TSEN34 (NM_001282333) Human Tagged ORF Clone

CAT#: RG237392

TSEN34 (tGFP-tagged) - Human TSEN34 tRNA splicing endonuclease subunit (TSEN34), transcript variant 4

Product Images

Circular map for RG237392

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TSEN34
• Synonyms: LENG5; PCH2C; SEN34; SEN34L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237392 representing NM_001282333.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGAGGATGCTGGTGGTGGAGGTGGCGAACGGCCGCTCCCTGGTGTGGGGAGCCGAGGCGGTGCAG
  GCCCTCCGGGAGCGCCTGGGTGTGGGGGGCCGCACGGTAGGCGCCCTGCCCCGCGGGCCCCGCCAGAAC
  TCGCGCCTGGGCCTCCCGCTGCTGCTGATGCCCGAAGAGGCGCGGCTCTTGGCCGAGATCGGCGCCGTG
  ACTCTGGTCAGCGCCCCGCGTCCAGACTCTCGGCACCACAGCCTGGCCCTGACATCCTTCAAGCGCCAG
  CAAGAGGAGAGCTTCCAGGAGCAGAGCGCCTTGGCAGCTGAGGCCCGGGAGACCCGTCGTCAGGAGCTC
  CTGGAGAAGATTACGGAGGGCCAGGCTGCTAAGAAGCAGAAACTAGAACAGGCTTCAGGGGCCAGCTCA
  AGCCAGGAGGCCGGCTCGAGCCAGGCTGCCAAAGAGGATGAGACCAGTGATGGCCAGGCTTCGGGAGAG
  CAGGAGGAAGCTGGCCCCTCGTCTTCCCAAGCAGGACCCTCAAATGGGGTAGCCCCCTTGCCCAGATCT
  GCTCTCCTTGTCCAGCTGGCCACTGCCAGGCCTCGACCGGTCAAGGCCAGGCCCCTGGACTGGCGTGTC
  CAGTCTAAAGACTGGCCCCACGCCGGCCGCCCTGCCCACGAGCTGCGCTACAGTATCTACAGAGACCTG
  TGGGAGCGAGGCTTCTTCCTCAGTGCGGCTGGCAAGTTCGGAGGTGACTTCCTGGTCTATCCTGGTGAC
  CCCCTCCGCTTCCACGCCCATTATATCGCTCAGTGCTGGGCCCCTGAGGACACCATCCCACTCCAAGAC
  CTGGTTGCTGCTGGGCGCCTTGGAACCAGCGTCAGAAAGACCCTGCTCCTCTGTTCTCCGCAGCCTGAT
  GGGTTTAAGTCTCAGGAGGTCTCAATAAACTTGGTATATAAATGTTCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237392
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRRMLVVEVANGRSLVWGAEAVQALRERLGVGGRTVGALPRGPRQNSRLGLPLLLMPEEARLLAEIGAV
  TLVSAPRPDSRHHSLALTSFKRQQEESFQEQSALAAEARETRRQELLEKITEGQAAKKQKLEQASGASS
  SQEAGSSQAAKEDETSDGQASGEQEEAGPSSSQAGPSNGVAPLPRSALLVQLATARPRPVKARPLDWRV
  QSKDWPHAGRPAHELRYSIYRDLWERGFFLSAAGKFGGDFLVYPGDPLRFHAHYIAQCWAPEDTIPLQD
  LVAAGRLGTSVRKTLLLCSPQPDGFKSQEVSINLVYKCS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282333
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282333.1, NP_001269262.1
• RefSeq Size: 1832 bp
• RefSeq ORF: 939 bp
• Locus ID: 79042
• Cytogenetics: 19q13.42
• MW: 33.9 kDa
• Gene Summary: This gene encodes a catalytic subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Oct 2009]