MEPE (NM_001291183) Human Tagged ORF Clone

CAT#: RG238885

MEPE (tGFP-tagged) - Human matrix extracellular phosphoglycoprotein (MEPE), transcript variant 6

Product Images

Circular map for RG238885

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MEPE
• Synonyms: OF45
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238885 representing NM_001291183.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGAGTTTTCTGTGTGGGACTACTCCTTTTCAGTGTGACCTGGGCAGCACCAACATTTCAACCACAG
  ACTGAGAAAACTAAGCAAAGCTGTGTGGAAGAGCAGAGGATAACATACAAGGGTCACTATGAGAAACAT
  GGGCATTATGTTTTTAAGTGTGTTTACATGTCACCTGAGAAGAAAAATCAAACTGATGTAAAGCAGGAA
  GAAAAAAACAAAGACAATATTGGTTTTCACCATTTGGGCAAGAGAATAAATCAAGAGCTATCATCTAAA
  GAAAATATTGTCCAGGAAAGAAAGAAAGATTTGTCCCTTTCTGAAGCCAGTGAGAATAAGGGAAGTAGT
  AAATCTCAAAATTATTTCACAAATAGACAGAGACTGAATAAAGAATATAGTATCAGTAACAAAGAGAAT
  ACTCACAATGGCCTGAGGATGTCAATTTATCCTAAGTCAACTGGGAATAAAGGGTTTGAGGATGGAGAT
  GATGCTATCAGCAAACTACATGACCAAGAAGAATATGGCGCAGCTCTCATCAGAAATAACATGCAACAT
  ATAATGGGGCCAGTGACTGCGATTAAACTCCTGGGGGAAGAAAACAAAGAGAACACACCTAGGAATGTT
  CTAAACATAATCCCAGCAAGTATGAATTATGCTAAAGCACACTCGAAGGATAAAAAGAAGCCTCAAAGA
  GATTCCCAAGCCCAGAAAAGTCCAGTAAAAAGCAAAAGCACCCATCGTATTCAACACAACATTGACTAC
  CTAAAACATCTCTCAAAAGTCAAAAAAATCCCCAGTGATTTTGAAGGCAGCGGTTATACAGATCTTCAA
  GAGAGAGGGGACAATGATATATCTCCTTTCAGTGGGGACGGCCAACCTTTTAAGGACATTCCTGGTAAA
  GGAGAAGCTACTGGTCCTGACCTAGAAGGCAAAGATATTCAAACAGGGTTTGCAGGCCCAAGTGAAGCT
  GAGAGTACTCATCTTGACACAAAAAAGCCAGGTTATAATGAGATCCCAGAGAGAGAAGAAAATGGTGGA
  AATACCATTGGAACTAGGGATGAAACTGCGAAAGAGGCAGATGCTGTTGATGTCAGCCTTGTAGAGGGC
  AGCAACGATATCATGGGTAGTACCAATTTTAAGGAGCTCCCTGGAAGAGAAGGAAACAGAGTGGATGCT
  GGCAGCCAAAATGCTCACCAAGGGAAGGTTGAGTTTCATTACCCTCCTGCACCCTCAAAAGAGAAAAGA
  AAAGAAGGCAGTAGTGATGCAGCTGAAAGTACCAACTATAATGAAATTCCTAAAAATGGCAAAGGCAGT
  ACCAGAAAGGGTGTAGATCATTCTAATAGGAACCAAGCAACCTTAAATGAAAAACAAAGGTTTCCTAGT
  AAGGGCAAAAGTCAGGGCCTGCCCATTCCTTCTCGTGGTCTTGATAATGAAATCAAAAACGAAATGGAT
  TCCTTTAATGGCCCCAGTCATGAGAATATAATAACACATGGCAGAAAATATCATTATGTACCCCACAGA
  CAAAATAATTCTACACGGAATAAGGGTATGCCACAAGGGAAAGGCTCCTGGGGTAGACAACCCCATTCC
  AACAGGAGGTTTAGTTCCCGTAGAAGGGATGACAGTAGTGAGTCATCTGACAGTGGCAGTTCAAGTGAG
  AGCGATGGTGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238885
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRVFCVGLLLFSVTWAAPTFQPQTEKTKQSCVEEQRITYKGHYEKHGHYVFKCVYMSPEKKNQTDVKQE
  EKNKDNIGFHHLGKRINQELSSKENIVQERKKDLSLSEASENKGSSKSQNYFTNRQRLNKEYSISNKEN
  THNGLRMSIYPKSTGNKGFEDGDDAISKLHDQEEYGAALIRNNMQHIMGPVTAIKLLGEENKENTPRNV
  LNIIPASMNYAKAHSKDKKKPQRDSQAQKSPVKSKSTHRIQHNIDYLKHLSKVKKIPSDFEGSGYTDLQ
  ERGDNDISPFSGDGQPFKDIPGKGEATGPDLEGKDIQTGFAGPSEAESTHLDTKKPGYNEIPEREENGG
  NTIGTRDETAKEADAVDVSLVEGSNDIMGSTNFKELPGREGNRVDAGSQNAHQGKVEFHYPPAPSKEKR
  KEGSSDAAESTNYNEIPKNGKGSTRKGVDHSNRNQATLNEKQRFPSKGKSQGLPIPSRGLDNEIKNEMD
  SFNGPSHENIITHGRKYHYVPHRQNNSTRNKGMPQGKGSWGRQPHSNRRFSSRRRDDSSESSDSGSSSE
  SDGD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291183
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001291183.1, NP_001278112.1
• RefSeq Size: 2054 bp
• RefSeq ORF: 1671 bp
• Locus ID: 56955
• UniProt ID: Q9NQ76
• Cytogenetics: 4q22.1
• Protein Families: Secreted Protein
• MW: 62.6 kDa
• Gene Summary: This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]