MYO6 (NM_001300899) Human Tagged ORF Clone

CAT#: RG240080

MYO6 (tGFP-tagged) - Human myosin VI (MYO6), transcript variant 2

Product Images

Circular map for RG240080

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MYO6
• Synonyms: DFNA22; DFNB37
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240080 representing NM_001300899.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGGATGGAAAGCCCGTTTGGGCGCCACACCCTACAGATGGATTTCAGATGGGCAATATTGTGGAT
  ATTGGCCCCGACAGCTTAACAATTGAACCCTTGAATCAGAAAGGCAAGACATTTTTGGCTCTCATAAAC
  CAAGTGTTTCCTGCAGAAGAGGACAGTAAAAAAGATGTGGAAGATAACTGTTCACTAATGTATTTAAAT
  GAAGCCACACTGCTCCATAATATCAAAGTTCGATATAGTAAAGACAGAATTTATACATATGTCGCCAAC
  ATTCTGATTGCAGTGAATCCATACTTTGACATACCTAAAATATATTCTTCAGAAGCAATAAAGTCATAT
  CAAGGAAAATCTCTTGGGACAAGACCACCTCATGTCTTTGCAATTGCTGATAAAGCTTTTCGAGACATG
  AAGGTGCTCAAGATGAGTCAGTCTATCATTGTATCTGGAGAATCAGGAGCCGGCAAAACAGAAAATACA
  AAATTTGTTCTAAGATACCTGACTGAATCCTATGGAACAGGTCAAGATATTGATGACAGAATTGTTGAA
  GCTAACCCACTCCTAGAAGCCTTTGGAAATGCGAAGACTGTTCGCAACAATAATAGCAGTCGATTTGGG
  AAATTTGTAGAAATACATTTTAATGAAAAGAGCTCAGTTGTTGGAGGATTTGTTTCACATTATCTCCTA
  GAGAAATCTAGGATCTGTGTTCAAGGCAAAGAGGAAAGAAATTATCATATCTTTTATAGGTTGTGTGCT
  GGTGCTTCTGAAGATATTAGAGAAAAACTTCATTTGAGTTCACCAGATAATTTTCGGTATTTAAACCGA
  GGCTGCACTAGATACTTTGCTAACAAAGAAACTGACAAACAGATTTTACAGAACCGCAAAAGTCCTGAG
  TACCTTAAGGCAGGTTCTATGAAAGATCCTCTGCTAGATGACCATGGTGATTTTATTAGAATGTGCACG
  GCTATGAAAAAAATTGGTTTGGATGATGAAGAAAAGCTTGATCTCTTCCGGGTAGTAGCTGGCGTCCTG
  CACCTTGGAAATATTGATTTTGAGGAAGCTGGCAGCACTTCAGGTGGTTGTAATCTGAAGAATAAATCT
  GCTCAGTCTTTGGAATATTGTGCTGAATTACTGGGTTTGGACCAAGATGATCTTCGAGTAAGTTTGACC
  ACAAGAGTCATGCTAACAACAGCAGGGGGCACCAAAGGAACAGTTATAAAGGTACCTCTGAAAGTGGAG
  CAAGCAAACAATGCTCGTGATGCCCTGGCAAAGACAGTGTATAGCCATCTTTTTGATCATGTGGTAAAC
  AGAGTAAATCAGTGTTTTCCTTTTGAAACATCATCCTATTTTATTGGAGTCCTAGATATTGCTGGTTTT
  GAGTACTTTGAGCATAACAGTTTTGAACAATTTTGCATCAACTATTGCAATGAAAAACTTCAACAATTT
  TTTAATGAAAGGATTCTGAAGGAGGAACAAGAACTCTATCAAAAAGAAGGTTTAGGTGTTAATGAAGTG
  CATTATGTGGATAATCAGGACTGTATAGATTTAATTGAAGCCAAATTAGTGGGAATACTGGATATTTTG
  GATGAAGAAAATCGCCTTCCCCAGCCAAGTGATCAACACTTTACATCTGCAGTTCACCAAAAGCACAAG
  GATCATTTTCGACTCACTATTCCCAGAAAATCTAAGCTGGCAGTTCATAGGAATATCAGAGACGACGAA
  GGCTTCATTATCAGGCATTTTGCGGGGGCAGTGTGCTATGAAACAACCCAGTTTGTGGAGAAAAATAAT
  GATGCTTTACATATGTCTCTTGAATCCTTAATATGTGAATCCAGAGATAAGTTTATACGGGAATTATTT
  GAATCATCCACAAATAACAACAAAGATACTAAACAAAAAGCAGGAAAACTTAGCTTCATCAGCGTGGGA
  AACAAGTTTAAGACACAGTTAAATTTGCTTCTGGATAAACTTCGAAGTACTGGAGCAAGCTTTATTCGT
  TGCATCAAACCTAACTTAAAGATGACAAGCCACCACTTTGAAGGTGCTCAAATTCTGTCTCAGCTTCAG
  TGTTCAGGGATGGTGTCTGTTTTGGACTTGATGCAGGGTGGTTACCCATCACGAGCTTCATTTCATGAA
  CTCTACAACATGTACAAAAAGTATATGCCAGATAAACTTGCAAGATTGGATCCAAGACTATTTTGTAAG
  GCTTTGTTTAAAGCTTTGGGCTTAAATGAAAATGACTACAAGTTTGGGTTAACCAAAGTATTTTTTAGA
  CCTGGCAAGTTTGCAGAATTTGATCAGATCATGAAGTCTGACCCTGACCACTTAGCAGAGTTGGTTAAA
  AGAGTCAATCACTGGCTCACATGCAGTCGCTGGAAGAAAGTTCAGTGGTGCTCACTCTCAGTCATCAAA
  TTGAAAAACAAAATAAAATATCGAGCTGAAGCCTGCATTAAAATGCAAAAAACTATTCGAATGTGGCTT
  TGCAAGAGGAGACACAAACCTCGCATTGATGGTCTGGTTAAGGTGGGCACACTGAAAAAACGACTTGAT
  AAATTTAATGAGGTAGTCAGTGTGTTGAAAGATGGAAAACCCGAGATGAATAAACAGATCAAGAATCTG
  GAAATTTCTATTGATACTTTGATGGCCAAAATTAAGTCCACTATGATGACGCAGGAACAAATCCAGAAA
  GAATATGATGCACTGGTTAAAAGCTCAGAGGAACTCCTCAGTGCATTACAGAAAAAAAAACAGCAGGAA
  GAGGAAGCAGAAAGGCTGAGGCGTATTCAAGAAGAAATGGAAAAGGAAAGAAAAAGACGTGAAGAAGAC
  GAAAAACGTCGAAGAAAGGAAGAGGAGGAAAGGCGGATGAAACTTGAGATGGAAGCAAAGAGAAAACAA
  GAAGAAGAAGAGAGAAAGAAAAGGGAAGATGATGAAAAACGCATTCAAGCTGAAGTGGAGGCACAGCTG
  GCCCGACAGAAGGAGGAGGAATCCCAACAGCAAGCAGTTCTGGAGCAGGAGCGCAGGGACCGGGAGCTG
  GCCCTGAGGATTGCCCAGAGTGAAGCCGAGCTCATCAGTGATGAGGCCCAGGCCGACCTGGCGCTGCGG
  AGAGGTCCTGCTGTACTAGCCACCAAAGCAGCTGCTGGTACTAAGAAATATGATCTTAGTAAATGGAAA
  TATGCAGAACTACGTGATACCATCAATACTTCTTGTGATATTGAGCTCCTGGCAGCTTGCAGAGAAGAA
  TTTCATAGGAGACTAAAAGTGTATCATGCTTGGAAATCTAAGAACAAGAAGAGAAATACTGAAACAGAG
  CAACGTGCTCCAAAGTCTGTTACTGATTATGATTTTGCACCATTTTTGAACAATTCACCTCAGCAAAAC
  CCAGCAGCTCAGATTCCTGCCAGGCAGCGGGAGATTGAAATGAACCGACAGCAACGCTTCTTCCGCATC
  CCATTCATCCGCCCTGCCGACCAGTACAAAGACCCTCAGAGTAAGAAAAAAGGCTGGTGGTATGCCCAT
  TTTGATGGACCATGGATTGCCCGGCAAATGGAACTCCATCCTGACAAGCCACCCATCCTACTTGTGGCT
  GGTAAGGACGACATGGAGATGTGTGAGCTGAATCTTGAGGAGACTGGCCTGACTCGGAAGCGTGGTGCT
  GAGATCTTGCCAAGACAGTTTGAAGAAATCTGGGAACGCTGTGGAGGCATCCAGTACCTTCAGAATGCG
  ATTGAGAGCAGACAGGCTCGGCCCACCTATGCAACAGCCATGCTGCAGAGTCTGTTAAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240080
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEDGKPVWAPHPTDGFQMGNIVDIGPDSLTIEPLNQKGKTFLALINQVFPAEEDSKKDVEDNCSLMYLN
  EATLLHNIKVRYSKDRIYTYVANILIAVNPYFDIPKIYSSEAIKSYQGKSLGTRPPHVFAIADKAFRDM
  KVLKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDRIVEANPLLEAFGNAKTVRNNNSSRFG
  KFVEIHFNEKSSVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGASEDIREKLHLSSPDNFRYLNR
  GCTRYFANKETDKQILQNRKSPEYLKAGSMKDPLLDDHGDFIRMCTAMKKIGLDDEEKLDLFRVVAGVL
  HLGNIDFEEAGSTSGGCNLKNKSAQSLEYCAELLGLDQDDLRVSLTTRVMLTTAGGTKGTVIKVPLKVE
  QANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQF
  FNERILKEEQELYQKEGLGVNEVHYVDNQDCIDLIEAKLVGILDILDEENRLPQPSDQHFTSAVHQKHK
  DHFRLTIPRKSKLAVHRNIRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRELF
  ESSTNNNKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRSTGASFIRCIKPNLKMTSHHFEGAQILSQLQ
  CSGMVSVLDLMQGGYPSRASFHELYNMYKKYMPDKLARLDPRLFCKALFKALGLNENDYKFGLTKVFFR
  PGKFAEFDQIMKSDPDHLAELVKRVNHWLTCSRWKKVQWCSLSVIKLKNKIKYRAEACIKMQKTIRMWL
  CKRRHKPRIDGLVKVGTLKKRLDKFNEVVSVLKDGKPEMNKQIKNLEISIDTLMAKIKSTMMTQEQIQK
  EYDALVKSSEELLSALQKKKQQEEEAERLRRIQEEMEKERKRREEDEKRRRKEEEERRMKLEMEAKRKQ
  EEEERKKREDDEKRIQAEVEAQLARQKEEESQQQAVLEQERRDRELALRIAQSEAELISDEAQADLALR
  RGPAVLATKAAAGTKKYDLSKWKYAELRDTINTSCDIELLAACREEFHRRLKVYHAWKSKNKKRNTETE
  QRAPKSVTDYDFAPFLNNSPQQNPAAQIPARQREIEMNRQQRFFRIPFIRPADQYKDPQSKKKGWWYAH
  FDGPWIARQMELHPDKPPILLVAGKDDMEMCELNLEETGLTRKRGAEILPRQFEEIWERCGGIQYLQNA
  IESRQARPTYATAMLQSLLK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300899
• ORF Size: 3786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001300899.2
• RefSeq Size: 8609 bp
• RefSeq ORF: 3789 bp
• Locus ID: 4646
• UniProt ID: Q9UM54
• Cytogenetics: 6q14.1
• MW: 146.5 kDa
• Gene Summary: This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]