PCGF5 (AL832496) Human Untagged Clone

CAT#: SC105959

(untagged)-Human mRNA, cDNA DKFZp686I1251 (from clone DKFZp686I1251), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCGF5
• Synonyms: RNF159
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105959 sequence for AL832496 edited (data generated by NextGen Sequencing)
  ATGGCTACCCAAAGGAAACACTTGGTGAAAGATTTTAATCCTTACATTACCTGCTATATC
  TGTAAAGGGTATCTGATCAAGCCAACAACAGTGACGGAATGCCTCCATACATTCTGTAAG
  ACTTGTATTGTTCAGCACTTTGAAGATAGCAATGATTGCCCAAGGTGTGGCAACCAAGTT
  CATGAGACAAATCCATTAGAAATGTTGAGGTTGGACAATACATTAGAGGAAATTATATTT
  AAGCTGGTCCCTGGACTACGAGAACAAGAACTTGAGCGTGAATCTGAATTTTGGAAGAAA
  AATAAGCCTCAAGAAAATGGACAAGATGATACTTCAAAAGCTGACAAACCGAAAGTAGAT
  GAAGAAGGTGATGAAAATGAAGATGATAAAGATTATCACAGAAGTGACCCACAAATTGCT
  ATCTGTCTAGATTGTTTACGAAATAATGGGCAATCAGGGGACAATGTAGTAAAGGGTTTA
  ATGAAGAAATTCATTCGATGTTCTACACGTGTAACTGTGGGAACTATTAAAAAATTTCTA
  AGTTTAAAACTAAAACTTCCAAGTTCTTATGAGTTGGATGTGCTGTGCAATGGTGAAATT
  ATGGGGAAGGATCATACTATGGAATTCATCTACATGACAAGATGGCGACTAAGAGGCGAA
  AACTTTCGGTGTCTGAACTGCTCAGCTTCGCAAGTCTGCTCTCAGGATGGCCCTTTGTAT
  CAGTCATACCCTATGGTACTGCAGTATCGACCAAGAATTGATTTCGGTTAG
  
  Clone variation with respect to AL832496.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AL832496 unedited
  TTCACATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCGGCGCG
  GGCTCCCTCACCTACCGCCCCACGCGCGCGCGCTCGCGCACCACGCGCCCCGCGCGGCCC
  GCCCGGATCGTGGCCTCTCGAGAGCAAGACATGGGAAAGCGGAACCACCAAAAGGAGTGA
  TGATCAACGATCTCATGATAAATCTGGATGCTAGTTCTCATGCCTCAGGACATCCTACTG
  GGAACGACACACCAGCTCCTGGGATCAGACTTTCATCTACTTAGGACCCCTCTTTGCCCA
  GACTACTAAAGCCAGTCTTCACTAGCCACGAATGGCTACCCAAAGGAAACACTTGGTGAA
  AGATTTTAATCCTTACATTACCTGCTATATCTGTAAAGGGTATCTGATCAAGCCAACAAC
  AGTGACGGAATGCCTCCATACATTCTGTAAGACTTGTATTGTTCAGCACTTTGAAGATAG
  CAATGATTGCCCAAGGTGTGGCAACCAAGTTCATGAGACAAATCCATTAGAAATGTTGAG
  GTTGGACAATACATTAGAGGAAATTATATTTAAGCTGGTCCCTGGACTACGAGAACAAGA
  ACTTGAGCGTGAATCTGAATTTTGGAAGAAAAATAAGCCTCAAGAAAATGGACAAGATGA
  TACTTCAAAAGCTGACAAACCGAAAGTAGATGAAGAAGGTGATGAAAATGAAGATGATAA
  AGATTATCACAGAAGTGACCCACAAATTGCTATCTGTCTAGATTGTTTACGAAATATGGG
  CAATCAGGNNGACACTGTAGTAAAGGTTTAATGAAGAAATCATTCGATGTTCTACACGTG
  TAACTGTGGGAACTATAAAAAATTNCTAGTTTAAACTAAAACTCCCAAGTCTATGAGTTG
  GAGTGCTGTGCATGGTGAAATATGGGGAGGATCTACTATGGATCACTACTGACAGATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AL832496 unedited
  NNTTCGTTATACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTT
  TTTTTGGAAGTAAAGGTCTTTCAGTTTATTGCTTAAGAAACAACAGATTAAAGAAAACTT
  TTAGTTTTAGTCTCTGATGTTACAGATTCAGATGATTCCTATAGGTTATTTAAAGAATTC
  ATTTTATCATTTTATAAGCACCCTAAATTTATAAAGCTAACCACAAAAGTTGCCTGTACA
  TTTTTTCTATACCTAGTTTCTTGCAAATTCTACAATCTGACTTAAGGGATAATTAACGGG
  GAATACAGTGTATTACTAGACATGAAAGTAGTCCTATCCTTAGAAAGCTGTAGTTGAATA
  TACCAAAATAAGTCAGTTGAAGAAATCTGTGATTCTAGTAGTAATACCATATCACTTAGG
  ACCATCAAAAAAATGTGTACCTTTCTCCAAACGACAACTGATGCCTTTCTTTATGTAAGC
  TTTTCACGTGTTTTGGCACATATTCCATTGCATTTCAATAAAAATGTTAATGCATTATCA
  GTAAATAAAAGAATTCTATATAAAACTATTCTCAGTTTCATAATTGCTTCCTATATATTT
  CCTTGGAAAATAGTGAAATTACATATGACAAGGCAACCAGCCAAATTTAAAGAGATTTTG
  TAGATAAAATTCATTTATGACCATTAAGTTTTGGCTGGGGGGCCAAAACATCAAAGTCTC
  ACTTGTGATTTTTAAAATGCACTCTTAATTCATTTTGAGTTAATTAAAATTCCATTTGCA
  AAACACTATTTTAATCGCTGACCTTAGTGTGACCAATTTATTATAATTTGTNAAAGTTCT
  TAGGGATGTCTGGGGACAGTTTCCACCATAGAAAAAAAATTCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: AL832496
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AL832496.1, CAD91165.1
• RefSeq Size: 3718 bp
• RefSeq ORF: 3718 bp
• Locus ID: 84333
• Cytogenetics: 10q23.32
• Domains: RING
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:26151332). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332). Plays a redundant role with PCGF3 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (By similarity).[UniProtKB/Swiss-Prot Function]