NCALD (NM_032041) Human Untagged Clone

CAT#: SC106380

NCALD (untagged)-Human neurocalcin delta (NCALD), transcript variant 8

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCALD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106380 sequence for NM_032041 edited (data generated by NextGen Sequencing)
  ATGGGGAAACAGAACAGCAAGCTGCGCCCGGAGGTCATGCAGGACTTGCTGGAAAGCACA
  GACTTTACAGAGCATGAGATCCAGGAATGGTATAAAGGCTTCTTGAGAGACTGCCCCAGT
  GGACATTTGTCAATGGAAGAGTTTAAGAAAATATATGGGAACTTTTTCCCTTATGGGGAT
  GCTTCCAAATTTGCAGAGCATGTCTTCCGCACCTTCGATGCAAATGGAGATGGGACAATA
  GACTTTAGAGAATTCATCATCGCCTTGAGTGTAACTTCGAGGGGGAAGCTGGAGCAGAAG
  CTGAAATGGGCCTTCAGCATGTACGACCTGGACGGAAATGGCTATATCAGCAAGGCAGAG
  ATGCTAGAGATCGTGCAGGCAATCTATAAGATGGTTTCCTCTGTAATGAAAATGCCTGAA
  GATGAGTCAACCCCAGAGAAAAGAACAGAAAAGATCTTCCGCCAGATGGACACCAATAGA
  GACGGAAAACTCTCCCTGGAAGAGTTCATCCGAGGAGCCAAAAGCGACCCGTCCATTGTG
  CGCCCCCTGCAGTGCGACCCGAGCAGTGCCGGCCAGTTCTGA
  
  Clone variation with respect to NM_032041.2
  545 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032041 unedited
  AGTATATTTGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCC
  GAATTCGGCACGAGGTGAGAATCTGGTGGATGCTGGACCTTGCTGCTGCTGCTACTGCTG
  TTTCCAGGGGCTGCAGAGCATGGACTGTTAAATCTTGCACTTCTTCTGAGTGAGCTGAAT
  TCTTGCCGCCAGGATGGGGAAACAGAACAGCAAGCTGCGCCCGGAGGTCATGCAGGACTT
  GCTGGAAAGCACAGACTTTACAGAGCATGAGATCCAGGAATGGTATAAAGGCTTCTTGAG
  AGACTGCCCCAGTGGACATTTGTCAATGGAAGAGTTTAAGAAAATATATGGGAACTTTTT
  CCCTTATGGGGATGCTTCCAAATTTGCAGAGCATGTCTTCCGCACCTTCGATGCAAATGG
  AGATGGGACAATAGACTTTAGAGAATTCATCATCGCCTTGAGTGTAACTTCGAGGGGGAA
  GCTGGAGCAGAAGCTGAAATGGGCCTTCAGCATGTACGACCTGNACGGAAATGGCTATAT
  CAGCAAGGCAGAGATGCTAGAGATCGTGCAGGCAATCTATAAGATGGTTTCCTCTGTATT
  GAAAATGCCTGAAGATGAGTCAACCCCAGAGAAAAGAACAGAAAAGATCTTCCGCCAGAT
  GGACACCAATAGAGACGGAAAACTCTCCCTGNAAGAGTTCATCCCGAGAGCCAAAAGCGA
  CCCGTCCATTGTGCGCCCCCTGCAGTGCGACCCGAGCAGTGCCCGGCCAGTTCTGACCCC
  TGCGCCCACCCATCCGAATTGTAGAGCTGCTTGGGTTCCCTTTTGATTCTTCTTTTAACA
  ATTTTTTTTTTTTTTTGCCAAACAAAACAATGTGGAAGCCGTCCCCTGTGCGGTCTGATG
  CCCCTTTCTTCGGGAACCCCTAACCTTCTTTTGTCGGGGGAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032041
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032041.1, NP_114430.1
• RefSeq Size: 3300 bp
• RefSeq ORF: 582 bp
• Locus ID: 83988
• UniProt ID: P61601
• Cytogenetics: 8q22.3
• Domains: EFh
• Gene Summary: This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (8) differs in the 5' UTR compared to variant 1.