ARPP21 (NM_001267619) Human Tagged ORF Clone

CAT#: RG234922

ARPP21 (tGFP-tagged) - Homo sapiens cAMP-regulated phosphoprotein, 21kDa (ARPP21), transcript variant 8

Product Images

Circular map for RG234922

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARPP21
• Synonyms: ARPP-21; R3HDM3; RCS; TARPP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234922 representing NM_001267619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCAAGGAGACCTGAATCAGGCAATAGCAGAGGAAGGAGGGACTGAGCAGGAGACGGCCACTC
  CAGAGAACGGCATTGTTAAATCAGAAAGTCTGGATGAAGAGGAGAAACTGGAACTGCAGAGGCGGCTGGA
  GGCTCAGAATCAAGAAAGAAGAAAATCCAAGTCAGGAGCAGGAAAAGGTAAACTGACTCGCAGCCTTGCT
  GTCTGTGAGGAATCTTCTGCCAGACCAGGAGGTGAAAGTCTTCAGGATCAGGAATCAATTCATTTACAGC
  TTTCCAGTTTTTCCAGCCTGCAAGAGGAGGATAAATCTAGGAAAGATGACTCTGAAAGAGAAAAAGAAAA
  GGATAAAAACAAAGATAAAACCTCTGAAAAACCCAAGATCAGAATGTTATCAAAAGATTGCAGCCAAGAA
  TACACGGATTCTACAGGCATAGACTTACACGAGTTTCTGATTAACACATTAAAGAATAATTCCAGGGACA
  GGATGATACTTTTGAAAATGGAGCAGGAAATTATTGATTTCATTGCTGACAACAATAATCATTATAAAAA
  GTTCCCTCAGATGTCATCGTATCAGAGGATGCTTGTCCATCGAGTGGCAGCTTATTTTGGATTGGATCAC
  AATGTGGATCAAACAGGAAAATCTGTTATCATCAACAAGACCAGCAGCACCAGAATACCAGAGCAAAGGT
  TTTGTGAACATTTAAAAGATGAAAAAGGTGAAGAATCCCAGAAGCGGTTTATCTTGAAGCGAGATAACTC
  TAGTATTGATAAAGAAGACAATCAGTCAGTTTGCTCCCAGGAAAGCCTTTTTGTGGAAAACAGTAGGCTC
  TTGGAAGACAGTAACATATGCAATGAGACCTATAAGAAAAGACAGCTCTTTCGGGGCAACAGAGATGGCT
  CAGGGAGAACATCTGGGAGTCGACAGAGCAGCTCAGAAAATGAACTCAAGTGGTCTGACCACCAAAGGGC
  CTGGAGCAGCACAGACTCCGACAGTTCCAACCGCAATCTAAAGCCCGCCATGACCAAGACGGCGAGTTTT
  GGGGGCATCACGGTGCTGACCAGGGGTGACAGCACTTCCAGTACTAGGAGTACCGGGAAGCTGTCCAAAG
  CAGGTTCCGAGTCTTCCAGCAGTGCAGGCTCCTCAGGATCGCTGTCCCGCACCCATCCACCTCTCCAGAG
  CACACCCCTAGTCTCAGGTGTGGCAGCTGGCTCTCCAGGCTGTGTGCCTTATCCAGAGAATGGAATAGGG
  GGCCAGGTTGCTCCCAGCAGCACCAGCTACATCCTCCTTCCACTTGAAGCTGCAACAGGCATCCCGCCTG
  GAAGCATCCTTCTTAATCCACACACAGGCCAGCCCTTTGTGAATCCCGATGGAACTCCTGCAATATACAA
  CCCACCCACCAGTCAGCAGCCCCTGCGAAGCGCCATGGTGGGGCAGTCCCAACAGCAGCCACCACAGCAG
  CAGCCCTCCCCGCAGCCCCAACAGCAGGTCCAGCCACCGCAGCCACAGATGGCAGGCCCTCTGGTCACTC
  AGTCTGTCCAGGGGCTGCAGGCTTCCTCCCAGTCAGTGCAATATCCAGCAGTCTCTTTTCCTCCCCAGCA
  CCTCCTACCTGTGTCTCCAACGCAGCACTTTCCCATGAGAGATGATGTGGCAACACAGTTTGGCCAGATG
  ACCCTGAGCCGGCAGTCCTCGGGGGAGACTCCTGAACCCCCATCAGGTCCTGTCTACCCATCCTCCCTTA
  TGCCACAGCCGGCCCAGCAGCCCAGCTATGTAATCGCCTCTACAGGCCAGCAGCTTCCTACAGGAGGATT
  CTCAGGCTCTGGCCCTCCCATCTCCCAGCAGGTCCTCCAGCCCCCTCCCTCACCACAGGGATTTGTGCAA
  CAGCCTCCGCCTGCACAGATGCCTGTATATTATTACCCATCTGGTCAGTACCCTACCTCAACCACGCAAC
  AGTACCGGCCCATGGCCCCGGTTCAGTACAACGCTCAGAGGAGTCAACAGATGCCACAGGCAGCACAGCA
  AGCAGGTTACCAGCCAGTCTTGTCTGGTCAACAGGGATTCCAAGGCCTAATAGGAGTGCAGCAGCCACCT
  CAGAGTCAGAACGTGATAAATAACCAACAAGGAACTCCGGTGCAAAGCGTGATGGTTTCCTACCCAACAA
  TGTCTTCTTATCAGGTGCCAATGACCCAGGGTTCTCAAGGACTGCCCCAGCAGTCATACCAACAGCCAAT
  CATGCTACCTAACCAGGCAGGTCAAGGGTCACTCCCAGCCACTGGAATGCCTGTTTACTGTAATGTCACA
  CCGCCCACCCCTCAGAACAACCTTAGGCTGATTGGCCCACACTGCCCCTCCAGCACTGTCCCAGTGATGT
  CAGCTAGCTGCAGAACAAACTGTGCAAGTATGAGCAATGCTGGTTGGCAGGTCAAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234922 representing NM_001267619
  Red=Cloning site Green=Tags(s)
  
  MSEQGDLNQAIAEEGGTEQETATPENGIVKSESLDEEEKLELQRRLEAQNQERRKSKSGAGKGKLTRSLA
  VCEESSARPGGESLQDQESIHLQLSSFSSLQEEDKSRKDDSEREKEKDKNKDKTSEKPKIRMLSKDCSQE
  YTDSTGIDLHEFLINTLKNNSRDRMILLKMEQEIIDFIADNNNHYKKFPQMSSYQRMLVHRVAAYFGLDH
  NVDQTGKSVIINKTSSTRIPEQRFCEHLKDEKGEESQKRFILKRDNSSIDKEDNQSVCSQESLFVENSRL
  LEDSNICNETYKKRQLFRGNRDGSGRTSGSRQSSSENELKWSDHQRAWSSTDSDSSNRNLKPAMTKTASF
  GGITVLTRGDSTSSTRSTGKLSKAGSESSSSAGSSGSLSRTHPPLQSTPLVSGVAAGSPGCVPYPENGIG
  GQVAPSSTSYILLPLEAATGIPPGSILLNPHTGQPFVNPDGTPAIYNPPTSQQPLRSAMVGQSQQQPPQQ
  QPSPQPQQQVQPPQPQMAGPLVTQSVQGLQASSQSVQYPAVSFPPQHLLPVSPTQHFPMRDDVATQFGQM
  TLSRQSSGETPEPPSGPVYPSSLMPQPAQQPSYVIASTGQQLPTGGFSGSGPPISQQVLQPPPSPQGFVQ
  QPPPAQMPVYYYPSGQYPTSTTQQYRPMAPVQYNAQRSQQMPQAAQQAGYQPVLSGQQGFQGLIGVQQPP
  QSQNVINNQQGTPVQSVMVSYPTMSSYQVPMTQGSQGLPQQSYQQPIMLPNQAGQGSLPATGMPVYCNVT
  PPTPQNNLRLIGPHCPSSTVPVMSASCRTNCASMSNAGWQVKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267619
• ORF Size: 2439 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267619.1, NP_001254548.1
• RefSeq Size: 3209 bp
• RefSeq ORF: 2442 bp
• Locus ID: 10777
• UniProt ID: Q9UBL0
• Cytogenetics: 3p22.3
• Gene Summary: This gene encodes a cAMP-regulated phosphoprotein. The encoded protein is enriched in the caudate nucleus and cerebellar cortex. A similar protein in mouse may be involved in regulating the effects of dopamine in the basal ganglia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]