STAG3 (NM_001282716) Human Tagged ORF Clone

CAT#: RG240055

STAG3 (tGFP-tagged) - Human stromal antigen 3 (STAG3), transcript variant 2

Product Images

Circular map for RG240055

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: STAG3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240055 representing NM_001282716.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTTCCCCGTTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCT
  GCCAGTCTACCCTTTGATGACAGGGACTCAAACCATACCTCAGAGGGGAATGGCGACTCTTTGTTAGCT
  GATGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCACCG
  AAAACAACACCGGTGGCAAAACATCCAAAGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAG
  TCAGAGCCACCAGCCAATGATCTTTTCAATGCTGTGAAAGCCGCCAAAAGTGACATGCAGTCTTTGGTA
  GATGAGTGGCTGGATAGCTACAAGCAAGACCAGGATGCAGGATTTCTGGAGCTTGTTAACTTTTTCATC
  CAATCTTGCGGATGTAAAGGCATTGTGACCCCTGAGATGTTCAAGAAGATGTCCAACTCAGAGATCATC
  CAGCACCTAACAGAGCAGTTTAATGAGGACTCGGGGGACTACCCTCTCATAGCTCCAGGTCCATCCTGG
  AAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATTGGTCTGTCAGTGCCAGTACAGCCTCCTC
  TATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACTGGCCTCTCAGACTCACAAGTCCGCGCC
  TTCCGTCACACTAGCACCCTGGCTGCTATGAAACTGATGACCTCCCTGGTAAAAGTTGCCCTCCAACTG
  AGTGTGCACCAAGATAACAATCAGCGTCAGTATGAGGCTGAAAGAAACAAGGGGCCAGGGCAGAGGGCA
  CCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAGAGCTCCAAGAGCATCAAGAGGAGATTGAGGGG
  ATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGGATGTCCTTCCTGAGATCCGTGCT
  ATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCACGTCTTTCCTCACCGACAGCTATTTA
  AAATATATTGGTTGGACTCTGCATGATAAGCACCGAGAAGTCCGCCTGAAGTGTGTGAAGGCCCTGAAA
  GGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGAGCTCTTCACCAGCCGCTTCAAGGACCGGATG
  GTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTGGAGGCTGTCAGATTACTGATACTTATCCTT
  AAGAACATGGAAGGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCAGTTGTGTATGCCTCTCAT
  CGAGGCCTGGCCTCTGCCGCAGGCGAATTTCTGTACTGGAAACTCTTCTACCCTGAGTGCGAGATAAGA
  ATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAGGCGCCCAGAGGACTTTCTTCCAGCTTCTGCTGTCC
  TTCTTTGTGGAGAGCGAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGTGGGACTGTGCAGGG
  GCTCGGCTGAAGGACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAACCTGGGTGATGTG
  CAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTCAGAGGGGCACCCGCCTGTG
  GGCCGGGTCACTGGGAGGAAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATGACAGGGTGAAG
  TTGACTGAGCACCTCATCCCCCTGCTGCCCCAGCTCCTGGCCAAGTTCTCAGCTGATGCAGAGAAGGTC
  ACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACCTCCACATCTACTGCACTGGGCGCTTGGAGAAGCAC
  CTGGAGCTGTTCCTGCAGCAACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGCTTGAGGCT
  GGGGCGCATGCCCTCTACCTGCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTTTGCCCGC
  AGCCAGCTAGTAGATTTGCTGACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGTCGTCCTTC
  CTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAACGCCTCTCTGCCTTCTACAACACTCAT
  GACCTGACTCGCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGGAGAG
  GTTCCTCACCAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACCCAC
  ATTTCTAAATCAGATGCTTCCCAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGTGGCCTTCTGTGAA
  CTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGCTTTTGTCTTATTAAGTGAT
  CTACTTCTCATCTTTAGCCCTCAGATGATTGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTTT
  CCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGAC
  CTGGGCAGTGGTGATTCCCAGGAGGATCATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTA
  GCCGGGTTCTGCAAGCTGTTGCTTTATGGGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACAC
  TACAACAAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGAC
  CGAAGTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGCTGTACACAGAACTGCTGCAGGAGCAT
  GGGCCCCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGAGGTTTGCCTTG
  AGTTTTGGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGGAAGGCATCCAGTTC
  TCCTTGTCTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCCTCCAAATCTGGCATTCCTGGAGCTCCTT
  TCAGAGTTTTCCCCCCGACTCTTCCATCAGGACAAGCAGCTTTTACTGTCCTATCTAGAAAAGTGCCTG
  CAGCATGTCTCCCAGGCACCTGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCT
  GTGGAGAACACAGCAGAGACCAGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGGCCT
  GCCAAGCCTAACAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCGCCC
  ACGCCCACCCTCACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGGAA
  GAAGATGGCTCAGAGTTGGATTTTGCCCAGGGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTG
  GGTCCACAATATTTCCAGACTCCACACAACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGACTC
  AGCCTTATGGAAGAGGACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAATGAAGAACGGCAGGAT
  ACAGACATGCAAGCAAGTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAG
  CTGGATATTGAGGATTTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240055
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSSPLQRAVGDTKRALSASSSSSASLPFDDRDSNHTSEGNGDSLLADEDTDFEDSLNRNVKKRAAKRPP
  KTTPVAKHPKKGSRVVHRHSRKQSEPPANDLFNAVKAAKSDMQSLVDEWLDSYKQDQDAGFLELVNFFI
  QSCGCKGIVTPEMFKKMSNSEIIQHLTEQFNEDSGDYPLIAPGPSWKKFQGSFCEFVRTLVCQCQYSLL
  YDGFPMDDLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSVHQDNNQRQYEAERNKGPGQRA
  PERLESLLEKRKELQEHQEEIEGMMNALFRGVFVHRYRDVLPEIRAICIEEIGCWMQSYSTSFLTDSYL
  KYIGWTLHDKHREVRLKCVKALKGLYGNRDLTTRLELFTSRFKDRMVSMVMDREYDVAVEAVRLLILIL
  KNMEGVLTDADCESVYPVVYASHRGLASAAGEFLYWKLFYPECEIRMMGGREQRQSPGAQRTFFQLLLS
  FFVESELHDHAAYLVDSLWDCAGARLKDWEGLTSLLLEKDQNLGDVQESTLIEILVSSARQASEGHPPV
  GRVTGRKGLTSKERKTQADDRVKLTEHLIPLLPQLLAKFSADAEKVTPLLQLLSCFDLHIYCTGRLEKH
  LELFLQQLQEVVVKHAEPAVLEAGAHALYLLCNPEFTFFSRADFARSQLVDLLTDRFQQELEELLQSSF
  LDEDEVYNLAATLKRLSAFYNTHDLTRWELYEPCCQLLQKAVDTGEVPHQVILPALTLVYFSILWTLTH
  ISKSDASQKQLSSLRDRMVAFCELCQSCLSDVDTEIQEQAFVLLSDLLLIFSPQMIVGGRDFLRPLVFF
  PEATLQSELASFLMDHVFIQPGDLGSGDSQEDHLQIERLHQRRRLLAGFCKLLLYGVLEMDAASDVFKH
  YNKFYNDYGDIIKETLTRARQIDRSHCSRILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFAL
  SFGPQQLQNRDLVVMLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCL
  QHVSQAPGHPWGPVTTYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPP
  TPTLTSTAVKSRQPLWGLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRL
  SLMEEDEEEELEIQDESNEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282716
• ORF Size: 3675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282716.1, NP_001269645.1
• RefSeq Size: 4313 bp
• RefSeq ORF: 3678 bp
• Locus ID: 10734
• UniProt ID: Q9UJ98
• Cytogenetics: 7q22.1
• Protein Pathways: Oocyte meiosis
• MW: 139 kDa
• Gene Summary: The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014]