Miz1 (ZBTB17) (NM_001242884) Human Tagged ORF Clone

CAT#: RG234774

ZBTB17 (tGFP-tagged) - Homo sapiens zinc finger and BTB domain containing 17 (ZBTB17), transcript variant 1

Product Images

Circular map for RG234774

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Miz1
• Synonyms: MIZ-1; pHZ-67; ZNF60; ZNF151
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234774 representing NM_001242884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGCTGGCCGTGGCCACTTTCCTCCAAATGCAGGACATCATCACGGCCTGCCATGCCCTCAAGTC
  ACTTGCTGAGCCGGCTACCAGCCCTGGGGGAAATGCGGAGGCCTTGGCCACAGAAGGTCTGCCCTGTTCC
  ATCTCCAGGAGGGGACAAGAGAGCCAAAGAGGAGAAGGTGGCCACCAGCACGCTGAGCAGGCTGGAGCAG
  GCAGGACGCAGCACACCCATAGGCCCCAGCAGGGACCTCAAGGAGGAGCGCGGCGGTCAGGCCCAGAGTG
  CGGCCAGCGGTGCAGAGCAGACAGAGAAAGCCGATGCGCCCCGGGAGCCGCCGCCTGTGGAGCTCAAGCC
  AGACCCCACGAGTGGCATGGCTGCTGCAGAAGCTGAGGCCGCTTTGTCCGAGAGCTCGGAGCAAGAAATG
  GAGGTGGAGCCCGCCCGGAAAGGGGAAGAGGAGCAAAAGGAGCAAGAGGAGCAAGAGGAGGAGGGCGCAG
  GGCCAGCTGAGGTCAAGGAGGAGGGTTCCCAGCTGGAGAACGGAGAGGCCCCCGAGGAGAACGAGAATGA
  GGAGTCAGCGGGCACAGACTCGGGGCAGGAGCTCGGCTCCGAGGCCCGGGGCCTGCGCTCAGGCACCTAC
  GGCGACCGCACGGAGTCCAAGGCCTACGGCTCCGTCATCCACAAGTGCGAGGACTGTGGGAAGGAGTTCA
  CGCACACGGGGAACTTCAAGCGGCACATCCGCATCCACACGGGGGAGAAGCCCTTCTCGTGCCGGGAGTG
  CAGCAAGGCCTTTTCCGACCCGGCCGCGTGCAAGGCCCATGAGAAGACGCACAGCCCTCTGAAGCCCTAC
  GGCTGCGAGGAGTGCGGGAAGAGCTACCGCCTCATCAGCCTGCTGAACCTGCACAAGAAGCGGCACTCGG
  GCGAGGCGCGCTACCGCTGCGAGGACTGCGGCAAGCTCTTCACCACCTCGGGCAACCTCAAGCGCCACCA
  GCTGGTGCACAGCGGCGAGAAGCCCTACCAGTGCGACTACTGCGGCCGCTCCTTCTCCGACCCCACTTCC
  AAGATGCGCCACCTGGAGACCCACGACACGGACAAGGAGCACAAGTGCCCACACTGCGACAAGAAGTTCA
  ACCAGGTAGGGAACCTGAAGGCCCACCTGAAGATCCACATCGCTGACGGGCCCCTCAAGTGCCGAGAGTG
  TGGGAAGCAGTTCACCACCTCAGGGAACCTGAAGCGGCACCTTCGGATCCACAGCGGGGAGAAGCCCTAC
  GTGTGCATCCACTGCCAGCGACAGTTTGCAGACCCCGGCGCTCTGCAGCGGCACGTCCGCATTCACACAG
  GTGAGAAGCCATGCCAGTGTGTGATGTGCGGTAAGGCCTTCACCCAGGCCAGCTCCCTCATCGCCCACGT
  GCGCCAGCACACCGGGGAGAAGCCCTACGTCTGCGAGCGCTGCGGCAAGAGATTCGTCCAGTCCAGCCAG
  TTGGCCAATCATATTCGCCACCACGACAACATCCGCCCACACAAGTGCAGCGTGTGCAGCAAGGCCTTCG
  TGAACGTGGGGGACCTGTCCAAGCACATCATCATTCACACTGGAGAGAAGCCTTACCTGTGTGATAAGTG
  TGGGCGTGGCTTCAACCGGGTAGACAACCTGCGCTCCCACGTGAAGACCGTGCACCAGGGCAAGGCAGGC
  ATCAAGATCCTGGAGCCCGAGGAGGGCAGTGAGGTCAGCGTGGTCACTGTGGATGACATGGTCACGCTGG
  CTACCGAGGCACTGGCAGCGACAGCCGTCACTCAGCTCACAGTGGTGCCGGTGGGAGCTGCAGTGACAGC
  CGATGAGACGGAAGTCCTGAAGGCCGAGATCAGCAAAGCTGTGAAGCAAGTGCAGGAAGAAGACCCCAAC
  ACTCACATCCTCTACGCCTGTGACTCCTGTGGGGACAAGTTTCTGGATGCCAACAGCCTGGCTCAGCATG
  TGCGAATCCACACAGCCCAGGCACTGGTCATGTTCCAGACAGACGCGGACTTCTATCAGCAGTATGGGCC
  AGGTGGCACGTGGCCTGCCGGGCAGGTGCTGCAGGCTGGGGAGCTGGTCTTCCGCCCTCGCGACGGGGCT
  GAGGGCCAGCCCGCACTGGCAGAGACCTCCCCTACAGCTCCTGAATGTCCCCCGCCTGCCGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234774 representing NM_001242884
  Red=Cloning site Green=Tags(s)
  
  MMCWPWPLSSKCRTSSRPAMPSSHLLSRLPALGEMRRPWPQKVCPVPSPGGDKRAKEEKVATSTLSRLEQ
  AGRSTPIGPSRDLKEERGGQAQSAASGAEQTEKADAPREPPPVELKPDPTSGMAAAEAEAALSESSEQEM
  EVEPARKGEEEQKEQEEQEEEGAGPAEVKEEGSQLENGEAPEENENEESAGTDSGQELGSEARGLRSGTY
  GDRTESKAYGSVIHKCEDCGKEFTHTGNFKRHIRIHTGEKPFSCRECSKAFSDPAACKAHEKTHSPLKPY
  GCEECGKSYRLISLLNLHKKRHSGEARYRCEDCGKLFTTSGNLKRHQLVHSGEKPYQCDYCGRSFSDPTS
  KMRHLETHDTDKEHKCPHCDKKFNQVGNLKAHLKIHIADGPLKCRECGKQFTTSGNLKRHLRIHSGEKPY
  VCIHCQRQFADPGALQRHVRIHTGEKPCQCVMCGKAFTQASSLIAHVRQHTGEKPYVCERCGKRFVQSSQ
  LANHIRHHDNIRPHKCSVCSKAFVNVGDLSKHIIIHTGEKPYLCDKCGRGFNRVDNLRSHVKTVHQGKAG
  IKILEPEEGSEVSVVTVDDMVTLATEALAATAVTQLTVVPVGAAVTADETEVLKAEISKAVKQVQEEDPN
  THILYACDSCGDKFLDANSLAQHVRIHTAQALVMFQTDADFYQQYGPGGTWPAGQVLQAGELVFRPRDGA
  EGQPALAETSPTAPECPPPAE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242884
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242884.1, NP_001229813.1
• RefSeq Size: 2582 bp
• RefSeq ORF: 2166 bp
• Locus ID: 7709
• UniProt ID: Q13105
• Cytogenetics: 1p36.13
• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle
• Gene Summary: This gene encodes a zinc finger protein involved in the regulation of c-myc. The symbol MIZ1 has also been associated with PIAS2 which is a different gene located on chromosome 18. [provided by RefSeq, Jul 2008]