NMNAT2 (AF288395) Human Untagged Clone

CAT#: SC106206

(untagged)-Human C1orf15 mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NMNAT2
• Synonyms: C1orf15; PNAT2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106206 sequence for AF288395 edited (data generated by NextGen Sequencing)
  ATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGCCTGCGGCAGCTTCAATCCCATC
  ACCAAAGGGCACATTCAGATGTTTGAAAGAGCCAGGGATTATCTGCACAAAACTGGAAGG
  TTTATTGTGATTGGCGGGATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTC
  GTGTCAAGCCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGATC
  AGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGCAGCGTGTTGGAA
  CACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCATCCTCTCCAATGTCAACACACCT
  TCCATGACACCTGTGATCGGACAGCCACAAAACGAGACCCCCCAGCCCATTTACCAGAAC
  AGCAACGTGGCCACCAAGCCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTC
  AGCCGGATCTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATGCC
  AATCTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTGCTGCTGTGTGGT
  AGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTGGAACGAGGCAGATATGGAGGTG
  ATTGTTGGTGACTTTGGGATTGTGGTGGTGCCCCGGGATGCAGCCGACACAGACCGAATC
  ATGAATCACTCCTCAATACTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGAC
  ATCAACCATCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATGGG
  GACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATCCTCAAAAGCCAG
  CTGTACATCAATGCCTCCGGCTAG
  
  Clone variation with respect to AF288395.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF288395 unedited
  NNTTCACTTCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA
  TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG
  CCGCGAATTCGGCACGAGGCACCGCCGGAGCCTCGCCTTCCTTTCTCCCTCTGCAGACAC
  AACGAGACACAAAAAGAGAGGCAACCCCTAGACCACCGCGAAGGACCCATCTGCACCATG
  ACCGAGACCACCAAGACCCACGTTATCTTGCTCGCCTGCGGCAGCTTCAATCCCATCACC
  AAAGGGCACATTCAGATGTTTGAAAGAGCCAGGGATTATCTGCACAAAACTGGAAGGTTT
  ATTGTGATTGGCGGGATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTG
  TCAAGCCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGATCAGG
  GTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGCAGCGTGTTGGAACAC
  CACCGGGACCTCATGAAGAGGGTGACTGGCTGCATCCTCTCCAATGTCAACACACCTTCC
  ATGACACCTGTGATCGGACAGCCACAAAACGAGACCCCCCAGCCCATTTACCAGAACAGC
  AACGTGGCCACCAAGCCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGC
  CGGATCTGCTGTGTCCGCCCGCCCGTGGAGCGTTTCACCTTTGTAGATGAGAATGCCAAT
  CTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTGCTGCTGTGTGGTAGT
  GACCTGCTGGAAGTCTTCTGCATCCCAGGGCTCTGGGAACGAGCAGATAGGGAGGTGATT
  GC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AF288395 unedited
  NGGCCCTACTATTACCGTGCCGCTTTCTAGATCGATTTTTTTTTTTTTTTTTTCTTGTAG
  CATTGTCTTATTTATTAACATAATTGAAACATTTTGCATAAAACTCTTGCCCATGACTAT
  TCTAGCAACAAAATTGTACTCAAAATATTTCACTGTGAAATGGTATTGCAACCTTGAATA
  TCATTTTTTATTAATGAATTGATTTCCATAAAGCAAATCTTACTCTTAAAATGGCAGATT
  ATGTGATCAAAAAGCGATTCAAAAAAGCTTCCCCCTCCTCATGCCAACCCTCAAGACCAT
  GTGGATCCAGCTGAATCCTCACCCTGGGACTAGACTAAGTGAGGAAAACCGTACTCTTCC
  TAACCGACAGCTAATAGACCTCCAACTCCACTACAAAACCCTGACATCATGTGCTGGGCA
  CTATAATGGGCAACGGATGAATGCAACTATTCTCACCTTCTACTGCTTTCTAAGGCCATT
  GTTGACACAATCGCCCTGTTCATCTTCCATCTACTGTTGCCCTCTGCATAGTACCTGGAG
  AATCCGACTTTTCATATTCTACCGTTTATTTTTGCATTACTGTTTGGATGAGTAAAATGT
  AATAACATGCTTCTCTCTCTTAATCCCCTAAAAAATGTGTGTGTGTGTGTGTGTGTGTGT
  GTGTGTGTGTNNNNCCAATTTTGAGGAGAGTAGAAATTGGGCTGGTTAGTACAAATTTTT
  TTCTCCCCCGAAATTCTCTGCCCTTTGCCTGAGTTCAGTTATTATTGAAGCCCACTTTCC
  ACCTAAT
  
• Restriction Sites: NotI-NotI
• ACCN: AF288395
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF288395.1, AAG60615.1
• RefSeq Size: 5691 bp
• RefSeq ORF: 5691 bp
• Locus ID: 23057
• Cytogenetics: 1q25.3
• Domains: CTP_transf_2
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism
• Gene Summary: This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]