HIBADH (NM_152740) Human Untagged Clone

CAT#: SC100318

HIBADH (untagged)-Human 3-hydroxyisobutyrate dehydrogenase (HIBADH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIBADH
• Synonyms: NS5ATP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100318 sequence for NM_152740 edited (data generated by NextGen Sequencing)
  ATGGCAGCCTCCTTACGGCTCCTCGGAGCTGCCTCCGGTCTCCGGTACTGGAGCCGGCGG
  CTGCGGCCGGCAGCCGGCAGCTTTGCAGCGGTGTGTTCTAGGTCAGTGGCTTCAAAGACT
  CCAGTTGGATTCATTGGACTGGGCAACATGGGGAATCCAATGGCAAAAAATCTCATGAAA
  CATGGCTATCCACTTATTATTTATGATGTGTTCCCTGATGCCTGCAAAGAGTTTCAAGAT
  GCAGGTGAACAGGTAGTATCTTCCCCAGCAGATGTTGCTGAAAAAGCTGACAGAATTATT
  ACAATGCTGCCCACCAGTATCAATGCAATAGAAGCTTATTCCGGAGCAAATGGGATTCTA
  AAAAAAGTGAAGAAGGGCTCATTATTAATAGATTCCAGCACTATTGATCCTGCAGTTTCA
  AAAGAATTGGCCAAAGAAGTTGAGAAAATGGGAGCAGTTTTCATGGATGCCCCTGTTTCT
  GGTGGTGTAGGAGCTGCACGATCTGGGAACCTCACGTTTATGGTGGGAGGAGTTGAAGAT
  GAATTTGCTGCTGCCCAAGAGTTGCTGGGGTGCATGGGCTCCAACGTGGTGTACTGTGGA
  GCTGTTGGGACTGGGCAGGCGGCAAAGATCTGCAACAACATGCTGTTAGCTATTAGTATG
  ATTGGAACTGCTGAAGCTATGAATCTTGGAATCAGGTTAGGGCTTGACCCAAAACTACTG
  GCTAAAATCCTAAATATGAGCTCAGGACGGTGTTGGTCAAGTGACACTTATAATCCTGTA
  CCTGGAGTGATGGATGGCGTTCCCTCGGCTAATAACTATCAGGGTGGATTTGGAACAACA
  CTCATGGCTAAGGATCTGGGATTGGCACAAGACTCTGCTACCAGCACAAAGAGCCCAATC
  CTTCTTGGCAGTCTGGCCCATCAGATCTACAGGATGATGTGTGCAAAGGGCTACTCAAAG
  AAAGACTTCTCATCCGTGTTCCAGTTCCTACGAGAGGAGGAGACCTTCTGA
  
  Clone variation with respect to NM_152740.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152740 unedited
  ACAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCGCGCCCTTC
  TCCGTGGGCGAGCCAGCCAGTCCCGCTGCACACGCTCGCAGTCTGTGGGCCCTCCGGGAG
  GCGGCGGAGGTCACCGCGGGGAGAGGGGCGGGCGCAGCATGGCAGCCTCCTTACGGCTCC
  TCGGAGCTGCCTCCGGTCTCCGGTACTGGAGCCGGCGGCTGCGGCCGGCAGCCGGCAGCT
  TTGCAGCGGTGTGTTCTAGGTCAGTGGCTTCAAAGACTCCAGTTGGATTCATTGGACTGG
  GCAACATGGGGAATCCAATGGCAAAAAATCTCATGAAACATGGCTATCCACTTATTATTT
  ATGATGTGTTCCCTGATGCCTGCAAAGAGTTTCAAGATGCAGGTGAACAGGTAGTATCTT
  CCCCAGCAGATGTTGCTGAAAAAGCTGACAGAATTATTACAATGCTGCCCACCAGTATCA
  ATGCAATAGAAGCTTATTCCGGAGCAAATGGGATTCTAAAAAAAGTGAAGAAGGGCTCAT
  TATTAATAGATTCCAGCACTATTGATCCTGCAGTTTCAAAAGAATTGGCCAAAGAAGTTG
  AGAAAATGGGAGCAGTTTTCATGGATGCCCCTGTTTCTGGTGGTGTAGGAGCTGCACGAT
  CTGGGAACCTCACGTTTATGGTGGGAGGAGTTGAAGATGAATTTGCTGCTGCCCAAGAGT
  TGCTGGGGTGCATGGGCTCCAACGTGGTGTACTGTGGAGCTGTTGGGACTGGGCANGCGG
  CANAGATCTGCAACACATGCTGNTAGCTATTAGTATGATTGGAACTGCTGAAGCTATGAT
  CTGGAATCANGTTAGGCTGACCCAAACTACTGGCTAAATCCTAATATGAGCTCNNGACGT
  GTGGTCAGTGACACTATATNCTGTACTGNATGATGGATGGCGTCCTCGCTATACTATAGG
  TGGGATTGACACCTCAGNCTAGACTGGATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152740 unedited
  NAATTTAGCTATGNACCGCGGCCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTT
  TTTTTACCAGTATAGAAATTTATTTGAATTCAAAATAATATGGCTATATTTATAATAATA
  TAATAATTATCTAAAGCAAATATCATCATTGGCTCTGAAATGCGTCTAAAGATGTCATTC
  TTAAGTCAAAAAATACGTAGTAAGAATGTACAAGAAAGAAAAAAATATAAAAACAAGTCT
  GCTGAGTGTCGGGAGTTGGTGAGGGATATCCTACCATATTGTGACGGAGTCCAAATAGAA
  AACATGCAGCAACAGTTCTCCTGCTTTATCAGCTCCCTGGAAAATAAACCAGTAACCCTG
  GTAGTGCAGTAACCATTTGGTTAACAGGACAAACTTCCTGATGGACACAGATAGTAATTC
  ACTGCATTTCCCTTCTCTAACTTCTCTCTTCACACCAATTCCTTTTCTTTCCTTTAAGAT
  GGGTTTCATCCTGTTGACAAAAGATTTGGTTTTATTTGTAAAGTAAAGCAGATAATATCC
  TGATTGAAGTATTCAATGATTTAGTTGAGGATGCTTGGGGATCAAACTTTGTAAAAAGGT
  CAATTAAGCTAGTTAGCAGAGACTATCAGTGGCTTGCAGAAAAAAAATTCGGATAATATG
  TTTGTTAAAAAGACAAAAAGAATAAGCGGTGAAAAATCCTAGGGATTACTGTGACCTAGA
  CAATCAAAAGCAGATAGGTGACCTTTGATTAAATCCATTTNACTTGTGGAGTGAGCTAAA
  AGGAGGCTCCAAGACAGAGTTTGGTTCCCAACAGTGTCCGTGGCCAAAGGGCACACTCAG
  AAAGGTCTCTCCTCTNCGTAGACTGGNACACGATGAGAAGTCTTTCCTTGAGTAGCCTTT
  GCACACTCATCCTGTAGATCTGATGGGCCNAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152740
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152740.2, NP_689953.1
• RefSeq Size: 2006 bp
• RefSeq ORF: 1011 bp
• Locus ID: 11112
• UniProt ID: P31937
• Cytogenetics: 7p15.2
• Domains: NAD_binding_2
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011]