sphingosine 1 phosphate phosphatase 1 (SGPP1) (NM_030791) Human Untagged Clone

CAT#: SC107997

SGPP1 (untagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: sphingosine 1 phosphate phosphatase 1
• Synonyms: SPP-1; SPPase1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107997 sequence for NM_030791 edited (data generated by NextGen Sequencing)
  ATGTCGCTGAGGCAGCGCCTGGCCCAGCTGGTTGGCCGTCTGCAGGACCCGCAGAAAGTG
  GCCCGTTTCCAGCGGCTGTGCGGGGTGGAAGCGCCGCCGCGCCGCTCAGCAGACCGGAGG
  GAGGATGAGAAAGCGGAGGCGCCTCTCGCCGGAGACCCTCGACTGCGAGGGCGGCAGCCA
  GGGGCGCCTGGAGGCCCCCAGCCTCCCGGGAGCGACCGCAATCAGTGCCCGGCCAAGCCG
  GACGGCGGCGGCGCCCCCAACGGCGTGCGGAACGGGCTGGCGGCCGAGCTGGGCCCGGCC
  TCGCCGCGGCGCGCGGGCGCTCTGCGCCGCAACTCGCTGACGGGCGAGGAGGGCCAGCTG
  GCCCGCGTGAGCAACTGGCCGCTCTACTGCCTGTTCTGCTTCGGCACGGAGCTGGGCAAC
  GAACTCTTCTACATCCTGTTCTTCCCCTTCTGGATCTGGAACCTGGACCCTCTGGTGGGC
  CGGAGGCTCGTGGTCATCTGGGTGCTGGTCATGTACCTGGGCCAGTGCACCAAGGACATC
  ATCCGCTGGCCGAGGCCCGCCTCGCCGCCCGTGGTCAAGTTGGAGGTCTTCTACAACTCT
  GAGTACAGCATGCCCTCCACCCATGCCATGTCCGGCACCGCCATCCCCATTTCTATGGTC
  CTCCTCACCTATGGCCGCTGGCAGTACCCTCTTATATATGGACTGATTCTTATTCCCTGC
  TGGTGTTCTCTAGTTTGCCTAAGTAGAATTTACATGGGAATGCACTCTATTCTGGATATT
  ATTGCTGGATTCCTATATACCATTTTAATCTTAGCTGTCTTCTATCCATTTGTGGACCTG
  ATTGACAACTTCAACCAAACTCACAAATATGCTCCATTCATCATCATCGGGCTTCATTTA
  GCTTTGGGGATCTTTTCTTTCACTCTTGACACCTGGAGCACATCCCGAGGAGACACAGCC
  GAGATACTAGGAAGTGGTGCTGGAATTGCATGTGGATCTCATGTTACTTATAACATGGGT
  CTAGTATTAGATCCTTCTCTAGATACATTACCTTTAGCTGGGCCCCCCATTACTGTGACT
  CTGTTTGGAAAAGCCATATTGCGGATCCTCATAGGGATGGTATTTGTACTAATAATCAGA
  GATGTAATGAAAAAGATCACCATTCCTTTAGCCTGCAAAATCTTCAATATACCGTGTGAT
  GATATTCGAAAAGCAAGACAGCACATGGAAGTTGAACTTCCTTATCGGTATATTACCTAT
  GGAATGGTTGGTTTCTCCATCACATTTTTTGTTCCTTACATATTTTTCTTTATTGGTATC
  TCTTGA
  
  Clone variation with respect to NM_030791.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030791 unedited
  NNNGGTTCGCAATTTTGTCATACGACTCCTATAGGCGGCCGCGNAATTCGCACCAGAAGC
  GCTGTGCCGGTTCCGCTGCCGCTGGCCAGGAGCGCGGGGACAGTTCGGGGCTGCGCCGGA
  GGCCGGCCCGCCTTCCCGGGGGTTCCGTTATCATGTCGCTGAGGCAGCGCCTGGCCCAGC
  TGGTTGGCCGTCTGCAGGACCCGCAGAAAGTGGCCCGTTTCCAGCGGCTGTGCGGGGTGG
  AAGCGCCGCCGCGCCGCTCAGCAGACCGGAGGGAGGATGAGAAAGCGGAGGCGCCTCTCG
  CCGGAGACCCTCGACTGCGAGGGCGGCAGCCAGGGGCGCCTGGAGGCCCCCAGCCTCCCG
  GGAGCGACCGCAATCAGTGCCCGGCCAAGCCGGACGGCGGCGGCGCCCCCAACGGCGTGC
  GGAACGGGCTGGCGGCCGAGCTGGGCCCGGCCTCGCCGCGGCGCGCGGGCGCTCTGCGCC
  GCAACTCGCTGACGGGCGAGGAGGGCCAGCTGGCCCGCGTGAGCAACTGGCCGCTCTACT
  GCCTGTTCTGCTTCGGCACGGAGCTGGGCAACGAACTCTTCTACATCCTGTTCTTCCCCT
  TCTGGATCTGGAACCTGGACCCTCTGGTGGGCCGGAGGCTCGTGGTCATCTGGGTGCTGG
  TCATGTACCTGNGCCAGTGCACCAAGGACATCATCCGCTGGCCGAGGCCCGCCTCGCCGC
  CCGTGGTCAAGTTGGAGGTCTTCTACAACTCTGAGTACAGCATGCCCTCCACCCATGCCA
  TGTCCGGCACCGNCATCCCCATTTCTATGGTCCTNCTCACCTATGGCCGCTGGCAGTACC
  CTCTTATATATGGACTGATTCTTATCCCTGCTGGNNNGTCTCTAGTTGCCTAAGTAGAAT
  TACATGGGAATGCACTCTATTCTGGATATATNGCTGGATCCTATATAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030791 unedited
  GAGCACAAATTATGNTACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTT
  TAAACTTCAATTTGGGCTTTTAATTTTAAAATACATTTAGCATAGAACTGTCATAGGACA
  GAAAATAATAAAACACAAATGGAACATTTTCAAATACCTTTTAAGCTAGACATAAAAATC
  AACAGGATAGCATAGGCCCAAATTATGGGAAACGGTCCCTAAAATTCAATTCATTAAACA
  TACATTTTGAAAGTAAAGCTCTTTTCACATTTTCCAACGTACCAATATTTTCCTACATGC
  CTTGGTTTCCTTTTAACTAATAAGTAAAGGGTAAATTTAGTTGCTTTACTTAAAATTACC
  AGCTTCAGTTTTGGTAAAAATTACCATGCCCCTAAATTCTCAATCAGAATTATAAAAATA
  TGAGCTCAAATAGTTATGGTGCCCAAAATAGAAACATCTGAAGTGATAGTTCTGGATAAT
  CATACAGATATTGCACTAAAATCAGTAATTTCATACCTGGTATTTATTTATATGGAAAGT
  TAATCTCTTCTTTATGAAAAACTTTCTACAAATACACTTACTATTAACGTAAGTACAACC
  ACACTTCAAAATATAAAGGTAGACAGAAAAAAGAATAGTTTAATACTATACGCAAATTTT
  CCACTGTGAAAATAATAGAAAGTTGAAAAAATAATAGGATCTAAAATACTGATACTAATT
  TTAGGTTTTATAATTTACCTTAAATAGCCAATAAATGGGTATTAAAAGTTAACGGTGCTA
  AATCCAATCCTTCCAAATTATTTAGAAAATGCTTTGTTTACATATGAAGTATGTGCATTG
  TCCCATCTATAAATTTCACGTTTCCATTCACTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030791
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030791.2, NP_110418.1
• RefSeq Size: 3323 bp
• RefSeq ORF: 1326 bp
• Locus ID: 81537
• UniProt ID: Q9BX95
• Cytogenetics: 14q23.2
• Domains: acidPPc
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Sphingolipid metabolism
• Gene Summary: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009]