CELF3 (AF329264) Human Untagged Clone

CAT#: SC106400

(untagged)-Human CUG-BP and ETR-3 like factor 3 (CELF3) mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CELF3
• Synonyms: BRUNOL1; CAGH4; ERDA4; ETR-1; TNRC4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF329264, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGGAGCCGGATGCCATCAAGCTGTTTGTGGGGCAGATCCCGAGGCATCTGGAGGAGAAGGACCTGA
  AGCCCATCTTCGAACAGTTTGGTCGGATCTTTGAGCTGACTGTCATCAAGGACAAGTACACCGGGCTGCA
  CAAGGGATGTGCCTTCCTGACATACTGTGCCCGGGATTCAGCCCTGAAGGCCCAGAGCGCCCTGCACGAA
  CAGAAGACGCTTCCAGGGATGAACAGGCCGATCCAGGTCAAGCCAGCCGACAGCGAGAGCCGAGGAGAAG
  ACCGGAAGCTCTTTGTGGGGATGCTAGGGAAGCAGCAGACAGATGAGGACGTCCGGAAGATGTTTGAGCC
  CTTCGGGACCATCGACGAGTGCACTGTGCTCCGGGGGCCAGATGGCACCAGCAAAGGCTGCGCCTTCGTG
  AAGTTCCAGACCCACGCTGAGGCCCAGGCGGCCATCAACACCCTTCACAGCAGCCGGACCCTGCCAGGTG
  CCTCGTCCAGCCTGGTGGTGAAGTTTGCTGACACTGAGAAGGAGCGAGGTCTCCGCCGCATGCAGCAGGT
  GGCCACCCAGTTGGGCATGTTCAGCCCCATCGCCCTCCAGTTTGGAGCCTACAGCGCCTACACCCAGGCC
  CTGATGCAGCAGCAGGCGGCCCTGGTAGCGGCTCACAGTGCCTACCTCAGCCCCATGGCCACCATGGCTG
  CCGTGCAGATGCAGCACATGGCTGCCATCAATGCCAATGGCCTCATCGCCACCCCCATCACCCCATCCTC
  AGGAACCAGCACCCCTCCTGCCATCGCTGCCACGCCTGTCTCTGCCATTCCGGCTGCCCTGGGCGTCAAC
  GGCTACAGCCCGGTGCCCACCCAGCCCACTGGGCAGCCTGCCCCTGATGCTCTGTATCCCAACGGGGTTC
  ACCCCTACCCAGCCCAGAGCCCCGTGGCCCCCGTGGACCCCCTGCAGCAGGCCTACGCGGGGATGCAGCA
  CTACACAGCAGCCTACCCAGCAGCCTACAGCCTGGTTGCACCTGCGTTCCCGCAGCCTCCAGCCCTGGTC
  GCCCAGCAGCCCCCACCACCACCTCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAAAGAGAAGGCC
  CTGATGGCTGCAACATCTTCATCTACCACCTGCCCCAGGAGTTCACTGACTCAGAGATCCTCCAGATGTT
  TGTCCCCTTTGGCCACGTCATCTCAGCCAAAGTCTTTGTTGACCGAGCCACCAATCAAAGCAAATGTTTT
  GGCTTTGTGAGTTTCGACAATCCGGCCAGTGCCCAGGCTGCCATCCAGGCCATGAATGGCTTCCAGATCG
  GCATGAAGCGCCTCAAAGTCCAGCTAAAGCGGCCTAAGGATGCCAACCGGCCCTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF329264 unedited
  ATAGGCCGGCCGCGATTCGGCACGAGGCCGCCTCCAGCTCTCATCTCAACTCCCAGTTCC
  CAGCCCTCTAAGCCCTAGCACCCCCGGCTACCACCTAACCCTCCAGGTCTTGGCCATACT
  CACCCCCATCCTGGGACATTTGCCAGATCTCTGGGAGGGAGATCGTTTGTTTAGGCGATG
  CCCCCTGGTGGCATGACAGCGTCTGCCTAGACCCCTGACCCCTAGCACTCAACTCCCTGG
  GCCTCCTTTGTGTGAAGAGCCGCCCCTCTGCTTAGCAGCGTGGTTGGGGGCCAAAGGGAA
  AGCCAGCCCCAGCTGGGCCCCCGGGCCCCACCACTTATCTCCTTGCTGGGCAGCTCCCCT
  TGGCCTTTGGGCCTCTCCCTGCTCCCCTCGGCCCCTCCTCCTGGGCCGCCTCAATGAAGG
  AGCCGGATGCCATCAAGCTGTTTGTGGGGCAGATCCCGAGGCATCTGGAGGAGAAGGACC
  TGAAGCCCATCTTCGAACAGTTTGGTCGGATCTTTGAGCTGACTGTCATCAAGGACAAGT
  ACACCGGGCTGCCAAGGGATGTGCCTTCCTGACATACTGTGCCCGGGATTCAGCCCTGAA
  GGCCCAGAGCGCCCTGCACGAACAGAAGACGCTTCCAGGGATGAACAGGCCGATCCAGGT
  CAAGCCAGCCGACAGCGAGAGCCGNAGAAGTANGNNTCTGTGCCTTTGGACTCTGTCCCT
  CTTCTNCCCACCCCCAGCCCAGGGGCCTGGAGTGTCNAGGGGTANCCTTTCTTGAGGGAG
  NAAGCCTGATGATTTCTGAGAATTCCCANCTGCGGTGAGAAGGAGTCANTGTGGCCNAAC
  GGNTTAAATCTGGGGGCGGGTACGATGGCAAACTGAGCCCCAGGGGGAAGGCAGATCTTC
  AAGCTGGGTTG
  
• Restriction Sites: NotI-NotI
• ACCN: AF329264
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF329264.1, AAK07474.1
• RefSeq Size: 1389 bp
• RefSeq ORF: 1389 bp
• Locus ID: 11189
• Cytogenetics: 1q21.3
• Domains: RRM
• Protein Families: Transcription Factors
• Gene Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]