GPR114 (ADGRG5) (NM_153837) Human Untagged Clone

CAT#: SC100887

GPR114 (untagged)-Human G protein-coupled receptor 114 (GPR114)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR114
• Synonyms: GPR114; PGR27
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100887 sequence for NM_153837 edited (data generated by NextGen Sequencing)
  ATGGATCACTGTGGTGCCCTTTTCCTGTGCCTGTGCCTTCTGACTTTGCAGAATGCAACA
  ACAGAGACATGGGAAGAACTCCTGAGCTACATGGAGAATATGCAGGTGTCCAGGGGCCGG
  AGCTCAGTTTTTTCCTCTCGTCAACTCCACCAGCTGGAGCAGATGCTACTGAACACCAGC
  TTCCCAGGCTACAACCTGACCTTGCAGACACCCACCATCCAGTCTCTGGCCTTCAAGCTG
  AGCTGTGACTTCTCTGGCCTCTCGCTGACCAGTGCCACTCTGAAGCGGGTGCCCCAGGCA
  GGAGGTCAGCATGCCCGGGGTCAGCACGCCATGCAGTTCCCCGCCGAGCTGACCCGGGAC
  GCCTGCAAGACCCGCCCCAGGGAGCTGCGGCTCATCTGTATCTACTTCTCCAACACCCAC
  TTTTTCAAGGATGAAAACAACTCATCTCTGCTGAATAACTACGTCCTGGGGGCCCAGCTG
  AGTCATGGGCACGTGAACAACCTCAGGGATCCTGTGAACATCAGCTTCTGGCACAACCAA
  AGCCTGGAAGGCTACACCCTGACCTGTGTCTTCTGGAAGGAGGGAGCCAGGAAACAGCCC
  TGGGGGGGCTGGAGCCCTGAGGGCTGTCGTACAGAGCAGCCCTCCCACTCTCAGGTGCTC
  TGCCGCTGCAACCACCTCACCTACTTTGCTGTTCTCATGCAACTCTCCCCAGCCCTGGTC
  CCTGCAGAGTTGCTGGCACCTCTTACGTACATCTCCCTCGTGGGCTGCAGCATCTCCATC
  GTGGCCTCGCTGATCACAGTCCTGCTGCACTTCCATTTCAGGAAGCAGAGTGACTCCTTA
  ACACGCATCCACATGAACCTGCATGCCTCCGTGCTGCTCCTGAACATCGCCTTCCTGCTG
  AGCCCCGCATTCGCAATGTCTCCTGTGCCCGGGTCAGCATGCACGGCTCTGGCCGCTGCC
  CTGCACTACGCGCTGCTCAGCTGCCTCACCTGGATGGCCATCGAGGGCTTCAACCTCTAC
  CTCCTCCTCGGGCGTGTCTACAACATCTACATCCGCAGATATGTGTTCAAGCTTGGTGTG
  CTAGGCTGGGGGGCCCCAGCCCTCCTGGTGCTGCTTTCCCTCTCTGTCAAGAGCTCGGTA
  TACGGACCCTGCACAATCCCCGTCTTCGACAGCTGGGAGAATGGCACAGGCTTCCAGAAC
  ATGTCCATATGCTGGGTGCGGAGCCCCGTGGTGCACAGTGTCCTGGTCATGGGCTACGGC
  GGCCTCACGTCCCTCTTCAACCTGGTGGTGCTGGCCTGGGCGCTGTGGACCCTGCGCAGG
  CTGCGGGAGCGGGCGGATGCACCAAGTGTCAGGGCCTGCCATGACACTGTCACTGTGCTG
  GGCCTCACCGTGCTGCTGGGAACCACCTGGGCCTTGGCCTTCTTTTCTTTTGGCGTCTTC
  CTGCTGCCCCAGCTGTTCCTCTTCACCATCTTAAACTCGCTCTACGGTTTCTTCCTTTTC
  CTGTGGTTCTGCTCCCAGCGGTGCCGCTCAGAAGCAGAGGCCAAGGCACAGATAGAGGCC
  TTCAGCTCCTCCCAAACAACACAGTAG
  
  Clone variation with respect to NM_153837.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153837 unedited
  TGCGGCATTTGTNAAACGACTCACTATAGGCGGCCGCGNAATTCGCTTTCTGCACAGGCA
  TGGATCACTGTGGTGCCCTTTTCCTGTGCCTGTGCCTTCTGACTTTGCAGNAATGCAACA
  ACAGAGACATGGGAAGAACTCCTGAGCTACATGGGAGAATATGCAGGTGTCCAGGGGCCG
  GAGCTCAGTTTTTTCCTCTCGTCAACTCCACCAGCTGGAGCAGATGCTACTGAACACCAG
  CTTCCCAGGCTACAACCTGACCTTGCAGACACCCACCATCCAGTCTCTGGCCTTCAAGCT
  GAGCTGTGACTTCTCTGGCCTCTCGCTGACCAGTGCCACTCTGAAGCGGGTGCCCCAGGC
  AGGAGGTCAGCATGCCCGGGGTCAGCACGCCATGCAGTTCCCCGCCGAGCTGACCCGGGA
  CGCCTGCAAGACCCGCCCCAGGGAGCTGCGGCTCATCTGTATCTACTTCTCCAACACCCA
  CTTTTTCAAGGATGAAAACAACTCATCTCTGCTGAATAACTACGTCCTGGGGGCCCAGCT
  GAGTCATGGGCACGTGAACAACCTCAGGGATCCTGTGAACATCAGCTTCTGGCACAACCA
  AAGCCTGGAAGGCTACACCCTGACCTGTGTCTTCTGGAAGGAGGGAGCCAGGAAACAGCC
  CTGGGGGGGCTGGAGCCCTGAGGGCTGTCGTACAGAGCAGCCCTCCCACTCTCAGGTGCT
  CTGCCGCTGCAACCACCTCACCTACTTTGCTGGTCTCATGCAACTCTCCCCACCCTGGTC
  CCTGCAGAGTTGCTGGCACCTCTTACGTACATCTCCCTCGTGGGCTGCAGCATCTACATC
  GTGGCCTCGCTGATCACAGTCCTGCTGCACTTCCATTTCAGGAAGCAGAGTGACTTCTTA
  ACACGCATCCACATGAAACCTGCATGCCTGCGTGCTGATCCTGAAATCGACTTACTGGTG
  AGCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153837 unedited
  ATAAGGCTCTTGGCGATGGTCAACTTCCCATGNCCAGGAAAGCACTGGGGAAGGGTCACA
  GGGCTGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTATAGT
  CGACAAGCTTGATATCGGTACCAGATCTGAATTCGGCTTGGACTACTGTGTTGTTTGGGA
  GGAGCTGAAGGCCTCTATCTGTGCCTTGGCCTCTGCTTCTGAGCGGCACCGCTGGGAGCA
  GAACCACAGGAAAAGGAAGAAACCGTAGAGCGAGTTTAAGATGGTGAAGAGGAACAGCTG
  GGGCAGCAGGAAGACGCCAAAAGAAAAGAAGGCCAAGGCCCATGTGGTTCCCAGCAGCAC
  GGTGAGGCCCAGCACAGTGACAGTGTCATGGCAGGCCCTGACACTTGGTGCATCCGCCCG
  CTCCCGCAGCCTGCGCAGGGTCCACAGCGCCCAGGCCAGCACCACCAGGTTGAAGAGGGA
  CGTGAGGCCGCCGTAGCCCATGACCAGGACACTGTGCACCACGGGGCTCCGCACCCAGCA
  TATGGACATGTTCTGGAAGCCTGTGCCATTCTCCCAGCTGTCGAAGACGGGGATTGTGCA
  GGGTCCGTATACCGAGCTCTTGACAGAGAGGGAAAGCAGCACCAGGAGGGCTGGGGCCCC
  CCAGCCTAGCACACCAAGCTTGAACACATATCTGCGGATGTAGATGTTGTAGACACGCCC
  GAAGAGGAGGTAGAGGTTGAAGCCCTCGATGGCCATCCAGGTGAGGCAGCTGATCAGCGC
  GTAGTGCAGGGCAGCGGCCAGAGCCGTGCATGCTGACCCGGGCACAGGATACATTGCGAA
  TGCGGAGCTCANNCAGAATGCGATGTTCAAGAGCAGCACGGNAGCATGCATGTTCATGTG
  GGT
  
• Restriction Sites: Please inquire
• ACCN: NM_153837
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153837.1, NP_722579.1
• RefSeq Size: 3848 bp
• RefSeq ORF: 1587 bp
• Locus ID: 221188
• UniProt ID: Q8IZF4
• Cytogenetics: 16q21
• Domains: GPS, 7tm_2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene encodes a member of the adhesion family of G-protein coupled receptors. Members of this family are characterized by long N-termini and multiple functional domains. They may play a role in the immune system as well as in the central nervous system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode the same isoform (1).