C9orf72 (AK001971) Human Untagged Clone

CAT#: SC102059

(untagged)-Human cDNA FLJ11109 fis, clone PLACE1005851

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C9orf72
• Synonyms: ALSFTD; FTDALS; FTDALS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK001971, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCACCCTGTCATGAACATATTTATAATCAGCGTAGATACATGAGATCCGAGCTGACAGCCTTCTGGA
  GAGCCACTTCAGAAGAAGACATGGCTCAGGATACGATCATCTACACTGACGAAAGCTTTACTCCTGATTT
  GAATATTTTTCAAGATGTCTTACACAGAGACACTCTAGTGAAAGCCTTCCTGGATCAGGTCTTTCAGCTG
  AAACCTGGCTTATCTCTCAGAAGTACTTTCCTTGCACAGTTTCTACTTGTCCTTCACAGAAAAGCCTTGA
  CACTAATAAAATATATAGAAGACGATACGCAGAAGGGAAAAAAGCCCTTTAAATCTCTTCGGAACCTGAA
  GATAGACCTTGATTTAACAGCAGAGGGCGATCTTAACATAATAATGGCTCTGGCTGAGAAAATTAAACCA
  GGCCTACACTCTTTTATCTTTGGAAGACCTTTCTACACTAGTGTGCAAGAACGAGATGTTCTAATGACTT
  TTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK001971 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTTTCTGACTCCAGCAG
  AGAGAAAATGCTCCAGGTTATGTGAAGCAGAATCATCATTTAAATATGAGTCAGGGCTCT
  TTGTACAAGGCCTGCTAAAGGATTCAACTGGAAGCTTTGTGCTGCCTTTCCGGCAAGTCA
  TGTATGCTCCATATCCCACCACACACATAGATGTGGATGTCAATACTGTGAAGCAGATGC
  CACCCTGTCATGAACATATTTATAATCAGCGTAGATACATGAGATCCGAGCTGACAGCCT
  TCTGGAGAGCCACTTCAGAAGAAGACATGGCTCAGGATACGATCATCTACACTGACGAAA
  GCTTTACTCCTGATTTGAATATTTTTCAAGATGTCTTACACAGAGACACTCTAGTGAAAG
  CCTTCCTGGATCAGGTCTTTCAGCTGAAACCTGGCTTATCTCTCAGAAGTACTTTCCTTG
  CACAGTTTCTACTTGTCCTTCACAGAAAAGCCTTGACACTAATAAAATATATAGAAGACG
  ATACAAGGGAAAAAAGCCCTTTAAATCTCTTCGGAACCTGAAGATAGACCTTGATTTAAC
  AGCAGAGGGCGATCTTAACATAATAATGGCTCTGGCTGAGAAAATTAAACCAGGCCTACA
  CTCTNTTATCTTTGGAAGACCTTTCTACACTAGTGTGCAAGAACGAGATGTTCTAATGAC
  TNTNTAAATGTGTAACTTAATAAGCCTATTCCATCACAATCATGATCGCTGGTAAAGTAG
  CTCAGTGGTGTGGNGAAAACGTTCCCCTGGATCATACTCCAGAATNCTGCTCTCAGCATT
  TGCAGTAAGTAAGTACACTACAGNTCTCACAGAGCCTGTGAAGGGAATGTCAGTGCATCA
  TAACTTNGGNNGNCTCTTTCCTAGATAATGCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK001971 unedited
  GTACAACTATGNNACCGCGGCCGCATACTAGNGATCGATTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTCAAAAATTTCAAATATTTTTTTATCCAAAATTTCTCCAT
  TTAGGAAAAAAAGATTTTTAACAATGTTTACCCATGCCTTTAATAACTTATTTCACTGGC
  CAACTTACATTCTGTATAACAGTCCAATAAACCACCCAAAAAAAATAACCGGTTAGCCCT
  TAAATAAAAATCTCCCATGTAAAAAACACAGTTTGGGACACTACAGGGGAGAATTTATAT
  ATTTTTAATAGGACTGAGCTACAGTACAACAGTCATTTAGTTCAGGGGTTGTCTAAAACA
  TCAAGCTGTCCACATTTTTTTGATTCATGAGGGGAAAGCTATTATGACCTTTCACATTCA
  AACATGTCATTTTGTTGGGAAAATTTGGGGGGGGGGGGGCAAAAGGGCTCTATTACCTTT
  ATCCCTTTCTTATAAATATATTTTCCCTTTTATATTACTTCCAAAATTTTAAATAAAATA
  TTTATTTGGGTTTGGGAAACTACAATGTCAACATTGGGTTAAAATTATATTAATCAGTTA
  TTTTATACCCAGCAAAATGGTCCAAACCCTTTAAAAAAACAAAAAATAACTTACCTCTGC
  CAAAGTACCACCTAGGAAAACAGCACTTCAGNAAAATTTCTCCGGCTATTCCTTATAGCC
  TATTGCAGGCAAACAGCAACAACTTCAAAAACATAGGCAGAAATGCAAGAAGGAAGTCCT
  CCAGGAACTATTTTCTGTTTCTGAAAAAAAGATCTTATTAGTTAGTATATTCTCTAAGCC
  ATTTTATAATCTCAAAGTTGCAATGATTGCCAAAGCGGTACCTGTTAAGACTTTCCNNGA
  GAAAAACGGATGAAAATGCCTGCCCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: AK001971
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK001971.1, BAA92009.1
• RefSeq Size: 2185 bp
• RefSeq ORF: 495 bp
• Locus ID: 203228
• Cytogenetics: 9p21.2
• Gene Summary: The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016]