Metabotropic Glutamate Receptor 1 (GRM1) (NM_001278064) Human Tagged ORF Clone

CAT#: RG240038

GRM1 (tGFP-tagged) - Human glutamate receptor, metabotropic 1 (GRM1), transcript variant 3

Product Images

Circular map for RG240038

Specifications

Product Data

• Tag: TurboGFP
• Symbol: Metabotropic Glutamate Receptor 1
• Synonyms: GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44; SCAR13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240038 representing NM_001278064.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTCGGGCTCCTTTTGTTTTTTTTCCCAGCGATCTTTTTGGAGGTGTCCCTTCTCCCCAGAAGCCCC
  GGCAGGAAAGTGTTGCTGGCAGGAGCGTCGTCTCAGCGCTCGGTGGCCAGAATGGACGGAGATGTCATC
  ATTGGAGCCCTCTTCTCAGTCCATCACCAGCCTCCGGCCGAGAAAGTGCCCGAGAGGAAGTGTGGGGAG
  ATCAGGGAGCAGTATGGCATCCAGAGGGTGGAGGCCATGTTCCACACGTTGGATAAGATCAACGCGGAC
  CCGGTCCTCCTGCCCAACATCACCCTGGGCAGTGAGATCCGGGACTCCTGCTGGCACTCTTCCGTGGCT
  CTGGAACAGAGCATTGAGTTCATTAGGGACTCTCTGATTTCCATTCGAGATGAGAAGGATGGGATCAAC
  CGGTGTCTGCCTGACGGCCAGTCCCTCCCCCCAGGCAGGACTAAGAAGCCCATTGCGGGAGTGATCGGT
  CCCGGCTCCAGCTCTGTAGCCATTCAAGTGCAGAACCTGCTCCAGCTCTTCGACATCCCCCAGATCGCT
  TATTCAGCCACAAGCATCGACCTGAGTGACAAAACTTTGTACAAATACTTCCTGAGGGTTGTCCCTTCT
  GACACTTTGCAGGCAAGGGCCATGCTTGACATAGTCAAACGTTACAATTGGACCTATGTCTCTGCAGTC
  CACACGGAAGGGAATTATGGGGAGAGCGGAATGGACGCTTTCAAAGAGCTGGCTGCCCAGGAAGGCCTC
  TGTATCGCCCATTCTGACAAAATCTACAGCAACGCTGGGGAGAAGAGCTTTGACCGACTCTTGCGCAAA
  CTCCGAGAGAGGCTTCCCAAGGCTAGAGTGGTGGTCTGCTTCTGTGAAGGCATGACAGTGCGAGGACTC
  CTGAGCGCCATGCGGCGCCTTGGCGTCGTGGGCGAGTTCTCACTCATTGGAAGTGATGGATGGGCAGAC
  AGAGATGAAGTCATTGAAGGTTATGAGGTGGAAGCCAACGGGGGAATCACGATAAAGCTGCAGTCTCCA
  GAGGTCAGGTCATTTGATGATTATTTCCTGAAACTGAGGCTGGACACTAACACGAGGAATCCCTGGTTC
  CCTGAGTTCTGGCAACATCGGTTCCAGTGCCGCCTTCCAGGACACCTTCTGGAAAATCCCAACTTTAAA
  CGAATCTGCACAGGCAATGAAAGCTTAGAAGAAAACTATGTCCAGGACAGTAAGATGGGGTTTGTCATC
  AATGCCATCTATGCCATGGCACATGGGCTGCAGAACATGCACCATGCCCTCTGCCCTGGCCACGTGGGC
  CTCTGCGATGCCATGAAGCCCATCGACGGCAGCAAGCTGCTGGACTTCCTCATCAAGTCCTCATTCATT
  GGAGTATCTGGAGAGGAGGTGTGGTTTGATGAGAAAGGAGACGCTCCTGGAAGGTATGATATCATGAAT
  CTGCAGTACACTGAAGCTAATCGCTATGACTATGTGCACGTTGGAACCTGGCATGAAGGAGTGCTGAAC
  ATTGATGATTACAAAATCCAGATGAACAAGAGTGGAGTGGTGCGGTCTGTGTGCAGTGAGCCTTGCTTA
  AAGGGCCAGATTAAGGTTATACGGAAAGGAGAAGTGAGCTGCTGCTGGATTTGCACGGCCTGCAAAGAG
  AATGAATATGTGCAAGATGAGTTCACCTGCAAAGCTTGTGACTTGGGATGGTGGCCCAATGCAGATCTA
  ACAGGCTGTGAGCCCATTCCTGTGCGCTATCTTGAGTGGAGCAACATCGAATCCATTATAGCCATCGCC
  TTTTCATGCCTGGGAATCCTTGTTACCTTGTTTGTCACCCTAATCTTTGTACTGTACCGGGACACACCA
  GTGGTCAAATCCTCCAGTCGGGAGCTCTGCTACATCATCCTAGCTGGCATCTTCCTTGGTTATGTGTGC
  CCATTCACTCTCATTGCCAAACCTACTACCACCTCCTGCTACCTCCAGCGCCTCTTGGTTGGCCTCTCC
  TCTGCGATGTGCTACTCTGCTTTAGTGACTAAAACCAATCGTATTGCACGCATCCTGGCTGGCAGCAAG
  AAGAAGATCTGCACCCGGAAGCCCAGGTTCATGAGTGCCTGGGCTCAGGTGATCATTGCCTCAATTCTG
  ATTAGTGTGCAACTAACCCTGGTGGTAACCCTGATCATCATGGAACCCCCTATGCCCATTCTGTCCTAC
  CCAAGTATCAAGGAAGTCTACCTTATCTGCAATACCAGCAACCTGGGTGTGGTGGCCCCTTTGGGCTAC
  AATGGACTCCTCATCATGAGCTGTACCTACTATGCCTTCAAGACCCGCAACGTGCCCGCCAACTTCAAC
  GAGGCCAAATATATCGCGTTCACCATGTACACCACCTGTATCATCTGGCTAGCTTTTGTGCCCATTTAC
  TTTGGGAGCAACTACAAGATCATCACAACTTGCTTTGCAGTGAGTCTCAGTGTAACAGTGGCTCTGGGG
  TGCATGTTCACTCCCAAGATGTACATCATTATTGCCAAGCCTGAGAGGAATGTCCGCAGTGCCTTCACC
  ACCTCTGATGTTGTCCGCATGCATGTTGGCGATGGCAAGCTGCCCTGCCGCTCCAACACTTTCCTCAAC
  ATCTTCCGAAGAAAGAAGGCAGGGGCAGGGAATGCCAATTCTAATGGCAAGTCTGTGTCATGGTCTGAA
  CCAGGTGGAGGACAGGTGCCCAAGGGACAGCATATGTGGCACCGCCTCTCTGTGCACGTGAAGACCAAT
  GAGACGGCCTGCAACCAAACAGCCGTCATCAAGCCCCTCACTAAAAGTTACCAAGGCTCTGGCAAGAGC
  CTGACCTTTTCAGATACCAGCACCAAGACCCTTTACAACGTAGAGGAGGAGGAGGATGCCCAGCCGATT
  CGCTTTAGCCCGCCTGGTAGCCCTTCCATGGTGGTGCACAGGCGCGTGCCAAGCGCGGCGACCACTCCG
  CCTCTGCCGTCCCACCTGACCGCAGAGGAGACCCCCCTCTTCCTGGCCGAACCAGCCCTCCCCAAGGGC
  TTGCCCCCTCCTCTCCAGCAGCAGCAGCAACCCCCTCCACAGCAGAAATCGCTGATGGACCAGCTCCAG
  GGAGTGGTCAGCAACTTCAGTACCGCGATCCCGGATTTTCACGCGGTGCTGGCAGGCCCCGGTGGTCCC
  GGGAACGGGCTGCGGTCCCTGTACCCGCCCCCGCCACCTCCGCAGCACCTGCAGATGCTGCCGCTGCAG
  CTGAGCACCTTTGGGGAGGAGCTGGTCTCCCCGCCCGCGGACGACGACGACGACAGCGAGAGGTTTAAG
  CTCCTCCAGGAGTACGTGTATGAGCACGAGCGGGAAGGGAACACGGAAGAAGACGAACTGGAAGAGGAG
  GAGGAGGACCTGCAGGCGGCCAGCAAACTGACCCCGGATGATTCGCCTGCGCTGACGCCTCCGTCGCCT
  TTCCGCGACTCGGTGGCCTCGGGCAGCTCGGTGCCCAGCTCCCCCGTGTCCGAGTCGGTGCTCTGCACC
  CCTCCCAACGTATCCTACGCCTCTGTCATTCTGCGGGACTACAAGCAAAGCTCTTCCACCCTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240038
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVGLLLFFFPAIFLEVSLLPRSPGRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGE
  IREQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGIN
  RCLPDGQSLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPS
  DTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRK
  LRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSP
  EVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKRICTGNESLEENYVQDSKMGFVI
  NAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGSKLLDFLIKSSFIGVSGEEVWFDEKGDAPGRYDIMN
  LQYTEANRYDYVHVGTWHEGVLNIDDYKIQMNKSGVVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKE
  NEYVQDEFTCKACDLGWWPNADLTGCEPIPVRYLEWSNIESIIAIAFSCLGILVTLFVTLIFVLYRDTP
  VVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSK
  KKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPLGY
  NGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALG
  CMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKAGAGNANSNGKSVSWSE
  PGGGQVPKGQHMWHRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDTSTKTLYNVEEEEDAQPI
  RFSPPGSPSMVVHRRVPSAATTPPLPSHLTAEETPLFLAEPALPKGLPPPLQQQQQPPPQQKSLMDQLQ
  GVVSNFSTAIPDFHAVLAGPGGPGNGLRSLYPPPPPPQHLQMLPLQLSTFGEELVSPPADDDDDSERFK
  LLQEYVYEHEREGNTEEDELEEEEEDLQAASKLTPDDSPALTPPSPFRDSVASGSSVPSSPVSESVLCT
  PPNVSYASVILRDYKQSSSTL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278064
• ORF Size: 3582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001278064.2
• RefSeq Size: 6707 bp
• RefSeq ORF: 3585 bp
• Locus ID: 2911
• UniProt ID: Q13255
• Cytogenetics: 6q24.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• MW: 132.8 kDa
• Gene Summary: This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]