Nardilysin (NRDC) (NM_001242361) Human Tagged ORF Clone

CAT#: RG235163

NRD1 (tGFP-tagged) - Homo sapiens nardilysin (N-arginine dibasic convertase) (NRD1), transcript variant 3

Product Images

Circular map for RG235163

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nardilysin
• Synonyms: hNRD1; hNRD2; NRD1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235163 representing NM_001242361
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGTAAAACAGGAAATACAACAGATGATGAAGAAGAAGAGGAGGTGGAGGAAGAAGAAGAAGATG
  ATGATGAAGATTCTGGAGCTGAAATAGAAGATGACGATGAAGAGGGTTTTGATGATGAAGATGAGTTTGA
  TGATGAACATGATGATGATCTTGATACTGAGGATAATGAATTGGAAGAATTAGAAGAGAGAGCAGAAGCT
  AGAAAAAAAACTACTGAAAAACAGCAATTGCAGAGCCTGTTTTTGCTGTGGTCAAAGCTGACTGATAGAC
  TGTGGTTTAAGTCAACTTATTCAAAAATGTCTTCAACCCTGCTGGTCGAGACAAGAAATCTTTATGGGGT
  AGTTGGAGCTGAAAGCAGGTCTGCACCTGTTCAGCATTTGGCAGGATGGCAAGCGGAGGAGCAGCAGGGT
  GAAACTGACACAGTTCTGTCTGCAGCGGCTCTTTGTGTTGGAGTTGGGAGTTTCGCTGATCCAGATGACC
  TGCCGGGGCTGGCACACTTTTTGGAGCACATGGTATTCATGGGTAGTTTGAAATATCCAGATGAGAATGG
  ATTTGATGCCTTCCTGAAGAAGCATGGGGGTAGTGATAATGCCTCAACTGATTGTGAACGCACTGTCTTT
  CAGTTTGATGTCCAGAGGAAGTACTTCAAGGAAGCTCTTGATAGATGGGCGCAGTTCTTCATCCACCCAC
  TAATGATCAGAGATGCAATTGACCGTGAAGTTGAAGCTGTTGATAGTGAATATCAACTTGCAAGGCCTTC
  TGATGCAAACAGAAAGGAAATGTTGTTTGGAAGCCTTGCTAGACCTGGACATCCTATGGGAAAATTTTTT
  TGGGGAAATGCTGAGACGCTCAAGCATGAGCCAAGAAAGAATAATATTGATACACATGCTAGATTGAGAG
  AATTCTGGATGCGTTACTACTCTTCTCATTACATGACTTTAGTGGTTCAATCCAAAGAAACACTGGATAC
  TTTGGAAAAGTGGGTGACTGAAATCTTCTCTCAGATACCAAACAATGGGTTACCCAGACCAAACTTTGGC
  CATTTAACGGATCCATTTGACACACCAGCATTTAACAAACTTTATAGAGTTGTTCCAATCAGAAAAATTC
  ATGCTCTGACCATCACATGGGCACTTCCTCCTCAACAGCAACATTACAGGGTGAAGCCACTTCATTATAT
  ATCCTGGCTGGTTGGACATGAAGGCAAAGGCAGCATTCTTTCTTTCCTTAGGAAAAAATGCTGGGCTCTT
  GCACTGTTTGGTGGAAATGGTGAGACAGGATTTGAGCAAAATTCTACTTATTCAGTGTTCAGCATTTCTA
  TTACATTGACTGATGAGGGTTATGAACATTTTTATGAGGTTGCTTACACTGTCTTTCAGTATTTAAAAAT
  GCTGCAGAAGCTAGGCCCAGAAAAAAGAATTTTTGAAGAGATTCGGAAAATTGAGGATAATGAATTTCAT
  TACCAAGAACAGACAGATCCAGTTGAGTATGTGGAAAACATGTGTGAGAACATGCAGCTGTACCCATTGC
  AGGACATTCTCACTGGAGATCAGCTTCTTTTTGAATACAAGCCAGAAGTCATTGGTGAAGCCTTGAATCA
  GCTAGTTCCTCAAAAAGCAAATCTTGTTTTACTGTCTGGTGCTAATGAGGGAAAATGTGACCTCAAGGAG
  AAATGGTTTGGAACTCAATATAGTATAGAAGATATTGAAAACTCTTGGGCTGAACTGTGGAATAGTAATT
  TCGAATTAAATCCAGATCTTCATCTTCCAGCTGAAAACAAGTACATAGCCACGGACTTTACGTTGAAGGC
  TTTCGATTGCCCGGAAACAGAATACCCAGTTAAAATTGTGAATACTCCACAAGGTTGCCTGTGGTATAAG
  AAAGACAACAAATTCAAAATCCCCAAAGCATATATACGTTTCCATCTAATTTCACCGTTGATACAGAAAT
  CTGCAGCAAATGTGGTCCTCTTTGATATCTTTGTCAATATCCTTACGCATAACCTTGCGGAACCAGCTTA
  TGAAGCAGATGTGGCACAGCTGGAGTATAAACTGGTAGCTGGAGAACATGGTTTAATTATTCGAGTGAAA
  GGATTTAACCACAAACTACCTCTACTGTTTCAGCTCATTATTGACTACTTAGCTGAGTTCAATTCCACAC
  CAGCTGTCTTTACAATGATAACTGAGCAGTTGAAGAAGACCTACTTTAACATCCTCATCAAGCCCGAGAC
  TTTGGCCAAAGATGTACGGCTTTTAATCTTGGAATATGCCCGTTGGTCTATGATTGACAAGTACCAGGCT
  TTGATGGACGGCCTTTCCCTTGAGTCTCTGCTGAGCTTCGTCAAAGAATTCAAATCCCAGCTCTTTGTGG
  AGGGCCTGGTACAAGGGAATGTCACAAGCACAGAATCTATGGATTTCCTGAAATATGTTGTTGACAAACT
  AAACTTCAAGCCTCTGGAGCAGGAGATGCCTGTGCAGTTCCAGGTGGTAGAGCTGCCCAGTGGCCACCAT
  CTATGCAAAGTGAAAGCTCTGAACAAGGGTGATGCCAACTCTGAAGTCACTGTGTACTACCAGTCAGGTA
  CCAGGAGTCTAAGAGAATATACGCTTATGGAGCTGCTTGTGATGCACATGGAAGAACCTTGTTTTGACTT
  CCTTCGAACCAAGCAGACCCTTGGGTACCATGTCTACCCTACCTGTAGGAACACATCCGGGATTCTAGGA
  TTTTCTGTCACTGTGGGGACTCAGGCAACCAAATACAATTCTGAAGTTGTTGATAAGAAGATAGAAGAGT
  TTCTTTCTAGCTTTGAGGAGAAGATTGAGAACCTCACTGAAGAGGCATTCAACACCCAGGTCACAGCTCT
  CATCAAGCTGAAGGAGTGTGAGGATACCCACCTTGGGGAGGAGGTGGATAGGAACTGGAATGAAGTGGTT
  ACACAGCAGTACCTCTTTGACCGCCTTGCCCACGAGATTGAAGCACTGAAGTCATTCTCAAAATCAGACC
  TGGTCAACTGGTTCAAGGCCCATAGAGGGCCAGGAAGTAAAATGCTCAGCGTTCATGTTGTTGGATATGG
  GAAGTATGAACTGGAAGAGGATGGTACCCCTTCTAGTGAGGATTCAAATTCTTCTTGTGAAGTGATGCAG
  CTGACCTACCTGCCAACCTCTCCTCTGCTGGCAGATTGTATCATCCCCATTACTGATATCAGGGCTTTCA
  CAACAACACTCAACCTTCTCCCCTACCATAAAATAGTCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235163 representing NM_001242361
  Red=Cloning site Green=Tags(s)
  
  MEGKTGNTTDDEEEEEVEEEEEDDDEDSGAEIEDDDEEGFDDEDEFDDEHDDDLDTEDNELEELEERAEA
  RKKTTEKQQLQSLFLLWSKLTDRLWFKSTYSKMSSTLLVETRNLYGVVGAESRSAPVQHLAGWQAEEQQG
  ETDTVLSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVF
  QFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFF
  WGNAETLKHEPRKNNIDTHARLREFWMRYYSSHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPRPNFG
  HLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSFLRKKCWAL
  ALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAYTVFQYLKMLQKLGPEKRIFEEIRKIEDNEFH
  YQEQTDPVEYVENMCENMQLYPLQDILTGDQLLFEYKPEVIGEALNQLVPQKANLVLLSGANEGKCDLKE
  KWFGTQYSIEDIENSWAELWNSNFELNPDLHLPAENKYIATDFTLKAFDCPETEYPVKIVNTPQGCLWYK
  KDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVK
  GFNHKLPLLFQLIIDYLAEFNSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYARWSMIDKYQA
  LMDGLSLESLLSFVKEFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFKPLEQEMPVQFQVVELPSGHH
  LCKVKALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILG
  FSVTVGTQATKYNSEVVDKKIEEFLSSFEEKIENLTEEAFNTQVTALIKLKECEDTHLGEEVDRNWNEVV
  TQQYLFDRLAHEIEALKSFSKSDLVNWFKAHRGPGSKMLSVHVVGYGKYELEEDGTPSSEDSNSSCEVMQ
  LTYLPTSPLLADCIIPITDIRAFTTTLNLLPYHKIVK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242361
• ORF Size: 3261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242361.1, NP_001229290.1
• RefSeq Size: 3435 bp
• RefSeq ORF: 3264 bp
• Locus ID: 4898
• UniProt ID: O43847
• Cytogenetics: 1p32.3
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a zinc-dependent endopeptidase that cleaves peptide substrates at the N-terminus of arginine residues in dibasic moieties and is a member of the peptidase M16 family. This protein interacts with heparin-binding EGF-like growth factor and plays a role in cell migration and proliferation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]