PMPCA (NM_001282946) Human Tagged ORF Clone

CAT#: RG238153

PMPCA (tGFP-tagged) - Human peptidase (mitochondrial processing) alpha (PMPCA), transcript variant 3

Product Images

Circular map for RG238153

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PMPCA
• Synonyms: Alpha-MPP; CLA1; CPD3; INPP5E; MAS2; P-55; SCAR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238153 representing NM_001282946.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGCGAAATACCTTAGTGGAATTGCTCACTTTTTGGAAAAATTGGCATTTTCGTCTACTGCTCGAT
  TTGACAGCAAAGATGAAATTCTGCTTACGTTGGAAAAGCATGGGGGTATCTGTGACTGCCAGACATCAA
  GACACCACCATGTATGCTGTGTCTGCTGATAGCAAAGGCTTGGACACGGTGGTTGCCTTACTGGCTGAT
  GTGGTTCTGCAGCCCCGGCTAACAGATGAAGAAGTCGAGATGACGCGGATGGCGGTCCAGTTTGAGCTG
  GAGGACCTGAACCTGCGGCCTGACCCAGAGCCACTTCTCACCGAGATGATTCATGAAGCGGCTTACAGG
  GAGAACACAGTTGGCCTCCACCGTTTCTGCCCCACAGAAAACGTAGCAAAGATCAACCGAGAGGTGCTG
  CATTCCTACCTGAGGAACTACTACACTCCCGACCGCATGGTGCTGGCCGGCGTGGGCGTGGAGCACGAG
  CATCTGGTGGACTGTGCCCGGAAGTACCTCCTGGGGGTCCAGCCGGCCTGGGGGAGCGCAGAGGCCGTG
  GATATTGACAGATCTGTGGCCCAGTACACTGGGGGGATTGCCAAGCTAGAAAGAGACATGTCCAATGTC
  AGCCTGGGCCCGACCCCCATCCCCGAGCTCACGCACATCATGGTTGGACTGGAGAGCTGCTCCTTCCTG
  GAGGAGGACTTCATCCCCTTTGCAGTGTTGAACATGATGATGGGCGGAGGTGGCTCCTTCTCGGCTGGT
  GGGCCCGGCAAGGGCATGTTCTCCAGGCTCTACCTCAACGTGCTCAACAGGCACCACTGGATGTATAAC
  GCGACCTCCTACCACCACAGCTACGAGGACACTGGCCTCCTTTGCATCCATGCCAGCGCCGACCCAAGA
  CAGGTTCGAGAAATGGTAGAAATCATCACAAAGGAGTTTATTTTAATGGGCGGAACCGTGGACACGGTG
  GAGCTGGAACGAGCCAAGACGCAGCTGACATCAATGCTCATGATGAACCTGGAATCCAGGCCTGTGATC
  TTCGAGGATGTGGGGAGGCAGGTGCTGGCCACTCGCTCCAGAAAGCTGCCGCACGAGCTGTGCACGCTC
  ATCCGCAACGTGAAGCCGGAAGATGTGAAGAGAGTCGCTTCTAAGATGCTCCGAGGGAAGCCGGCAGTG
  GCCGCCCTGGGTGACCTGACTGACCTGCCCACGTATGAGCACATCCAGACCGCCCTGTCGAGTAAGGAC
  GGGCGCCTGCCCAGGACGTACCGGCTCTTCCGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238153
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKRNTLVELLTFWKNWHFRLLLDLTAKMKFCLRWKSMGVSVTARHQDTTMYAVSADSKGLDTVVALLAD
  VVLQPRLTDEEVEMTRMAVQFELEDLNLRPDPEPLLTEMIHEAAYRENTVGLHRFCPTENVAKINREVL
  HSYLRNYYTPDRMVLAGVGVEHEHLVDCARKYLLGVQPAWGSAEAVDIDRSVAQYTGGIAKLERDMSNV
  SLGPTPIPELTHIMVGLESCSFLEEDFIPFAVLNMMMGGGGSFSAGGPGKGMFSRLYLNVLNRHHWMYN
  ATSYHHSYEDTGLLCIHASADPRQVREMVEIITKEFILMGGTVDTVELERAKTQLTSMLMMNLESRPVI
  FEDVGRQVLATRSRKLPHELCTLIRNVKPEDVKRVASKMLRGKPAVAALGDLTDLPTYEHIQTALSSKD
  GRLPRTYRLFR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282946
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282946.1, NP_001269875.1
• RefSeq Size: 2180 bp
• RefSeq ORF: 1278 bp
• Locus ID: 23203
• UniProt ID: Q10713
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome, Protease
• MW: 48.6 kDa
• Gene Summary: The protein encoded by this gene is found in the mitochondrion, where it represents the alpha subunit of a proteolytic heterodimer. This heterodimer is responsible for cleaving the transit peptide from nuclear-encoded mitochondrial proteins. Defects in this gene are a cause of spinocerebellar ataxia, autosomal recessive 2. [provided by RefSeq, Mar 2016]