TRIP12 (NM_001284215) Human Tagged ORF Clone

CAT#: RG240227

TRIP12 (tGFP-tagged) - Human thyroid hormone receptor interactor 12 (TRIP12), transcript variant 2

Product Images

Circular map for RG240227

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TRIP12
• Synonyms: MRD49; TRIP-12; TRIPC; ULF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240227 representing NM_001284215.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCC
  CAACCACAAGACGACTCAATAGGAGGAAGAAGCTGCAGTTCATCATCTGCTGTGATAGTTCCACAACCA
  GAGGATCCAGACAGAGCCAATACTTCAGAAAGACAAAAAACGGGGCAGGTGCCTAAGAAAGACAATTCT
  CGAGGAGTGAAGCGCAGTGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCAAAAAAACCA
  AAAGCACTTCAGCATACTGAATCTCCCTCAGAAACAAATAAGCCACATAGTAAGTCAAAGAAGAGACAT
  TTAGACCAGGAGCAACAACTGAAATCTGCACAATCACCATCAACAAGCAAGGCTCATACCAGGAAGAGT
  GGGGCCACTGGCGGTTCACGGAGTCAGAAAAGAAAAAGGACAGAGAGTTCTTGTGTAAAGAGTGGCTCC
  GGGTCTGAATCAACTGGTGCAGAAGAGAGATCTGCGAAACCTACCAAGCTGGCTTCAAAATCAGCCACC
  TCAGCCAAAGCTGGGTGTAGCACCATCACTGATTCTTCTTCTGCTGCCTCTACTTCCTCCTCGTCTTCT
  GCTGTAGCCTCGGCCTCCTCCACTGTACCACCAGGTGCCAGAGTGAAACAAGGAAAAGATCAGAACAAG
  GCCAGGCGTTCCCGTTCAGCGTCCAGTCCCAGCCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAA
  ACTGGTGGCTCTTCAAAATTTGATTGGGCTGCTCGTTTCAGCCCTAAAGTTAGCCTTCCTAAAACAAAA
  CTGTCTCTTCCAGGGTCTTCTAAGTCAGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTA
  GCAAGTTTAAGAAAATCTACGAAGAAACGCAGTGAGTCTCCACCTGCTGAGCTCCCCAGTTTGAGGCGG
  AGCACACGCCAAAAGACCACGGGCTCCTGTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAAAGA
  GGAGCAGCTGAAGCTCGTCGACAGGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCT
  TCAGCTGCTCGGACAGATGAAGCTCCCCAAGGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGC
  ATGACCACCTCTGGGGAGAGTGAATCAGATGATTCCGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCA
  AGGGGTCTTCCCCCTCACCTATTTGGTCCTCTTGGTCCTCGGATGTCACAGCTTTTCCATAGAACAATT
  GGAAGTGGAGCTAGTTCTAAGGCCCAGCAGCTACTACAAGGATTGCAAGCCAGTGATGAAAGTCAACAG
  CTTCAGGCAGTTATTGAGATGTGTCAGTTACTGGTCATGGGAAATGAGGAGACACTGGGAGGGTTTCCT
  GTCAAGAGTGTTGTTCCAGCTTTGATTACGTTACTTCAGATGGAGCACAATTTTGATATTATGAACCAT
  GCTTGTCGAGCCTTAACATACATGATGGAAGCACTTCCTCGATCTTCTGCTGTTGTAGTAGATGCTATT
  CCTGTCTTTTTAGAAAAGCTGCAAGTTATTCAGTGTATTGATGTGGCAGAGCAGGCCTTGACTGCCTTG
  GAGATGTTGTCACGGAGACATAGTAAAGCCATTCTACAGGCGGGTGGTTTGGCAGACTGCTTGCTGTAC
  CTAGAATTCTTCAGCATAAATGCCCAAAGAAATGCATTAGCAATTGCAGCTAATTGCTGCCAGAGTATC
  ACGCCAGATGAATTTCATTTTGTGGCAGATTCACTCCCATTGCTAACCCAAAGGCTAACACATCAGGAT
  AAAAAGTCAGTAGAAAGCACTTGCCTTTGTTTTGCACGCCTAGTGGACAACTTCCAGCATGAGGAGAAT
  TTACTCCAGCAGGTTGCTTCCAAAGATCTGCTTACAAATGTTCAACAGCTGTTGGTAGTGACTCCACCC
  ATTTTAAGTTCTGGGATGTTTATAATGGTGGTTCGCATGTTTTCTCTGATGTGTTCCAACTGTCCAACT
  TTAGCTGTTCAACTTATGAAACAAAACATTGCAGAAACGCTTCACTTTCTCCTGTGTGGTGCCTCCAAT
  GGAAGTTGTCAGGAACAGATTGATCTTGTTCCACGAAGCCCTCAAGAGTTGTATGAACTGACATCTCTG
  ATTTGTGAACTTATGCCATGTTTACCAAAAGAAGGCATTTTTGCAGTTGATACCATGTTGAAGAAGGGA
  AATGCACAGAACACAGATGGTGCGATATGGCAGTGGCGTGATGATCGGGGCCTCTGGCATCCATATAAC
  AGGATTGACAGCCGGATCATTGAGGCAGCCCATCAGGTCGGTGAGGATGAGATAAGCTTGTCCACTCTG
  GGACGAGTTTATACTATTGATTTTAATTCTATGCAGCAAATCAATGAGGACACGGGAACAGCACGTGCC
  ATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAACACTAGTGGATATTCAGAGTCAAAGAAGGATGAT
  GCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTGGCTAAGTCTTTTATTAAGACATTATTTGGTGTT
  CTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGGTCAGACATAAGTGCCTTAGAGCAATTCTTAGG
  ATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGTTCTGAAAAATCATGCTGTTTCAAGTCACATT
  GCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTGGGAGCACTTCAGATGGCAGAAATTTTAATG
  CAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAGAAGGTGTAATGCATCAAGTAAAACACTTA
  GCAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATGTACGAATGGATCGGGATCCATGGGATCC
  ACAACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCATGCTGCAGCTGACTTGGGATCACCCAGC
  TTGCAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAGGTCGATTAAGTGATGTTCTAAAGAGA
  AAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACCTCCAAGAGATGATGACAAAGTAGAC
  AATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCTTTCCTGGCAAGCTTGAATCCAAAA
  ACATGGGGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGCCAGCACGGACTGCGGGAGGTAGT
  GGCCTTGCCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGAAAAAATTAAAGGTTGGATTAAG
  GAGCAGGCACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATGGATGGAAGCAACCCTGCATTG
  AATGTCCTTCAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGC
  CTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGAAATCCAACATAGTGGATTT
  GTGAAGCAGCTGTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTGAGCAGAGAGATCAGATTA
  AAGCGATTTCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCATTGGAAGAGTGGAACCA
  GTGGGTAATGCACCTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAGCCAGATGGAACAATTT
  CCAGTCAAAGTACATGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCTCTCAACAGAGGATCA
  CAGGCTTTAAAATTTTTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATCCAGACTGTGCAAAT
  GTGAAGCAGTGGAAGGGTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGCCATCGAGAGATAC
  CTTGTAGTTAGAGGGTATGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGATGGATCAGATGAG
  GAAATAGATGAGTCTCTGGCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGCTGCAGTTTTAT
  ATTGGAGAACATTTGCTGCCGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAGTATACAGGCT
  GAAGATGAAAGAGAATCCACAGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGGACAAAGACT
  CATACAATATGGTATAAACCTGTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTGGTAAAAGA
  GGAAGAGCCCAAACAGCTCCAACGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTTATGGCAC
  GATGGAGTGTGCCCATCAGTATCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATA
  ACATTTGAAGACCCGTCATTAGATGTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGATACTGG
  TATTACTTGTATGATAATGCAATGTGCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAAGTTA
  ACAGCAAAAGCAAATAGGCAACTTCAAGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGGCTT
  ACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAACT
  GCATTTGATCGGGACCGAGCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCT
  CAAGATAGCAGAGTTGCACCTAGATTGGATAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAA
  CAGGCGGAGTCTGTGATGCAGGACCTCGGCAGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAG
  GTTGGTACAGGTCTTGGGCCTACACTGGAGTTTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGAC
  TTGGGTCTTTGGAGAGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTAT
  ATTCAAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAG
  GTTAAGATGAAGTTTCGCTTCTTAGGAAAATTAATGGCCAAGGCTATCATGGATTTCAGATTGGTGGAC
  CTTCCCCTTGGCTTACCCTTTTATAAATGGATGCTACGGCAAGAAACTTCACTGACATCACACGATTTG
  TTTGACATCGACCCAGTTGTAGCCAGATCAGTTTATCACCTAGAAGACATTGTCAGACAGAAGAAAAGA
  CTTGAACAAGATAAATCCCAGACCAAAGAGAGTCTACAGTATGCATTAGAAACCTTGACTATGAATGGC
  TGCTCAGTTGAAGATCTAGGACTGGATTTCACTCTGCCAGGGTTTCCCAATATCGAACTGAAGAAAGGA
  GGGAAGGATATACCAGTCACTATCCACAATTTAGAGGAGTATCTAAGACTGGTTATATTCTGGGCACTA
  AATGAAGGCGTTTCTAGGCAATTTGATTCGTTCAGAGATGGATTTGAATCAGTCTTCCCACTCAGTCAT
  CTTCAGTACTTCTACCCGGAGGAACTGGATCAGCTCCTTTGTGGCAGTAAAGCAGACACTTGGGATGCA
  AAGACACTGATGGAATGCTGTAGGCCTGATCATGGTTATACTCATGACAGTCGGGCTGTGAAGTTTTTG
  TTTGAGATTCTCAGTAGTTTTGATAATGAGCAGCAGAGGTTATTTCTCCAGTTTGTGACTGGTAGCCCA
  AGATTGCCTGTTGGAGGATTCCGGAGTTTGAATCCACCTTTGACAATTGTCCGAAAGACGTTTGAATCA
  ACAGAAAACCCAGATGACTTCTTGCCCTCTGTAATGACTTGTGTGAACTATCTTAAGTTGCCGGACTAT
  TCAAGCATTGAGATAATGCGTGAAAAACTGTTGATAGCAGCAAGAGAAGGGCAGCAGTCGTTCCATCTT
  TCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240227
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNS
  RGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKS
  GATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSS
  AVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTK
  LSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKR
  GAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEA
  RGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFP
  VKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTAL
  EMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQD
  KKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPT
  LAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKG
  NAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARA
  IQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILR
  IIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHL
  AESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKR
  KRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGS
  GLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAEC
  LVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEP
  VGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCAN
  VKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFY
  IGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKR
  GRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYW
  YYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVT
  AFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENE
  VGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAK
  VKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKR
  LEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWAL
  NEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFL
  FEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDY
  SSIEIMREKLLIAAREGQQSFHLS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284215
• ORF Size: 6075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001284215.2
• RefSeq Size: 9861 bp
• RefSeq ORF: 6078 bp
• Locus ID: 9320
• UniProt ID: Q14669
• Cytogenetics: 2q36.3
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 224.4 kDa
• Gene Summary: The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017]