PCCA (AF385926) Human Untagged Clone

CAT#: SC106305

(untagged)-Human propionyl-CoA carboxylase alpha subunit (PCCA) mRNA, complete cds, nuclear gene for mitochondrial product

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCCA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF385926, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGGTTCTGGGTCGGGACAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGGCCGCCGCAGC
  AGCTGATGCTGAGCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTATTCAAGACAGTGCTTAATGGT
  GTCCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAAAAAACTTTTGATAAAATTCTTGTTGCTAAT
  AGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAGATGGGCATTAAGACAGTTGCCATCCACA
  GTGATGTTGATGCTAGTTCTGTTCATGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCAC
  CAGTAAAAGCTACCTCAACATGGATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACAT
  CCAGGTTATGGATTCCTTTCAGAAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCA
  TTGGACCTGACACACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCTAAGAAAGC
  AGAGGTTAATACAATCCCTGGCTTTGATGGAGTAGTCAAGGATGCAGAAGAAGCTGTCAGAATTGCAAGG
  GAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCAGGTGGTGGTGGGAAAGGCATGCGCATTGCTTGGG
  ATGATGAAGAGACCAGGGATGGTTTTAGATTGTCATCTCAAGAAGCTGCTTCTAGTTTTGGCGATGATAG
  ACTACTAATAGAAAAATTTATTGATAATCCTCGTCATATAGAAATCCAGGTTCTAGGTGATAAACATGGG
  AATGCTTTATGGCTTAATGAAAGAGAGTGCTCAATTCAGAGAAGAAATCAGAAGGTGGTGGAGGAAGCAC
  CAAGCATTTTTTTGGATGCGGAGACTCGAAGAGCGATGGGAGAACAAGCTGTAGCTCTTGCCAGAGCAGT
  AAAATATTCCTCTGCTGGGACCGTGGAGTTCCTTGTGGACTCTAAGAAGAATTTTTATTTCTTGGAAATG
  AATACAAGACTCCAGGTTGAGCATCCTGTCACAGAATGCATTACTGGCCTGGACCTAGTCCAGGAAATGA
  TCCGTGTTGCTAAGGGCTACCCTCTCAGGCACAAACAAGCTGATATTCGCATCAACGGCTGGGCAGTTGA
  ATGTCGGGTTTATGCTGAGGACCCCTACAAGTCTTTTGGTTTACCATCTATTGGGAGATTGTCTCAGTAC
  CAAGAACCGTTACATCTACCTGGTGTCCGAGTGGACAGTGGCATCCAACCAGGAAGTGATATTAGCATTT
  ATTATGATCCTATGATTTCAAAACTAATCACATATGGCTCTGATAGAACTGAGGCACTGAAGAGAATGGC
  AGATGCACTGGATAACTATGTTATTCGAGGTGTTACACATAATATTGCATTACTTCGAGAGGTGATAATC
  AACTCACGCTTTGTAAAAGGAGACATCAGCACTAAATTTCTCTCCGATGTGTATCCTGATGGCTTCAAAG
  GACACATGCTAACCAAGAGTGAGAAGAACCAGTTATTGGCAATAGCATCATCATTGTTTGTGGCATTCCA
  GTTAAGAGCACAACATTTTCAAGAAAATTCAAGAATGCCTGTTATTAAACCAGACATAGCCAACTGGGAG
  CTCTCAGTAAAATTGCATGATAAAGTTCATACCGTAGTAGCATCAAACAATGGGTCAGTGTTCTCGGTGG
  AAGTTGATGGGTCGAAACTAAATGTGACCAGCACGTGGAACCTGGCTTCGCCCTTATTGTCTGTCAGCGT
  TGATGGCACTCAGAGGACTGTCCAGTGTCTTTCTCGAGAAGCAGGTGGAAACATGAGCATTCAGTTTCTT
  GGTACAGTGTACAAGGTGAATATCTTAACCAGACTTGCCGCAGAATTGAACAAATTTATGCTGGAAAAAG
  TGACTGAGGACACAAGCAGTGTTCTGCGTTCCCCGATGCCCGGAGTGGTGGTGGCCGTCTCTGTCAAGCC
  TGGAGACGCGGTAGCAGAAGGTCAAGAAATTTGTGTGATTGAAGCCATGAAAATGCAGAATAGTATGACA
  GCTGGGAAAACTGGCACGGTGAAATCTGTGCACTGTCAAGCTGGAGACACAGTTGGAGAAGGGGATCTGC
  TCGTGGAGCTGGAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF385926 unedited
  GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGC
  ACGAGGGGTCAGCAGAGGGGCGGTCTGCGGGGACAACAATGGCGGGGTTCTGGGTCGGGA
  CAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGGCCGCCGCAGCAGCTGATGCTGA
  GCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTATTCAAGACAGTGCTTAATGGTGT
  CCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAAAAAACTTTTGATAAAATTCTTG
  TTGCTAATAGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAGATGGGCATTA
  AGACAGTTGCCATCCACAGTGATGTTGATGCTAGTTCTGTTCATGTGAAAATGGCGGATG
  AGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTACCTCAACATGGATGCCATCA
  TGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACATCCAGGTTATGGATTCCTTTCAG
  AAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCATTGGACCTGACA
  CACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCTAAGAAAGCAG
  AGGTTAATACAATCCCTGGCTNTGATGGAGTAGTCAAGGATGCAGAAGAAGCTGTCAGAA
  TTGCAAGGGAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCANGTGGTGGTGGGGAAA
  GCATGCGCATTGCTTGGGATGATGAAAAGACAGNGNATGGTTNNTAGATTGTCATCTCAA
  GAAAGCTGCTTCTAGTTTTGGGCGATGATAGACTACTAATAGAANAAATTATTGATNNAT
  CCTCGCATATAGAAATCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AF385926 unedited
  CGCAATCTAGAGTCGAGTNNTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTAAACAAA
  TGCTCAGTTTTATTGATTTAATTTTGACACTAGGGAATCTCACAGCAGAAGTACAAATCA
  TATGTACAAGTATTTATATCAATGAAAATTTCCATTGGTGATTTTTTGGCAAAATATTGG
  TCTTGACTCTGTGGAATAAATGACGACGTAAACGTAGCTGCACAGGGGTGTTCCTGTATA
  ATGCTTGAATCAATTGTGTGTGAAAGCATCATGCAAATGGCTAATTAAATTGGGTGATGA
  CTGAAAGGTTATAAATCCTTCATTCCAGCTCCACGAGCAGATCCCCTTCTCCAACTGTGT
  CTCCAGCTTGACAGTGCACAGATTTCACCGTGCCAGTTTTCCCAGCTGTCATACTATTCT
  GCATTTTCATGGCTTCAATCACACAAATTTCTTGACCTTCTGCTACCGCGTCTCCAGGCT
  TGACAGATACGGCCACCACCACTCCGGGCATCGGGGAACGCAGAACACTGCTTGTGTCCT
  CAGTCACTTTTTCCAGCATAAATTTGTTCAATTCTGCGGCAAGTCTGGTTAAGATATTCA
  CCTTGTACACTGTACCAAGAAACTGAATGCTCATGTTTCCACCTGCTTCTCGAGAAAGAC
  ACTGGACAGTCCTCTGAGTGCCATCAACGCTGACAGACAATAAGGGCGAAAGCCAGTTCC
  ACGTGCTGGTCACATTTAGTTTCGACCCATCAACTTCCACGAGAACACTGACCCATTGTT
  TGATGCTACTACGGTATGAACTTTATTATGCAATTTTACTGAAAGCTCCCATTGGCTATG
  TCTGGTTTAATACAAGCATCCTTGATTTTCTTGAAAAGTTGGGCCTCTTACTGGATGCCC
  CAACATGAGGAGCCTTGCCAAAACTGGTCTCTNACTCTTGGTAACAGGGGGCTTGGACCT
  CCAGATCCCTCGGAAAAATTTAGGCGAGTGCCTTTAAAACGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: AF385926
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF385926.1, AAL66189.1
• RefSeq Size: 2488 bp
• RefSeq ORF: 2187 bp
• Locus ID: 5095
• Cytogenetics: 13q32.3
• Domains: biotin_lipoyl, CPSase_L_D2, CPSase_L_chain, Biotin_carb_C
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]