SIRT5 (BC035196) Human Untagged Clone

CAT#: SC102256

(untagged)-Homo sapiens, clone IMAGE:5263943

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIRT5
• Synonyms: SIR2L5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC035196, the custom clone sequence may differ by one or more nucleotides
  
  
  AGCGGGATGTGACCCACAGCCCTATTGGCTCAGCATGCGAGAGAGCAGGGATGGTGCTCCCCAAGGAAGG
  ACTGGTGGGCAGGACGGAGCAGCGCATGTCCACTAGGGTTGAAAGTGCTCTGCACGGCACCAGTTGCCTG
  GTACCCATTTCATAAACTGTGGCCACCATGATTGCTTCAGCTCAATAAGTGGTGGTTAGTATTAACAATT
  ACTTTTTTCACTTATTAGTTGATTACTTATTTATTGAGGTGCTTTTGTATAAAAGATTCAAGCTTTCTGA
  AATCGAAGTTAAAATTTTGTAAACATATCCCACGCCATTGAACCTTTGTCAGTCATGTCAATAAATCACG
  CCCTTAGATTTAGGTTGAGCTCCCTCTACCCTATTGGCAACTTCTGCCCACTTGAACACCACTCCAAGGT
  CATCTGATGGCACATTAGTCATGGAGTTATTTATTTTTCCTCCAGATTATAAAGTCCTTGAGGGCATATG
  CTCTTGACTGGCTTAAGAGTACTGCATGCCTCTGTTTCCCATGAAAGCTAAAGCTAGGGTAGGCCCATTT
  GCTAAAATGAAGTATGGCTCAGTGTGGCGGTGCACACCTATAGTCCCAGCTGCTCAGGAGGCAAAGGTGG
  GAGGATCGCTTGAGCCCATGAGTTGGAAGCTGCAGTGAGCTATGATCGTACTGCTGCACTCCAGCCTGGG
  TAACTGGGTGAGACCAAAACATGAAATGAAGTGTGAAAAATAAAGTATCTTATACCCTTTTAGACATGGA
  GAAGGTTGCTCTTGATTATACTAAATTCTGGATTTGCTTCATATGTATTTTTCAGGTAGCTTATTTAAAA
  CTCGATGTACCTCTTGTGGAGTTGTGGCTGAGAATTACAAGAGTCCAATTTGTCCAGCTTTATCAGGAAA
  AGGGTAATTATACCACACTACAGAATAAGTATAGGTTGTTTTCTCCTTTAGGTTGAACATAAATGTGAGG
  AAATTGAACATTCATCAAAGATTCCCATGGATATTTATGAGAAATAAGACTAAATACAAGGCTGGGTGCA
  GTGGCTCATGCCTGTAATCCCAGCACTTTGGGGTTACAAGGTGGGGAGACTGCTTGAGTTCAGGAGTTCA
  AGACCAGCCTGGGCAACACAGTGAGAAACCTGTCCTACAAAAAAACAAAACAAAACAAAAAACCACAAAA
  ATTAGCTGGGTGTGGTGGCACATGCCTGTAGTCCCAGCTACTAGGGAGGCTGAGGTGGGAGGGTTATTTG
  AGCCTGGGAAGTCAAGGCTGCAGTGAGCTGAGATCATGCCACTGCACTACAGCCTGGGTGACAGAGGGAG
  ACCATGTCTCAAAAAACAAACAAACAAACAAAAAAACTAAATATAGCCATGTGATAAAAGAAATATTTTG
  CTCACAACTTACAGTGTTAAGTGAAAACCACAGGGTCCTAAATTTGTACACACAATGAGTTCGTGTATCT
  GTATATAGTTAAAAGACCAGAAGAAAATACAACAAAATGTTAATAAGGAATATCAGGAGTGGAGGGATTA
  TGGGTAATTTTTAATTTCTTCTTTATATCTTTCTGAATTTTCCTATATTTCTATTATGAACATGTACTAT
  TATTACAATGAGGAAAAAATTTGAGGGTAGACACAATATTGAGAAAAATTCCACTCAGCCTTTTAAAAAC
  AGTATAAATTGTATAAGGACTATATAATGTCATTGCAGAGGATACAAATGATGATGATGATGATGTATAA
  AGGTAAACATGAAGTTCCCCCTTGGCACACCCAGCCACTCCAGCCCTCCCCACACATGCATTCCCTCACT
  GGCAGTACAACCACTGGTGATTTTTTTTTTTCTGAGACAGGTTCTTGCTCTGTTTCCCAGGCTGGAGTGC
  AGTGGCACAATCACACCTTACTGCAGCCTCCAACTCCTGGGCTCGAGCAATCCTCCTGCCTCAGCCTCCC
  AAGTAGCTAGGACTATAGGCACACATCACCACACTCAGCTAATTTTTGTATTTCTGGTAGAAATGCAGTC
  TTACTGTATTGCCGAGGGTTGTCTTGAACTTCTGGCCTCAAGCAGTCCTCCCACTTTGGCCTCACCAGAT
  GCTGGGATTACAGGCATGAGCCACTGTGCCCAGCACCACTGGTAATTAATGGCTTGGGGTCCTTCCTTCT
  AATGCCCTTCCAAGTGAGCATGTATTAATTAGGAGTTTTATACCACATACTGCCAGGCAAATATACAAAC
  TGAGTTATGTTGTTTCTTTATTTTTTATTATGCCAATAACAATCTTTCTTTTCTAATATTATTTACTTCA
  GTGCTCCAGAACCTGGAACTCAAGATGCCAGCATCCCAGTTGAGAAACTTCCCCGGTAGGTAGAAAACTC
  TGATTTGTACTGGTGTTCCAGGTTACCAAAACAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC035196 unedited
  NAGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTCTT
  TCAGCTTTTCTCACTCTTTGAGGTGGCCTCCCTTCTCTCTCTGCAATTGATCTGTCTCCA
  CGCGCCAAGGGAGAGTGCCTCCGACGGCTCCAGCTCAGCCACCTGCTCAGAAAATAAACC
  TCTGGGTAGGCTTGCTTGGTCCACACGCCCACCCTTCTGACCAAACACGACTGCAGAGGG
  ATGGACACCACATGGGGGCCGTGCGCAGCATGTGGCTGAGCTTGTATTTGGGATGTGACC
  CACAGCCCTATTGGCTCAGCATGCGAGAGAGCAGGGATGGTGCTCCCCAAGGAAGGACTG
  GTGGGCAGGACGGAGCAGCGCATGTCCACTAGGGTTGAAAGTGCTCTGCACGGCACCAGT
  TGCCTGGTACCCATTTCATAAACTGTGGCCACCATGATTGCTTCAGCTCAATAAGTGGTG
  GTTAGTATTAACAATTACTTTTTTCACTTATTAGTTGATTACTTATTTATTGAGGTGCTT
  TTGTATAAAAGATTCAAGCTTTCTGAAATCGAAGTTAAAATTTTGTAAACATATCCCACG
  CCATTGAACCTTTGTCAGTCATGTCAATAAATCACGCCCTTAGATTTAGGTTGAGCTCCC
  TCTACCCTATTGGCAACTTCTGCCCACTTGAACACCACTCCAAGGTCATCTGATGGCACA
  TTAGTCATGGAGTTATTTATTTTTCCTCCAGATTATAAAGTCCTTGNAGGCATATGCTCT
  TGACTGGCTTAAGATACTGCATGCCTCTNGTTCCCCATGAAGCTAAAGCTAGGGTAGGCC
  CATTTGCTAAAATGAAGTATGGCTCAGTGTGGCAGTGCACACCTATAGTCCCAGCTGCTC
  AGGAGGGGCAAGTGNNGAGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC035196 unedited
  ATTTACTGTGNACCGCGCGCCGTTATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTAA
  TCTATGAACATTCTGTTTGGTCCTGTAACCTCTCCTATTCAGGTCCTTTCATTGCCTCCC
  TAACCCTGGATTAATAATGCCAGTGATGTTATTTGACCAGTTACAGGTCAGCCCCTATGC
  TTTCTGTACACTGGGTAACCACCTGTCGCACGACAGCTTCAGAAACTTTCCCGACCTCAA
  ATGATCCTACCACAACACAAAGCAAAAAGAGGTTGGAAAAAAATCATTCTTTTCATCCTA
  ATTTGGTTCATCCTTTGTCCTTTCACTTTTGGTCTCAGAGATCAAGCCTGGTTTTTACAT
  TTGTGCATTTGATTTAAGAGGCGTCTTGCCAAATACATGTTTAGGTTTTGATGTCTGTTT
  TTTTTTTTGTTTTGGTAACCTGGAACACCAGTACAAATCAGAGTTTTCTACCTACCGGGG
  AAGTTTCTCAACTGGGATGCTGGCATCTTGAGTTCCAGGTTCTGGAGCACTGAAGTAAAT
  AATATTAAAAAAGAAAGATTGTTATTGGCATAATTAAAAATAAAGAAACAACCTAACTTC
  ATTTGTATATTTTCCTGGGAGTATGTGGGATAAAACTCCTAATTAAACATGCTCACTTGG
  AAGGGCATTATAAGGGAAGGACCCCACGCCACTAATTACCCGTGGGTGCTGGGCACAAAT
  GGGCTCATGCCTGGTAATTCCCCCCTTTTGGTGAGGCCACAATGGGGAGGACTTGCCTGG
  AGCCACAAATTTCAAAAAAACCCCTCGCCAAATCAGTAAAATTGGCTTTTTCACCCGAAA
  AACAAAAATTAGCTGAATGCGGCGAAGGTGGCCCCAAAGGCCAAATACCCTGGTAGACCG
  ACAGCAGAAGATATCTTCCCGCCCCCAATTGTGT
  
• Restriction Sites: NotI-NotI
• ACCN: BC035196
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC035196.1
• RefSeq Size: 2428 bp
• RefSeq ORF: 2428 bp
• Locus ID: 23408
• Cytogenetics: 6p23
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]