGCGCACTGCCCCCGAGA
  AGGCCGGAGAGACCGGTGGGTCCGCTGACACACACCAGGGGTGACAGTCCAAAGATTGAC
  TTGGCCGGCAGTTCGCTATCTGGCATCCTGGACAAAGATCTCTCGGACCGCAGCAATGAC
  ATTGACTTAGAAGGTTTTGACTCCGTGGTATCATCTACTGAGAAACTCAGTCATCCGACC
  ACAAGCAGACCAAAAGCTACAGGGAGGCGGCCTCCGTCCCAGTCCCTCACATCTTCATCC
  CTTTCAAGCCCTGATATCTTCGACTCCCCAAGTCCCGAAGAGGATAAGGAGGAACACATT
  TCACTTGCGCACAGAGGAGTGGACGCGTCAAAGAAAACTTCCAAGACTGTTACCATATCC
  CAAGTGTCTGACAACAAAGCATCCCTGCCGCCCAAGCCGGGGACCATGGCAGCAGGTGGC
  GGTGGGCCAGCCCCTCTGTCCTCAGCGGCGCCCTCCCCCCTGTCATCCTCTTTGGGAACA
  GCTGGACACAGAGCCAACTCCCCGTCTCTGTTCGGCACGGAAGGAAAACCAAAGATGGAG
  CCTGCGGCCAGCAGCCAGGCGGCCGTGGAGGAGCTAAGGACACAGGTCCGCGAGCTGAGG
  AGCATCATCGAGACCATGAAGGACCAGCAGAAACGAGAGATTAAACAGTTATTGTCTGAG
  TTGGATGAAGAGAAGAAAATCCGGCTTCGGTTGCAGATGGAAGTGAACGACATAAAGAAA
  GCTCTACAATCAAAATGA
  
  Clone variation with respect to NM_031892.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031892 unedited
  GCACGAGGGGAAAGAACTAGTCTGGNNTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATTT
  CCACTCGCGGGGAGCAGGAAACCCGGCGCAGCCGGGCGCATTGGGCCGCGATGCAACAGC
  AGCAGCAGGAGTCGCCCCGGGGCAGCAGCGGCAGCAGCAGCAGAGGCAGCAGCGGGCGGC
  GCTGAGCCGCCGCCGCCGCCACTGAGGAAGAAGCCGGCCCAGCCGCCGCCGCGTCCGGAC
  CCTCGCGCCTGGATCCCAGCGCCCCGATCCCGGGGCCCCAACCCCCACGCCCGCCTCCGC
  CAACTTTCACGCTGCCTCGGCGGCCCGGCCCGGCTCGACGCCAATGGTGGAGGCCATAGT
  GGAGTTTGACTACCAGGCCCAGCACGATGATGAGCTGACGATCAGCGTGGGTGAAATCAT
  CACCAACATCAGGAATGAGGATGGAGGCTGGTGGGAGGGACAGATCAACGGCAGGAGAGG
  TTTGTTCCCTGACAACTTTGTANGAGAAATAAAGAAAGAGATGAAGAAAGACCCTCTCAC
  CAACAAAGCTCCAGAAAAGCCCCTGCACGAAGTGCCCAGTGGAAACTCTTTGCTGTCTTC
  TGAAACGATTTTAAGAACCAATAAGAGAGGCGAGCGACGGAGGCGCCGGTGCCAGGTGGC
  ATTCAGCTACCTGCCCCAGAATGACGATGAACTTGAGCTGAAAGTTGGCGACATCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031892 unedited
  NTCGTCTTGGACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTAATTTT
  TTTGAATATGGTAGCTGCATCACAAAAGCTCCAATCAAGAGGGTGGTAAAAAGAAGGCAC
  AGATCATTGCAAATACCAGATTTTCTCTTTCTCTTTTTGTAAATATACCGTCATATATAA
  GCTAAAATTTCTCTTAGGGTGGTGGGGTTTGCTGGCAGGGAAAGGGACAGGTTGAACCTA
  CAATTGCATATGTAATAGAATCCTAATGGGGGGACAAAGAGCGAGGCAGCTCTTAGCCTG
  AAGCAAAAGAAAAGGACACTCGCTTCTGACTACGTGTAGTACAAACACTAGGGAAGTCTG
  TGACAAAACACGATTAAAAAATCATAAAATTATAAAGCTTGTAAACGAATAATCTGAAAT
  TACATTTTATTAATGGAAAATATCATCAAAATAGTACCACTATGGACTAAACTGCCTGAG
  TTTTCATTTCGCATTGAGATCTCAGAGTAGAAAAGCCTTTAGCACAATTTTGCTCTGTGT
  AAGTCACAACGAGTGCCAGCCCCCAGACTAAACCCTGNGGAAGATTCTCCTCTTGGATGG
  AATTTGNGTTGCGCTATCAAGACTTTTGTGCTAATCTTTCAAACAGGGACATTTTGAAGC
  AAACCCTTAATCTTTTGGACAAGATTATTTTTGGCTGGGGCAGAAAATTGAGGTCCGGAC
  CTCAGAAGATAAGGTGACATTTAATTGATCAAGTACTCTTTTGGATTGGAGAGCTTCCTT
  TATGGCGTACACTTCCATCTGGCACCCGAACCCGGATTTTTTTCTGTTTACTCCCACTAA
  ACACATAACGGTGGAATTCGCCGACACTGCGGGAGCTTCTCGGGACTCGAGAAGCTCCTC
  AACCCCCGACCTGCGCACTTATCACTCGCCGCCCCACCGGTTCTGACCACAATACCTA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_031892
• Insert Size: 3160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031892.1, NP_114098.1
• RefSeq Size: 3348 bp
• RefSeq ORF: 1998 bp
• Locus ID: 30011
• UniProt ID: Q96B97
• Cytogenetics: Xp22.12
• Domains: SH3
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).