Ribosomal Protein S29 (RPS29) (BC015974) Human Untagged Clone

CAT#: SC106160

RPS29 (untagged)-Homo sapiens, Similar to ribosomal protein L32, clone IMAGE:4105424

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS29
• Synonyms: DBA13; S29
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC015974, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTCACCAGCAGCTGTACTGGAGCCACCCGCGAAAATTCGGCCAGGGTTCTCGCTCTTGTCGTGTCT
  GTTCAAACCGGCACGGTCTGATCCGGAAATATGGCCTCAATATGTGCCGCCAGTGTTTCCGTCAGTACGC
  GAAGGATATCGGTTTCATTAAGAAAGACCTGAGCTGTCTTCCTTGGCACTGCCTATGGAGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC015974 unedited
  CCCCGCCCCGTTGNCNCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGA
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATT
  CGGCACGAGCCAGCTTCTTCCTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACC
  AGCAGCTGTACTGGAGCCACCCGCGAAAATTCGGCCAGGGTTCTCGCTCTTGTCGTGTCT
  GTTCAAACCGGCACGGTCTGATCCGGAAATATGGCCTCAATATGTGCCGCCAGTGTTTCC
  GTCAGTACGCGAAGGATATCGGTTTCATTAAGTTGGACTAAATGCTCTTCCTTCAGAGGA
  TTATCCGGGGCATCTACTCAATGAAAAACCATGATAATTCTTTGTATATAAAATAAACAT
  TTGAAAAAACAAAAAANAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCT
  GTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGANCCCTCCCAGTGCCTCTCCTGGCCN
  TGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTT
  TGTCTGACTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC015974 unedited
  AAAACCTATGTACGCGGCCCCAACCTANGTTCGAGTTAAATCCTTTTTTTTTTGTTTTTT
  CAAATGTTTATTTTATATACAAAGAATTATCATGGTTTTTCATTGAGTAGATGCCCCGGA
  TAATCCTCTGAAGGAAGAGCATTTAGTCCAACTTAATGAAACCGATATCCTTCGCGTACT
  GACGGAAACACTGGCGGCACATATTGAGGCCATATTTCCGGATCAAACCGTGCCGGTTTG
  AACAGACACGACAAGAGCGAGAACCCTGGCCGAATTTTCGCGGGTGGCTCCAGTACAGCT
  GCTGGTGACCCATCTTGCTCTCAGCAGTGCAACGAGGTAAAAGGAAGAAGCTGGCTGGTG
  CCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACG
  AGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGC
  GGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATT
  GACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATT
  GGTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTG
  CCAAGTAGGAAAGTCCCGGTAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACC
  GTCATTGACGTCAATNAGGGGCGGACTTGGCATATGATACACTTGATGTACTGCCAAGTG
  GGCAGTTTACCGTAAATACTCCACCATTGACGTCAATGGAAGTCCCTATTGGCGTTCTAT
  GGGAACATACCTCATTATTGACCTCATGGGCGGGGGTCTTTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: BC015974
• Insert Size: 400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015974.1, AAH15974.2
• RefSeq Size: 749 bp
• RefSeq ORF: 204 bp
• Locus ID: 6235
• Cytogenetics: 14q21.3
• Domains: Ribosomal_L32e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit and a member of the S14P family of ribosomal proteins. The protein, which contains a C2-C2 zinc finger-like domain that can bind to zinc, can enhance the tumor suppressor activity of Ras-related protein 1A (KREV1). It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]