RAD17 (NM_001278622) Human Tagged ORF Clone

CAT#: RG239274

RAD17 (tGFP-tagged) - Human RAD17 homolog (S. pombe) (RAD17), transcript variant 9

Product Images

Circular map for RG239274

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RAD17
• Synonyms: CCYC; HRAD17; R24L; RAD17SP; RAD24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239274 representing NM_001278622.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGCGTCTCTACT
  ATTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAA
  AGCAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCTTCCTTAGAACAGATTTATGGTTTAGAAAAT
  TCAAAAGAATATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCAGCATGAACTT
  GCTGTGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCA
  AAACAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATA
  CTATCAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAAAGAT
  GATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTT
  TTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGATCTGAGAACT
  GATAAGAAGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAA
  GTTCTAAGGAAGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGA
  GATAATAATCAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTC
  AACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAAT
  GGAGGAAAAATTACTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATC
  AGAAGTGCAATAAACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAAAAA
  GGAATGTCTTTAAAATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGGGTTTTT
  GAAAATCAAGAGGTCCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGGGGAAA
  ATTCTATATTGTAAAAGAGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATCAGAA
  TATGAACGGGATACATTACTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTA
  TTTAATTTATATCTTCACCAAAACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGT
  GAATTTCTGAGTTTTGCAGATATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGC
  ACATCTATAGCTACGAGAGGTGTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGA
  GGATCAAGTTTTCGACCCTTGCACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGC
  CTGGCAGCAAAAGCACTTTTTCCTGACTTCTGCCTACCAGCTTTATGCCGCCAAACTCAGCTATTGCCA
  TACCTTGCTCTACTAACCATTCCAATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGG
  CTCCCTCTGAAGCGACACTTTGGAAGATTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATA
  GACCCTGACAGCGGAGATGAAGCCCAGCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCC
  ACTCAAGCCACTGTGCCGGAAACCTGGTCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCT
  AGCCAGCCCCAGCCCTTTTCAGCCCAAGGAGACATGGAAGAAAACATAATAATAGAAGACTACGAGAGT
  GATGGGACA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239274
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MNQVTDWVDPSFDDFLECSGVSTITATSLGVNNSSHRRKNGPSTLESSRFPARKRGNLSSLEQIYGLEN
  SKEYLSENEPWVDKYKPETQHELAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKI
  LSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAVFKEFLLRATKYNKLQMLGDDLRT
  DKKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISF
  NPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKK
  GMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSE
  YERDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDWNTRSLLREYS
  TSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCRQTQLLP
  YLALLTIPMRNQAQISFIQDIGRLPLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEP
  TQATVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278622
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001278622.1, NP_001265551.1
• RefSeq Size: 3067 bp
• RefSeq ORF: 2013 bp
• Locus ID: 5884
• UniProt ID: O75943
• Cytogenetics: 5q13.2
• Protein Families: Druggable Genome
• MW: 75.9 kDa
• Gene Summary: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]