BRRN1 (NCAPH) (NM_015341) Human Untagged Clone

CAT#: SC100677

NCAPH (untagged)-Human non-SMC condensin I complex, subunit H (NCAPH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCAPH
• Synonyms: BRRN1; CAP-H; CAPH; MCPH23
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015341, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGACCTCCCGGCCCAGCACTGCCAGCCACAATGAATAACTCTTCTTCAGAGACGCGAGGACACCCCC
  ACAGTGCCTCCTCTCCTTCAGAGCGTGTGTTCCCGATGCCCCTGCCCAGGAAGGCGCCTCTCAATATTCC
  TGGCACCCCAGTCCTCGAAGACTTTCCTCAGAATGACGATGAGAAGGAGCGGCTGCAGCGGAGGCGCTCG
  AGGGTCTTTGATCTGCAGTTCAGCACTGACTCACCTCGCTTATTGGCCTCCCCCTCCAGCAGGAGTATTG
  ACATTTCAGCTACTATCCCCAAGTTTACAAACACGCAGATTACGGAACATTACTCCACCTGTATCAAACT
  GTCCACTGAAAATAAAATCACTACCAAGAATGCTTTTGGTTTGCACTTGATTGATTTTATGTCAGAGATT
  CTTAAACAGAAAGACACCGAACCAACCAACTTTAAAGTGGCTGCGGGTACTCTGGATGCCAGCACCAAGA
  TCTATGCTGTGCGCGTGGATGCCGTCCATGCCGATGTATACAGAGTCCTTGGGGGGCTGGGCAAAGATGC
  ACCGTCTTTGGAAGAAGTAGAAGGCCATGTTGCTGATGGAAGTGCTACTGAAATGGGAACAACCAAAAAG
  GCTGTAAAGCCAAAGAAGAAGCACTTACACAGAACTATTGAGCAGAACATAAACAACCTCAATGTCTCCG
  AAGCAGATCGGAAGTGTGAGATTGATCCCATGTTTCAGAAGACAGCAGCCTCATTTGATGAGTGCAGCAC
  AGCAGGGGTGTTTCTGTCCACTCTCCACTGCCAGGACTACAGAAGTGAACTGCTGTTTCCCTCTGATGTC
  CAGACTCTCTCCACGGGAGAACCTCTCGAGTTGCCAGAGTTAGGTTGTGTAGAAATGACAGATTTAAAAG
  CGCCCTTGCAGCAGTGTGCAGAAGATCGCCAGATCTGCCCTTCCCTGGCCGGGTTCCAGTTTACACAGTG
  GGACAGTGAAACACATAATGAGTCTGTGTCGGCCCTGGTAGACAAGTTTAAGAAGAATGACCAGGTATTT
  GACATCAATGCTGAAGTTGACGAGAGTGACTGTGGAGACTTCCCCGATGGGTCCCTGGGGGATGACTTTG
  ATGCCAACGATGAACCTGACCACACCGCAGTTGGGGATCATGAAGAGTTCAGGAGCTGGAAGGAGCCCTG
  CCAGGTTCAGAGCTGCCAGGAAGAAATGATTTCCCTTGGGGATGGAGACATCAGGACCATGTGCCCCCTT
  CTGTCTATGAAACCTGGAGAATATTCTTATTTCAGTCCTCGGACCATGTCGATGTGGGCTGGCCCGGATC
  ACTGGCGCTTTAGGCCTCGACGCAAACAAGATGCTCCTTCCCAATCAGAAAACAAAAAGAAGAGTACAAA
  AAAAGATTTTGAAATTGACTTTGAAGATGATATTGACTTTGATGTATATTTTAGAAAAACAAAGGCTGCT
  ACTATTCTGACCAAGTCCACTTTGGAGAACCAGAATTGGAGAGCTACCACCCTTCCTACAGATTTCAACT
  ACAATGTTGACACTCTGGTCCAGCTTCACCTCAAACCAGGCACCAGGTTACTTAAGATGGCCCAGGGCCA
  TAGGGTAGAGACTGAGCATTATGAAGAAATTGAAGACTATGATTACAACAACCCTAACGACACCTCCAAC
  TTTTGCCCTGGATTACAGGCTGCTGACAGTGATGATGAAGATTTGGATGACTTATTTGTGGGACCTGTTG
  GGAACTCTGACCTCTCACCTTATCCTTGCCATCCACCTAAGACAGCACAACAGAATGGTGACACTCCAGA
  AGCCCAAGGATTAGACATCACAACATATGGGGAGTCAAACTTGGTAGCTGAGCCTCAGAAGGTAAATAAA
  ATTGAAATTCACTATGCCAAGACTGCCAAAAAGATGGACATGAAGAAACTGAAGCAGAGCATGTGGAGTC
  TGCTGACAGCGCTCTCCGGAAAGGAGGCAGATGCAGAGGCAAACCACAGGGAAGCTGGAAAAGAAGCGGC
  CCTGGCAGAAGTGGCTGACGAGAAGATGCTTAGCGGGCTCACGAAGGACCTGCAGAGGAGCCTGCCCCCT
  GTCATGGCTCAGAACCTCTCCATACCTCTGGCTTTTGCCTGTCTCCTACATTTAGCCAATGAAAAGAATC
  TAAAACTGGAAGGAACAGAGGACCTCTCTGATGTTCTTGTGAGGCAAGGAGATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015341 unedited
  ACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGCCAAGGAAAGAGGGACCTCCCGGC
  CCAGCACTGCCAGCCACAATGAATAACTCTTCTTCAGAGACGCGAGGACACCCCCACAGT
  GCCTCCTCTCCTTCAGAGCGTGTGTTCCCGATGCCCCTGCCCAGGAAGGCGCCTCTCAAT
  ATTCCTGGCACCCCAGTCCTCGAAGACTTTCCTCAGAATGACGATGAGAAGGAGCGGCTG
  CAGCGGAGGCGCTCGAGGGTCTTTGATCTGCAGTTCAGCACTGACTCACCTCGCTTATTG
  GCCTCCCCCTCCAGCAGGAGTATTGACATTTCAGCTACTATCCCCAAGTTTACAAACACG
  CAGATTACGGAACATTACTCCACCTGTATCAAACTGTCCACTGAAAATAAAATCACTACC
  AAGAATGCTTTTGGTTTGCACTTGATTGATTTTATGTCAGAGATTCTTAAACAGAAAGAC
  ACCGAACCAACCAACTTTAAAGTGGCTGCGGGTACTCTGGATGCCAGCACCAAGATCTAT
  GCTGTGCGCGTGGATGCCGTCCATGCCGATGTATACAGAGTCCTTGGGGGGCTGGGCAAA
  GATGCACCGTCTTTGGAAGAAGTAGAAGGCCATGTTGCTGATGGAAGTGCTACTGAAATG
  GGAACAACCAAAAAGGCTGTAAAGCCAAAGAANAAGCACTTACACAGAACTATTGAGCAG
  AACATANACAACCTCAATGTCTCCGAAGCAGATCGGAGTGTGAGATTGATCCCATGGTTC
  AGAAGACACAGCCTCTTTGATGAGTGCAGCCAGCAGGGGGGGTTCTGTCACTCTTCATGC
  CCAGATACGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015341
• Insert Size: 4130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015341.2, NP_056156.2
• RefSeq Size: 6059 bp
• RefSeq ORF: 2226 bp
• Locus ID: 23397
• UniProt ID: Q15003
• Cytogenetics: 2q11.2
• Gene Summary: This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.