KCTD10 (NM_031954) Human Untagged Clone

CAT#: SC106747

KCTD10 (untagged)-Human potassium channel tetramerisation domain containing 10 (KCTD10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCTD10
• Synonyms: BTBD28; hBACURD3; MSTP028; ULRO61
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106747 sequence for NM_031954 edited (data generated by NextGen Sequencing)
  ATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCTGCTACCCGC
  ACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAATGTGGGTGGA
  GCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTGAAGGCCATG
  TTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATTGACCGCTGT
  GGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCTTTACCCGAG
  AGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTCCAAGGCCTG
  GTGGAAGAGTGCCAGGCGGCCCTACAAAACAAAGATACTTATGAGCCTTTCTGCAAGGTC
  CCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAATAAGCCAGCC
  GTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAATTCTGACGAC
  AATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAACGGAAGGGTC
  CTGTTCATAAAGGATGTTATTGGGGATGAAATCTGCTGCTGGTCCTTTTATGGTCAGGGC
  CGGAAGATTGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAGAAACAGACC
  AAGGTGGAGTTTCCCGAAGCCCGGATTTATGAGGAGACCCTGAACATTTTGCTGTATGAG
  GCCCAGGATGGCCGGGGACCTGACAATGCGCTCCTGGAGGCCACAGGCGGGGCGGCGGGG
  CGCTCCCACCACCTGGACGAGGACGAGGAGCGGGAGCGGATCGAGCGCGTGCGGAGGATC
  CACATCAAGCGCCCTGATGACCGGGCCCACCTCCACCAGTGA
  
  Clone variation with respect to NM_031954.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031954 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCTGCGTCCTC
  CGACTTTTCATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCT
  GCTACCCGCACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAAT
  GTGGGTGGAGCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTG
  AAGGCCATGTTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATT
  GACCGCTGTGGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCT
  TTACCCGAGAGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTC
  CAAGGCCTGGTGGAAGAGTGCCAGGCGGCCCTACAAAACAAAGATACTTATGAGCCTTTC
  TGCAAGGTCCCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAAT
  AAGCCAGCCGTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAAT
  TCTGACGACAATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAAC
  GGAAGGGTCCTGTTCATAAAGGATGTTATTGGGGATGAAATCTGCTGCTGGTCCTTTTAT
  GGTCAGGNCCGNAAGAATGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAG
  AAACAGACCAAGGTGGAGTTTTCCGAAGCCCGTATTATGAGGAGACCCCTGACATTTTGC
  TGTATGAAGCCCCAGATGGGCCGGGACCTGACATGCGCTCCTGGAGCCACAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031954 unedited
  TATGGAACGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTGGTTTAAA
  GTTTTATTGTAGACTTTGCTGTTGGATACAAAATGAAGGCATACAACTGTCACAGGCAGG
  GCAGTAAGTACAAAGTCTAAGCTGTAAAAACCGTTTGAAAATATAAACTCGTTTTTGGAA
  TACATGTGTCAAAGGCTGCCCATGTTAATACCTTTGGTATAAAACGGTAACGATTCCCTT
  GACAAACCCATCCATCACCTGACGCACATTCACATCTCCTGGTAACTACTCTACCTAGTC
  TAGTCTCAACCACCCCTGTCAGTCACGACTCACTCCTGTTCCTTTGCAGGTGCAGAGGAG
  CCTGGGAGGTAGGTCACTGAGAACACCCGTCCTACATAGGCGCTGCGCCCAGCCGGGCTC
  CAGCAGGGGCCACCTGTGCCCCACAAGCACACGTCCCCACCCACAGTCACACATATCCCT
  TAAACAAACATGAACACAGACACAAACCAGACCCAAAGTTATCTATCTACCCGCACATCC
  TCAACCTGTTCACTGCTGGCCACTCTACACTGCGCAAAAATCAAAGGCCAAGACAAGGGG
  GCCTGTGCCCATAGCATGGAAGCTCCCTGTGGCCCGTGGCGTTAGGGTTTTCCTTGCTGT
  ATCCCCCCTGGGATGCCAATGGTTTGGGCCACCCGCATTTTTCCTCAACTGTCTTCTCCC
  GTTTCCCCCCTTCTTTCCCCCGCCCCCCCAATGATTTGACCCCCCGTTGCCCACCCCCCC
  CCCTTCCCGCTCCATGCCCAAATTCCTTCCTGTACCGCACCCGCGCCCCCCCCCCCCATT
  TTCACCCCCCTCTCCCCGCTCAATTCCTCTTCCTGATCGGCCACCCCTTCCGCGCCTTGC
  CCCAGCACACAGATCGTGGTGTACCCATTATTATTTTCGTTCATGTTTTTGTTTTCTCCC
  CACTTTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031954
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031954.3, NP_114160.1
• RefSeq Size: 4057 bp
• RefSeq ORF: 942 bp
• Locus ID: 83892
• UniProt ID: Q9H3F6
• Cytogenetics: 12q24.11
• Domains: BTB, K_tetra
• Protein Families: Ion Channels: Other
• Gene Summary: The protein encoded by this gene binds proliferating cell nuclear antigen (PCNA) and may be involved in DNA synthesis and cell proliferation. In addition, the encoded protein may be a tumor suppressor. Several protein-coding and non-protein coding transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1.