CBLB (NM_170662) Human Untagged Clone

CAT#: SC107022

CBLB (untagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CBLB
• Synonyms: Cbl-b; Nbla00127; RNF56
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107022 sequence for NM_170662 edited (data generated by NextGen Sequencing)
  ATGGCAAACTCAATGAATGGCAGAAACCCTGGTGGTCGAGGAGGAAATCCCCGAAAAGGT
  CGAATTTTGGGTATTATTGATGCTATTCAGGATGCAGTTGGACCCCCTAAGCAAGCTGCC
  GCAGATCGCAGGACCGTGGAGAAGACTTGGAAGCTCATGGACAAAGTGGTAAGACTGTGC
  CAAAATCCCAAACTTCAGTTGAAAAATAGCCCACCATATATACTTGATATTTTGCCTGAT
  ACATATCAGCATTTACGACTTATATTGAGTAAATATGATGACAACCAGAAACTTGCCCAA
  CTCAGTGAGAATGAGTACTTTAAAATCTACATTGATAGCCTTATGAAAAAGTCAAAACGG
  GCAATAAGACTCTTTAAAGAAGGCAAGGAGAGAATGTATGAAGAACAGTCACAGGACAGA
  CGAAATCTCACAAAACTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAAGCAATC
  TTTCCCAATGGTCAATTCCAGGGAGATAACTTTCGTATCACAAAAGCAGATGCTGCTGAA
  TTCTGGAGAAAGTTTTTTGGAGACAAAACTATCGTACCATGGAAAGTATTCAGACAGTGC
  CTTCATGAGGTCCACCAGATTAGCTCTGGCCTGGAAGCAATGGCTCTAAAATCAACAATT
  GATTTAACTTGCAATGATTACATTTCAGTTTTTGAATTTGATATTTTTACCAGGCTGTTT
  CAGCCTTGGGGCTCTATTTTGCGGAATTGGAATTTCTTAGCTGTGACACATCCAGGTTAC
  ATGGCATTTCTCACATATGATGAAGTTAAAGCACGACTACAGAAATATAGCACCAAACCC
  GGAAGCTATATTTTCCGGTTAAGTTGCACTCGATTGGGACAGTGGGCCATTGGCTATGTG
  ACTGGGGATGGGAATATCTTACAGACCATACCTCATAACAAGCCCTTATTTCAAGCCCTG
  ATTGATGGCAGCAGGGAAGGATTTTATCTTTATCCTGATGGGAGGAGTTATAATCCTGAT
  TTAACTGGATTATGTGAACCTACACCTCATGACCATATAAAAGTTACACAGGAACAATAT
  GAATTATATTGTGAAATGGGCTCCACTTTTCAGCTCTGTAAGATTTGTGCAGAGAATGAC
  AAAGATGTCAAGATTGAGCCTTGTGGGCATTTGATGTGCACCTCTTGCCTTACGGCATGG
  CAGGAGTCGGATGGTCAGGGCTGCCCTTTCTGTCGTTGTGAAATAAAAGGAACTGAGCCC
  ATAATCGTGGACCCCTTTGATCCAAGAGATGAAGGCTCCAGGTGTTGCAGCATCATTGAC
  CCCTTTGGCATGCCGATGCTAGACTTGGACGACGATGATGATCGTGAGGAGTCCTTGATG
  ATGAATCGGTTGGCAAACGTCCGAAAGTGCACTGACAGGCAGAACTCACCAGTCACATCA
  CCAGGATCCTCTCCCCTTGCCCAGAGAAGAAAGCCACAGCCTGACCCACTCCAGATCCCA
  CATCTAAGCCTGCCACCCGTGCCTCCTCGCCTGGATCTAATTCAGAAAGGCATAGTTAGA
  TCTCCCTGTGGCAGCCCAACAGGTTCACCAAAGTCTTCTCCTTGCATGGTGAGAAAACAA
  GATAAACCACTCCCAGCACCACCTCCTCCCTTAAGAGATCCTCCTCCACCGCCACCTGAA
  AGACCTCCACCAATCCCACCAGACAATAGACTGAGTAGACACATCCATCATGTGGAAAGC
  GTGCCTTCCAGAGACCCGCCAATGCCTCTTGAAGCATGGTGCCCTCGGGATGTGTTTGGG
  ACTAATCAGCTTGTGGGATGTCGACTCCTAGGGGAGGGCTCTCCAAAACCTGGAATCACA
  GCAAGTTCAAATGTCAATGGAAGGCACAGTAGAGTGGGCTCTGACCCAGTGCTTATGCGG
  AAACACAGACGCCATGATTTGCCTTTAGAAGGAGCTAAGGTCTTTTCCAATGGTCACCTT
  GGAAGTGAAGAATATGATGTTCCTCCCCGGCTTTCTCCTCCTCCTCCAGTTACCACCCTC
  CTCCCTAGCATAAAGTGTACTGGTCCGTTAGCAAATTCTCTTTCAGAGAAAACAAGAGAC
  CCAGTAGAGGAAGATGATGATGAATACAAGATTCCTTCATCCCACCCTGTTTCCCTGAAT
  TCACAACCATCTCATTGTCATAATGTAAAACCTCCTGTTCGGTCTTGTGATAATGGTCAC
  TGTATGCTGAATGGAACACATGGTCCATCTTCAGAGAAGAAATCAAACATCCCTGACTTA
  AGCATATATTTAAAGGGAGATGTTTTTGATTCAGCCTCTGATCCCGTGCCATTACCACCT
  GCCAGGCCTCCAACTCGGGACAATCCAAAGCATGGTTCTTCACTCAACAGGACGCCCTCT
  GATTATGATCTTCTCATCCCTCCATTAGGTGAAGATGCTTTTGATGCCCTCCCTCCATCT
  CTCCCACCTCCCCCACCTCCTGCAAGGCATAGTCTCATTGAACATTCAAAACCTCCTGGC
  TCCAGTAGCCGGCCATCCTCAGGACAGGATCTTTTTCTTCTTCCTTCAGATCCCTTTGTT
  GATCTAGCAAGTGGCCAAGTTCCTTTGCCTCCTGCTAGAAGGTTACCAGGTGAAAATGTC
  AAAACTAACAGAACATCACAGGACTATGATCAGCTTCCTTCATGTTCAGATGGTTCACAG
  GCACCAGCCAGACCCCCTAAACCACGACCGCGCAGGACTGCACCAGAAATTCACCACAGA
  AAACCCCATGGGCCTGAGGCGGCATTGGAAAATGTCGATGCAAAAATTGCAAAACTCATG
  GGAGAGGGTTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAGATAGCCCAGAATAATGTC
  GAAGTTGCCCGGAGCATCCTCCGAGAATTTGCCTTCCCTCCTCCAGTATCCCCACGTCTA
  AATCTATAG
  
  Clone variation with respect to NM_170662.3
  1581 g=>a;1863 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170662 unedited
  GGGCGGCCCGCGAATTCGCACGAGGCCTGCTAGTGCTGCTGCGGCGTCCCGCGGCCTCCC
  CGAGTCGGGCGGGAGGGGAGAGCGGGTGTGGATTTGTCTTGACGGTAATTGTTGCGTTTC
  CACGTCTCGGAGGCCTGCGCGCTGGGTTGCTCCTTCTTCGGGAGCGAGCTGTTCTCAGCG
  ATCCCACTCCCAGCCGGGGCTCCCCACACACACTGGGCTGCGTGCGTGTGGAGTGGGACC
  CGCGCACACGCGTGTCTCTGGACAGCTACGGCGCCGAAAGAACTAAAATTCCAGATGGCA
  AACTCAATGAATGGCAGAAACCCTGGTGGTCGAGGAGGAAATCCCCGAAAAGGTCGAATT
  TTGGGTATTATTGATGCTATTCAGGATGCAGTTGGACCCCCTAAGCAAGCTGCCGCAGAT
  CGCAGGACCGTGGAGAAGACTTGGAAGCTCATGGACAAAGTGGTAAGACTGTGCCAAAAT
  CCCAAACTTCAGTTGAAAAATAGCCCACCATATATACTTGATATTTTGCCTGATACATAT
  CAGCATTTACGACTTATATTGAGTAAATATGATGACAACCAGAAACTTGCCCAACTCAGT
  GAGAATGGAGTACTTTAAAATCTACATTGATAGCCTTATGAAAAAGTCAAAACGGGCAAT
  AAGACTCTTNANAGAAGGCAAGGGAGAGAATGTATTGAAGAACAGTCACAGGACAGACGA
  AATCTCACAAACTGTCCCTAATCTTCAGTCACATGCTGGCAGAATCAAGCATCCTCCCCA
  TGGTCATTCCAGGGAGATACTTTCGATACAAAGCAGAGCTGCTGATCTGGGGAAAGAGTT
  TTGGAGACAAACATCGACAATGGAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_170662 unedited
  NNTTTTTTAGCTTGNACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTT
  GCATTAGTTTGATCAGTTGAAAATCAGAACCTTCAAAGCATCTTGTGAATGAGAGAAATG
  GAACTGGACCTGGGCAAGGCATGGGGATGGTAGAGATCCATATGAATAAATGATTTAGCT
  GTTGATGTGGACAGCAAGAGAGTTGGTGGGAAATGTTACAGCAGACCTGACCACGCTACA
  AAGGGTCTGTGAAGAACACAGTACAGGTATCAAAACACCAAAACAAAACTAAACTAAAAA
  CAAGAACAAACTGCAACTTTGCCAAACTGTAAACAAGGTAAAGCATTTCACAGGTTCAAA
  GTTCAAGGGAAGTAAACGTCTTTAAATTATTTTTGTCAGTTCAACCCTGATTTAAAAATA
  TGGCTAGCAAAAACAAGGAAGCCACAGCTGTCGACATCCTGAGCAAGCATCGGTCTCCTT
  TGAGAAGCTGCACTCCCAAGCCTCTTCTCAAGCTGCTACACGAGGAGGAGACACTTCTCT
  CTTGAATTCCATCTCAGTTCTCTTTATTTCCACACTCTTGGAATACATCGATTGCTTTCC
  ATTTTGGTGTCTACAGTTCTGGCTGCTATAGATTTAGACGTGGGGATACTGGAGGAAGGG
  AAGGCAAATTCTCGGAGGATGCTNCGGGGCACTTCGACATTATTCTGGGCTATCTCTAAG
  GCTCTCTTCACCTCTTCAAGGCATACCCTCTCCATGGAGTTTGCAATTTTGCATCGAATT
  TTCCATGCCGCTCAGCCTGGGGGTTTTCTGTGGTGAATTCTGGTGCAGCTTGGCGGCCGG
  TTTAGGGGGGTCTGGTTGGCGCTGTAACATTTGACTGAAGAGTGACTAGCCCGGGAGTTC
  GGAAGTTGCATTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_170662
• Insert Size: 3580 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170662.3, NP_733762.2
• RefSeq Size: 3976 bp
• RefSeq ORF: 2949 bp
• Locus ID: 868
• UniProt ID: Q13191
• Cytogenetics: 3q13.11
• Protein Families: Druggable Genome
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Both variants 2 and 3 encode isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.