MYH (MUTYH) (NM_001293196) Human Tagged ORF Clone

CAT#: RG238178

MUTYH (tGFP-tagged) - Human mutY homolog (MUTYH), transcript variant gamma4

Product Images

Circular map for RG238178

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MYH
• Synonyms: MYH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238178 representing NM_001293196.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACCTGGACAGGCGGGCATATGCTGTGTGGGTCTCAGAGGTCATGCTGCAGCAGACCCAGGTTGCC
  ACTGTGATCAACTACTATACCGGATGGATGCAGAAGTGGCCTACACTGCAGGACCTGGCCAGTGCTTCC
  CTGGAGGAGGTGAATCAACTCTGGGCTGGCCTGGGCTACTATTCTCGTGGCCGGCGGCTGCAGGAGGGA
  GCTCGGAAGGTGGTAGAGGAGCTAGGGGGCCACATGCCACGTACAGCAGAGACCCTGCAGCAGCTCCTG
  CCTGGCGTGGGGCGCTACACAGCTGGGGCCATTGCCTCTATCGCCTTTGGCCAGGCAACCGGTGTGGTG
  GATGGCAACGTAGCACGGGTGCTGTGCCGTGTCCGAGCCATTGGTGCTGATCCCAGCAGCACCCTTGTT
  TCCCAGCAGCTCTGGGGTCTAGCCCAGCAGCTGGTGGACCCAGCCCGGCCAGGAGATTTCAACCAAGCA
  GCCATGGAGCTAGGGGCCACAGTGTGTACCCCACAGCGCCCACTGTGCAGCCAGTGCCCTGTGGAGAGC
  CTGTGCCGGGCACGCCAGAGAGTGGAGCAGGAACAGCTCTTAGCCTCAGGGAGCCTGTCGGGCAGTCCT
  GACGTGGAGGAGTGTGCTCCCAACACTGGACAGTGCCACCTGTGCCTGCCTCCCTCGGAGCCCTGGGAC
  CAGACCCTGGGAGTGGTCAACTTCCCCAGAAAGGCCAGCCGCAAGCCCCCCAGGGAGGAGAGCTCTGCC
  ACCTGTGTTCTGGAACAGCCTGGGGCCCTTGGGGCCCAAATTCTGCTGGTGCAGAGGCCCAACTCAGGT
  CTGCTGGCAGGACTGTGGGAGTTCCCGTCCGTGACCTGGGAGCCCTCAGAGCAGCTTCAGCGCAAGGCC
  CTGCTGCAGGAACTACAGCGTTGGGCTGGGCCCCTCCCAGCCACGCACCTCCGGCACCTTGGGGAGGTT
  GTCCACACCTTCTCTCACATCAAGCTGACATATCAAGTATATGGGCTGGCCTTGGAAGGGCAGACCCCA
  GTGACCACCGTACCACCAGGTGCTCGCTGGCTGACGCAGGAGGAATTTCACACCGCAGCTGTTTCCACC
  GCCATGAAAAAGGTTTTCCGTGTGTATCAGGGCCAACAGCCAGGGACCTGTATGGGTTCCAAAAGGTCC
  CAGGTGTCCTCTCCGTGCAGTCGGAAAAAGCCCCGCATGGGCCAGCAAGTCCTGGATAATTTCTTTCGG
  TCTCACATCTCCACTGATGCACACAGCCTCAACAGTGCAGCCCAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238178
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDLDRRAYAVWVSEVMLQQTQVATVINYYTGWMQKWPTLQDLASASLEEVNQLWAGLGYYSRGRRLQEG
  ARKVVEELGGHMPRTAETLQQLLPGVGRYTAGAIASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLV
  SQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSQCPVESLCRARQRVEQEQLLASGSLSGSP
  DVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSG
  LLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPATHLRHLGEVVHTFSHIKLTYQVYGLALEGQTP
  VTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFR
  SHISTDAHSLNSAAQ
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293196
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001293196.1, NP_001280125.1
• RefSeq Size: 1728 bp
• RefSeq ORF: 1290 bp
• Locus ID: 4595
• UniProt ID: Q9UIF7
• Cytogenetics: 1p34.1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair
• MW: 47.5 kDa
• Gene Summary: This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]