ATG4D (NM_032885) Human Untagged Clone

CAT#: SC102914

ATG4D (untagged)-Human ATG4 autophagy related 4 homolog D (S. cerevisiae) (ATG4D)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATG4D
• Synonyms: APG4-D; APG4D; AUTL4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032885, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACTCAGTGTCGCCGGCCGCCGCGCAGTACCGGAGCAGCAGCCCGGAGGACGCGCGCCGCCGGCCCG
  AGGCCCGCAGGCCGCGGGGTCCCAGAGGCCCAGACCCCAACGGCCTGGGGCCTTCCGGAGCCAGCGGCCC
  CGCTCTTGGCTCTCCCGGGGCTGGCCCGAGTGAGCCGGACGAAGTGGACAAGTTCAAGGCCAAGTTCCTG
  ACAGCCTGGAACAACGTCAAGTACGGTTGGGTGGTTAAAAGCCGGACCAGCTTTAGCAAGATCTCCAGCA
  TCCACCTCTGTGGCCGCCGCTACCGTTTCGAGGGCGAGGGTGACATACAGCGTTTCCAGCGGGACTTTGT
  GTCCCGCCTGTGGCTCACATACCGCCGGGACTTCCCGCCCCTTCCTGGGGGCTGCCTGACCTCGGACTGT
  GGCTGGGGGTGCATGTTACGCAGCGGCCAGATGATGCTGGCACAGGGCCTTCTGCTGCATTTCCTGCCCA
  GAGACTGGACATGGGCCGAGGGCATGGGCCTGGGCCCCCCTGAGCTGTCAGGGTCAGCCTCTCCCAGCCG
  GTACCATGGGCCTGCCCGCTGGATGCCCCCACGCTGGGCCCAGGGTGCCCCTGAGCTGGAGCAGGAACGC
  CGGCACCGGCAGATTGTGTCCTGGTTCGCCGACCACCCCCGGGCCCCCTTTGGCCTACACCGGCTGGTGG
  AGCTTGGGCAGAGCTCAGGCAAGAAGGCAGGTGACTGGTATGGGCCATCGCTAGTGGCACACATCCTCAG
  GAAAGCCGTGGAGAGCTGCTCCGACGTCACCCGCCTGGTGGTGTACGTTTCTCAGGACTGCACAGTGTAC
  AAGGCGGATGTGGCACGCCTGGTGGCCAGGCCAGACCCCACAGCCGAGTGGAAGTCTGTGGTCATCCTGG
  TGCCCGTGCGACTGGGTGGCGAGACTCTCAACCCCGTGTATGTGCCCTGCGTGAAGGAACTCCTGCGTTG
  CGAGCTGTGCCTGGGCATCATGGGTGGGAAACCGCGACACTCACTGTACTTCATTGGCTACCAAGATGAC
  TTCCTGCTGTACCTGGACCCTCACTACTGCCAGCCCACTGTGGATGTCAGCCAGGCCGACTTCCCCCTGG
  AGTCCTTCCACTGCACCTCGCCCCGCAAGATGGCCTTTGCCAAGATGGACCCAAGCTGTACCGTGGGCTT
  CTATGCTGGAGACAGGAAGGAGTTTGAGACACTCTGCTCAGAGCTGACCAGGGTCCTCAGCTCCTCCTCA
  GCCACAGAGCGGTACCCCATGTTCACCCTGGCCGAGGGCCATGCTCAGGACCACAGCCTGGACGACCTCT
  GCTCCCAGCTCGCCCAGCCCACACTCCGGCTCCCTCGCACAGGGCGGCTCCTCAGGGCCAAACGCCCCAG
  CTCTGAGGACTTTGTGTTTTTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032885 unedited
  GCACGAGGCCCGAGTGAGCCTGGACGAATGGACAAGTTCAAGGCCAAGTTCCTGACAGCC
  TGGAACAACGTCAAGTACGGTTGGGTGGTTAAAAGCCGGACCAGCTTTAGCAAGAGCTCC
  AGCATCCACCTCTGTGGCCTGCCGCTACCGTTTCGAGGGCGAGGGTGACATACAGCGTTT
  CCAGCGGGACTTTGTGTCCCGCCTGTGGCTCACATACCGCCGGGACTTCCCGCCCCTTCC
  TGGGGGCTGCCTGACCTCGGACTGTGGCTGGGGGTGCATGTTACGCAGCGGCCAGATGAT
  GCTGGCACAGGGCCTTCTGCTGCATTTCCTGCCCAGAGACTGGACATGGGCCGAGGGCAT
  GGGCCTGGGCCCCCCTGAGCTGTCAGGGTCAGCCTCTCCCAGCCGGTACCATGGGCCTGC
  CCGCTGGATGCCCCCACGCTGGGCCCAGGGTGCCCCTGAGCTGGAGCAGGAACGCCGGCA
  CCGGCAGATTGTGTCCTGGTTCGCCGACCACCCCCGGGCCCCCTTTGGCCTACACCGGCT
  GGTGGAGCTTGGGCAGAGCTCAGGCAAGAAGGAAAGCCGTGGAGAGCTGCTCCGACGTCA
  CCCGCCTGGTGGTGTACGTTTCTCAGGACTGCACAGTGTACAAGGCTGGATGTGGCACGC
  CTGGTGGCCAGGCCAGACCCCCAGCCGAGTGGAAGTCTGTGGTCATCCTGGTGCCCGTGC
  GACTGGGTGGCGAGACTCTCAACCCCGTGTATGTGCCCTGCGTGAAGGACTCCTGCGTGC
  GAGCTGTGCCTGGCATCATGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032885 unedited
  CAGCCACAGTTCCCCCAGGGGGCTTTGGGACAGGGAGGGCCCGGGCGTCCAGTGCAAACT
  ACAGTACGTGAGTCAGGCCCTGAGGGGGGTGCCCGGGGTCCTCCTTCCCTACCCTGATAG
  GAGGGCCCCGCTGGTGGGAGACAGGTGTATGGGCTCTGTGGGCTCTGTCCCTGGCTGAAT
  GGCCCCAGACTGGTTGCAGCTGGGCTTGAGGGGCTGACAACAGGAAGTACCATCATCACT
  GCCAGAGCTCAAGATCCCACACCCGACATGACATAAATAAAAAAATAAATAGTGTTGTAT
  CTTTCCCCTCATCCCCTCCCTTTATAAAAACACAAAGTCCTCAGAGCTGGGGCGTTTGGC
  CCTGAGGAGCCGCCCTGTGCGAGGGAGCCGGAGTGTGGGCTGGGCGAGCTGGGAGCAGAG
  GTCGTCCAGGCTGTGGTCCTGAGCATGGCCCTCGGCCAGGGTGAACATGGNGTACCGCTC
  TGTGGCTGAGGAGGAGCTGANGACCCTGGTCAGCTCTGAGCAGAGTGTCTCANACTCCTT
  CCTGTCTCCAGCATAGAAGCCCACGGTACAGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032885
• Insert Size: 1850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032885.2, NP_116274.2
• RefSeq Size: 1996 bp
• RefSeq ORF: 993 bp
• Locus ID: 84971
• UniProt ID: Q86TL0
• Cytogenetics: 19p13.2
• Domains: Peptidase_C54
• Protein Pathways: Regulation of autophagy
• Gene Summary: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes the longer isoform (1).