ING1 (AF181850) Human Untagged Clone

CAT#: SC106471

(untagged)-Human p33ING1b (ING1) mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ING1
• Synonyms: p24ING1c; p33; p33ING1; p33ING1b; p47; p47ING1a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF181850, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTGAGTCCTGCCAACGGGGAGCAGCTCCACCTGGTGAACTATGTGGAGGACTACCTGGACTCCATCG
  AGTCCCTGCCTTTCGACTTGCAGAGAAATGTCTCGCTGATGCGGGAGATCGACGCGAAATACCAAGAGAT
  CCTGAAGGAGCTAGACGAGTGCTACGAGCGCTTCAGTCGCGAGACAGACGGGGCGCAGAAGCGGCGGATG
  CTGCACTGTGTGCAGCGCGCGCTGATCCGCAGCCAGGAGCTGGGCGACGAGAAGATCCAGATCGTGAGCC
  AGATGGTGGAGCTGGTGGAGAACCGCACGCGGCAGGTGGACAGCCACGTGGAGCTGTTCGAGGCGCAGCA
  GGAGCTGGGCGACACAGCGGGCAACAGCGGCAAGGCTGGCGCGGACAGGCCCAAAGGCGAGGCGGCAGCG
  CAGGCTGACAAGCCCAACAGCAAGCGCTCACGGCGGCAGCGCAACAACGAGAACCGTGAGAACGCGTCCA
  GCAACCACGACCACGACGACGGCGCCTCGGGCACACCCAAGGAGAAGAAGGCCAAGACCTCCAAGAAGAA
  GAAGCGCTCCAAGGCCAAGGCGGAGCGAGAGGCGTCCCCTGCCGACCTCCCCATCGACCCCAACGAACCC
  ACGTACTGTCTGTGCAACCAGGTCTCCTATGGGGAGATGATCGGCTGCGACAACGACGAGTGCCCCATCG
  AGTGGTTCCACTTCTCGTGCGTGGGGCTCAATCATAAACCCAAGGGCAAGTGGTACTGTCCCAAGTGCCG
  GGGGGAGAACGAGAAGACCATGGACAAAGCCCTGGAGAAATCCAAAAAAGAGAGGGCTTACAACAGGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF181850 unedited
  CCCCATTCGGAATTTGTATACGACTCACTATAGGCGGCCGCGNAATCGGCACCAGGNACA
  AGTGCGGCTCGGCGGCCAGCGGAGCGCGCCCCTTCCCGCTGCCCGCTCCGCTCCTCTCTT
  CTACCCAGCCCAGTGGGCGAGTGGGCAGCGGCGGCCGCGGCGCTGGGCCCTCTCCCGCCG
  GTGTGTGCGCGCTCGTACGCGCGGCCCCCGGCGCCAGCCCCGCCGCCTGAGAGGGGGCCT
  GCGCCGCCGGCCGGGGCGTGCGCCCGGGAGCCACCGCCACCGCGGCCCGCGCCCTCAGGC
  GCTGGGGTCCCCGCGGACCCGGAGGCGGCGGACGGGCTCGGCAGATGTAGCCGCCGGGCC
  GAAGCAGGAGCCGGCGGGGGGGCGCCGGGAGAGCGAGGGCTTTGCATTTTGCAGTGCTAT
  TTTTTGAGGGGGGCGGGGGGTGGAGGAAGCGGAAAGCCGCGCCGAGTCGCCGGGGACCTC
  CGGGGTGAACCATGTTGAGTCCTGCCAACGGGGAGCAGCTCCACCTGGTGAACTATGTGG
  AGGACTACCTGGACTCCATCGAGTCCCTGCCTTTCGACTTGCAGAGAAATGTCTCGCTGA
  TGCGGGAGATCGACGCGAAATACCAAGGGATCCTGAAGGAGCTAGACGAGTGCTACGAGC
  GCTTTAGTCGCGAGACAGACGGGGCGCACAAGCGGCGGATGCTGCACTGTGTGCAGCGCG
  CGCTGATCCGCAGCCAGGAGCTGGGCGACGAGAAGATCCAGATCGTGAGCCACATGGTGG
  AACTTGTTGTAGCACCGTACGCGGCAGGTGGACAGCCTACTGGAGCTGTTCGAGGCCCCA
  CAGGAGCTGGGCGACCCACGGGCACCCCGGGCAGGCTGGCCCGGCCGGCCCAAGGCAAGC
  TGCCATTCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AF181850 unedited
  TTATAGGTCTATTATGNACCCGGCCGCATACNATGATCGGTTTTTTTTTTTTTTTTTTGT
  TGGATCAAACCTATTTTGTAGTTTCTAAAAGCTGATCCACAGACACTTTCTTGTTACACG
  TGTACCAATGAAAACAAAAGGCAAACAGAATCACTGCCATCCCTATGAAAGGAATGGTTC
  CTTTTCTAACATTCTTTAAAAATATACATTTTACACTCCTTGCACCTCAACAAAGGCAGC
  AATGTAATAAATACACGGTTTATTTTGTCCTCCTCACACCAGGCGCCTGTCCACAAACTA
  CCTGTTGTAAGCCCTCTCTTTTTTGGATTTCTCCAGGGCTTTGTCCATGGTCTTCTCGTT
  CTCCCCCCGGCACTTGGGACAGTACCACTTGCCCTTGGGTTTATGATTGAGCCCCACGCA
  CGAGAAGTGGAACCACTCGATGGGGCACTCGTCGTTGTCGCAGCCGATCATCTCCCCATA
  GGAGACCTGGTTGCACAGACAGTACGTGGGTTCGTTGGGGTCGATGGGGAGGTCGGCAGG
  GGACGCCTCTCGCTCCGCCTTGGCCTTGGAGCGCTTCTTCTTCTTGGAGGTCTTGGCCTT
  CTTCTCCTTGGGTGTGCCCGAAGCGCCGTCGTCGTGGTCGTGGTTGCTGGACGCGTTCTC
  ACGGTTCTCGTTGTTGCGCTGCCGCCGTGAGCGCTTGCTGTTGGGCTTGTAAACCTGAGC
  TGGCGCCTCGCCTTTGGGCCTGTCCGCGCCAGCCTTGCCGCTGTTGCCCCCTGTGTTGGC
  CCACTCCTGCTGCGCCCTCAACAACTTCAAGTGGCTGGCCCACTGCCGGGTGCCGTTCTT
  CACCAACTCCAACAT
  
• Restriction Sites: NotI-NotI
• ACCN: AF181850
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF181850.1, AAF07921.1
• RefSeq Size: 2897 bp
• RefSeq ORF: 840 bp
• Locus ID: 3621
• Cytogenetics: 13q34
• Domains: PHD
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]