POLR1B (NM_001282774) Human Tagged ORF Clone

CAT#: RG239815

POLR1B (tGFP-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 4

Product Images

Circular map for RG239815

Specifications

Product Data

• Tag: TurboGFP
• Symbol: POLR1B
• Synonyms: A135; RPA2; RPA135; Rpo1-2; TCS4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239815 representing NM_001282774.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACTGACCCCTCT
  TATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTC
  AACTACGCTGTGCACGAGGGTCTCGGCCTCGCGGTGCAGGCTATACCTCCCTTTGAATTTGCTTTCAAA
  GATGAGCGTATCTCTTTTACTATTCTGGATGCTGTTATCAGTCCACCTACAGTTCCAAAAGGGACCATC
  TGCAAAGAGGCCAATGTTTATCCAGCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGACA
  GCTGATATCAACTGGGCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCC
  ATCATGGTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGCCCTCATTGAGCACCATGAG
  GAGGCAGAGGAAATGGGGGGCTATTTTATAATCAATGGCATTGAAAAAGTCATCCGAATGTTGATTATG
  CCTCGGAGAAATTTTCCCATTGCAATGATAAGACCAAAATGGAAAACCAGAGGGCCTGGTTATACTCAG
  TATGGAGTTTCAATGCACTGTGTGAGGGAAGAACATTCCGCTGTCAATATGAACCTCCACTACTTGGAA
  AATGGCACTGTTATGTTGAACTTTATTTACCGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTT
  AAGGCACTTGTCAGCTTTTCTGATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCT
  TTCCTTAGGAACTCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAG
  GTCCTTAACTACCTAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAATGAGCAA
  GCTGCGGAGTTCCTGTTTAACCAGTGCATCTGTATCCACTTGAAATCCAATACTGAAAAGTTTTATATG
  CTTTGTCTCATGACGCGAAAGCTCTTTGCTTTAGCCAAAGGAGAGTGCATGGAGGACAATCCTGATAGT
  TTGGTGAACCAGGAAGTCCTCACACCGGGTCAGCTCTTCCTTATGTTCCTGAAGGGGTCACTCCCATTG
  ATGGAGCTCCCCACCGATCATACAGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTT
  ATACCCATGACAGGAAAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTA
  CGGCCTGTGCAGAACTTAGCATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGATCTTCATGAAT
  GTCGCTATCTTTGAGGATGAAGTTTTTGCTGGAGTTACCACACACCAGGAACTCTTTCCACACAGCCTG
  CTGAGTGTGATTGCCAACTTCATCCCTTTCTCTGATCACAACCAGAGTCCACGGAACATGTACCAATGC
  CAGATGGGTAAGCAAACTATGGGCTTTCCACTTCTCACTTATCAAGACCGATCGGATAACAAACTGTAT
  CGTCTTCAGACTCCTCAGAGTCCCTTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTAT
  CCAATTGGGACCAATGCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATT
  GTGAATAAGGCCTCTTGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGACCTC
  TCTGAAAAAATTAAACAAGGAGATAGTAGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGCGTTCTG
  CAGAAGTTAGATGACGATGGATTGCCGTTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATTACAGC
  TACCTCAACCTCAACACCGGGGAAAGTTTTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGGAT
  AACATCAAAGTGTGCAGTAATGACACTGGGAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTG
  CCTCGGAACCCAACTATCGGAGATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTG
  TGGCCGGCTGAGGACATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGT
  TTTCCATCCCGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGT
  CTCTGCCATGATGCTACACCCTTCATCTTCTCAGAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATG
  TTAAAGGCTGCTGGCTACAATTTCTATGGCACCGAGAGGTTATATAGTGGCATCAGTGGGCTAGAACTG
  GAAGCAGACATCTTCATAGGAGTGGTTTATTATCAGCGCTTACGCCATATGGTCTCAGACAAATTTCAA
  GTAAGGACAACTGGAGCCCGAGACAGAGTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGA
  ATCCGTTTTGGGGAGATGGAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGC
  CTCTTCAACTGCTCAGATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTG
  TTGGAGAAGCCACCCCCTTCTTGGTCTGCCATGCGCAACAGAAAATACAACTGTACTCTGTGTAGTCGC
  AGTGACACTATCGATACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTAGCTGAACTGGCAGCTATG
  AACATCAAAGTGAAACTGGATGTTGTT
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239815
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDPGSRWRNLPSGPSLKHLTDPSYGIPREQQKAALQELTRAHVESFNYAVHEGLGLAVQAIPPFEFAFK
  DERISFTILDAVISPPTVPKGTICKEANVYPAECRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVP
  IMVKSKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQ
  YGVSMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDS
  FLRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYM
  LCLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKGSLPLMELPTDHTVLREKRIPPWMEVVL
  IPMTGKPSLYPGLFLFTTPCRLVRPVQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSL
  LSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNY
  PIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVL
  QKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRV
  PRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHG
  LCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQ
  VRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPL
  LEKPPPSWSAMRNRKYNCTLCSRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282774
• ORF Size: 2856 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282774.2
• RefSeq Size: 4878 bp
• RefSeq ORF: 2859 bp
• Locus ID: 84172
• UniProt ID: Q9H9Y6
• Cytogenetics: 2q14.1
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 108.3 kDa
• Gene Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]