AP3S2 (AL110108) Human Untagged Clone

CAT#: SC106189

(untagged)-Human EST from clone 588049, 5' end

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP3S2
• Synonyms: AP3S3; sigma3b
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AL110108, the custom clone sequence may differ by one or more nucleotides
  
  
  CTGAGAGAACCGTGGCTGGCAAAGATGATTCAGGCGATTCTGGTTTTCAACAACCATGGGAAGCCACGGC
  TAGTCCGCTTCTACCAGCGTTTCCCANAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTANT
  CCTCAAGCGGGATGACAACATCTGTAACTTCTTGGAGGGTGGAAGTTTGATTGGTGGCTCTGACTACAAA
  CTGATCTACCGGCACTATGCTACCCTCTACTTTGTATTTTGTGTGGATTCCTCAGAGAGTGAACTTGGAA
  TCTTGGACCTCATCCAGGTTTTTGTGGAAACTCTGGATAAGTGTTTCGAAAATGTGTGTGAATTGGATTT
  GATCTTCCATATGGATAAGGTGCACTACATCCTCCAGGAAGTGGTGATGGGTGGGATGGTGTTGGAAACA
  NACATGAATGAAATCGTGGCTCANATTGAGGCTCAAAACANGCTGGGAGAAATCCCGAAGGGTGGCCTTT
  CAGCAGCCCCTGGCGCGGGCTGTGTCCTGCTGTGAAAAAAACATCAAACCTGGCCAAGAGAATTCCTCCG
  GAACATCAACAATTGGGCGATTTTCAACATCAAAAGTTCCCAAACCT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AL110108 unedited
  TACCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG
  AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
  TTCGGCACGAGGCTTTCTGGGAGTAGCCGGTGCTGAGAGAACCGTGGCTGGCAAAGATGA
  TTCAGGCGATTCTGGTTTTCAACAACCATGGGAAGCCACGGCTAGTCCGCTTCTACCAGC
  GTTTCCCAGAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTAGTCCTCAAGC
  GGGATGACAACATCTGTAACTTCTTGGAGGGTGGAAGTTTGATTGGTGGCTCTGACTACA
  AACTGATCTACCGGCACTATGCTACCCTCTACTTTGTATTTTGTGTGGATTCCTCAGAGA
  GTGAACTTGGAATCTTGGACCTCATCCAGGTTTTTGTGGAAACTCTGGATAAGTGTTTCG
  AAAATGTGTGTGAATTGGATTTGATCTTCCATATGGATAAGGTGCACTACATCCTCCAGG
  AGGTGGTGATGGGTGGGATGGTGTTGGAAACAACATGAATGAATCGTGGCTCAGATTGAN
  GCTCANAACANGCTGNAGAAAATCGAGGNNTGCCTTTCAGCAGNCCCTGCGCGGGCTGTG
  TCTGCTGTGNAAAACATCACCTGCCAGAGATTCCCTCGAAACATCACATTNGCGATCTCA
  ACATCAAATTCCCCAACTGTCCCCAGTTGTCTGAGATCAAGTATGNCCTGAATAGAGTCC
  TTAGACCAGCAAGACAAGCAGGCAGCCCGTCTGGAAACGACCNATTTGGGAGCTAGAGAA
  TCAAGCCTCAGACTGNAAACTTGGTCTGGAAACTGTTTGCATGAATAGAAGGATCCTTTG
  ACCTGCTGGTGCC
  
• Restriction Sites: NotI-NotI
• ACCN: AL110108
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AL110108.1
• RefSeq Size: 607 bp
• RefSeq ORF: 607 bp
• Locus ID: 10239
• Cytogenetics: 15q26.1
• Domains: Clat_adaptor_s
• Protein Pathways: Lysosome
• Gene Summary: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.[UniProtKB/Swiss-Prot Function]